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  • 51.
    Lind-Halldén, Christina
    et al.
    Kristianstad University, Faculty of Natural Science, Forskningsmiljön Biomedicin. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Manderstedt, Eric
    Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Carlberg, Daniel
    Kristianstad University, Faculty of Natural Science, Forskningsmiljön Biomedicin. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Lethagen, Stefan
    Skåne University Hospital in Malmö.
    Halldén, Christer
    Kristianstad University, Faculty of Natural Science, Forskningsmiljön Biomedicin. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Genetic variation in the syntaxin-binding protein STXBP5 in type 1 von Willebrand disease patients2018In: Thrombosis and haemostasis, ISSN 2567-689X, Vol. 118, no 8, p. 1382-1389Article in journal (Refereed)
    Abstract [en]

    von Willebrand factor (VWF) levels in healthy individuals and in patients with type 1 von Willebrand disease (VWD) are influenced by genetic variation in several genes, for example, VWF, ABO and STXBP5. Here, we comprehensively screen for STXBP5 variants and investigate their association with type 1 VWD in Swedish patients and controls. The coding region of the STXBP5 gene was re-sequenced in 107 type 1 VWD patients and the detected variants were genotyped in the type 1 VWD population and a Swedish control population (464 individuals). The functional effects of missense alleles were predicted in silico and the pattern of genetic variation in STXBP5 was analysed. Re-sequencing of 107 type 1 VWD patients identified three missense and three synonymous variants in the coding sequence of STXBP5. The low-frequency missense variants rs144099092 (0.005) and rs148830578 (0.029) were predicted to be damaging, but were not accumulated in patients. No other rare candidate mutations were detected. STXBP5 showed a high level of linkage disequilibrium and a low overall nucleotide diversity of π = 3.2 × 10-4 indicating intolerance to variants affecting protein function. Three previously type 1 VWD-associated single nucleotide polymorphisms were located on one haplotype that showed an increased frequency in patients versus controls. No differences in messenger ribonucleic acid abundance among haplotypes could be found using Genotype-Tissue Expression project data. In conclusion, a haplotype containing the STXBP5 Asn436Ser (rs1039084) mutation is associated with type 1 VWD and no rare STXBP5 mutations contribute to type 1 VWD in the Swedish population.

  • 52.
    Manderstedt, Eric
    et al.
    Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Lind-Halldén, Christina
    Kristianstad University, Forskningsmiljön Biomedicin. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Lethagen, Stefan
    Danmark & Lund University.
    Halldén, Christer
    Kristianstad University, Forskningsmiljön Biomedicin. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Genetic variation in the C-type lectin receptor CLEC4M in type 1 von Willebrand Disease patients2018In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 13, no 2Article in journal (Refereed)
    Abstract [en]

    von Willebrand factor (VWF) levels in healthy individuals and in patients with type 1 von Willebrand disease (VWD) are influenced by genetic variation in several genes, e.g. VWF, ABO, STXBP5 and CLEC4M. This study aims to screen comprehensively for CLEC4M variants and investigate their association with type 1 VWD in the Swedish population. In order to screen for CLEC4M variants, the CLEC4M gene region was re-sequenced and the polymorphic neck region was genotyped in 106 type 1 VWD patients from unrelated type 1 VWD families. Single nucleotide variants (SNV) and variable number tandem repeat (VNTR) allele and genotype frequencies were then compared with 294 individuals from the 1000Genomes project and 436 Swedish control individuals. Re-sequencing identified a total of 42 SNVs. Rare variants showed no accumulation in type 1 VWD patients and are not thought to contribute substantially to type 1 VWD. The only missense mutation (rs2277998, NP_001138379.1:p.Asp224Asn) had a higher frequency in type 1 VWD patients than in controls (4.9%). The VNTR genotypes 57 and 67 were observed at higher frequencies than expected in type 1 VWD patients (6.4% and 6.2%) and showed an increase in patients compared with controls (7.4% and 3.1%). Strong linkage disequilibrium in the CLEC4M region makes it difficult to distinguish between the effect of the missense mutation and the VNTR genotypes. In conclusion, heterozygous VNTR genotypes 57 and 67 of CLEC4M were highly enriched and are the most likely mechanism through which CLEC4M contributes to disease in the Swedish type 1 VWD population.

  • 53.
    Manderstedt, Eric
    et al.
    Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Lind-Halldén, Christina
    Kristianstad University, Forskningsmiljön Biomedicin. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Lethagen, Stefan
    Danmark.
    Halldén, Christer
    Kristianstad University, Forskningsmiljön Biomedicin. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Genetic variation in the von Willebrand factor gene in Swedish von Willebrand disease patients2018In: TH Open, ISSN 2512-9465, Vol. 2, no 1, p. 39-48Article in journal (Refereed)
    Abstract [en]

    von Willebrand factor (VWF) level and function are influenced by genetic variation in VWF and several other genes in von Willebrand disease type 1 (VWD1) patients. This study comprehensively screened for VWF variants and investigated the presence of ABO genotypes and common and rare VWF variants in Swedish VWD1 patients. The VWF gene was resequenced using Ion Torrent and Sanger sequencing in 126 index cases historically diagnosed with VWD. Exon 7 of the ABO gene was resequenced using Sanger sequencing. Multiplex ligation-dependent probe amplification analysis was used to investigate for copy number variants. Genotyping of 98 single nucleotide variants allowed allele frequency comparisons with public databases. Seven VWD2 mutations and 36 candidate VWD1 mutations (5 deletions, 4 nonsense, 21 missense, 1 splice, and 5 synonymous mutations) were identified. Nine mutations were found in more than one family and nine VWD1 index cases carried more than one candidate mutation. The T-allele of rs1063857 (c.2385T > C, p.Y795 = ) and blood group O were both frequent findings and contributed to disease in the Swedish VWD1 population. VWD2 mutations were found in 20 and candidate VWD1 mutations in 51 index cases out of 106 (48%). VWF mutations, a VWF haplotype, and blood group O all contributed to explain disease in Swedish VWD1 patients.

  • 54.
    Manderstedt, Eric
    et al.
    Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. Kristianstad University, Faculty of Natural Science, Forskningsmiljön Biomedicin.
    Lind-Halldén, Christina
    Kristianstad University, Faculty of Natural Science, Forskningsmiljön Biomedicin. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Svensson, Peter
    Lund University.
    Zöller, Bengt
    Lund University.
    Halldén, C
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Faculty of Natural Science, Forskningsmiljön Biomedicin.
    Next-generation sequencing of 17 genes associated with venous thromboembolism reveals a deficit of non-synonymous variants in procoagulant genes2019In: Thrombosis and haemostasis, ISSN 2567-689X, Vol. 119, no 9, p. 1441-1450Article in journal (Refereed)
    Abstract [en]

    BACKGROUND:  The heritability of venous thromboembolism (VTE) is only partially explained by variants in 17 previously VTE-associated genes.

    OBJECTIVE:  This article screens for additional rare variants in the 17 genes and investigates the relative contributions of pro- and anticoagulant genes to VTE.

    PATIENTS AND METHODS:  Ninety-six VTE patients from the population-based Malmö Thrombophilia Study were analysed using an AmpliSeq strategy and Ion Torrent sequencing and the variant data were compared with data from public databases.

    RESULTS:  A total of 102 non-synonymous and 76 synonymous variants were identified. Forty-six non-synonymous variants were present in the human gene mutation database. Anticoagulant and procoagulant genes showed 14 and 22 rare non-synonymous variants, respectively. Individual patients showed varying numbers of risk factors; 13 patients had non-synonymous mutations in SERPINC1, PROC and PROS1 genes and 42 had factor V Leiden or prothrombin mutations generating a total of 47 patients with at least one of these risk factors. Ten common VTE-associated variants showed low level enrichments and no correlation to the other risk factors. The enrichment of previously identified risk factors was similar to previous studies. Determination of the nsyn/syn ratio (number of non-synonymous variants per non-synonymous site, nsyn, to the number of synonymous variants per synonymous site, syn) showed, as expected in patients, an increase of non-synonymous relative to synonymous anticoagulant variants compared with controls (nsyn/syn, 0.95 vs. 0.68). In contrast, non-synonymous procoagulant variants (nsyn/syn, 0.31 vs. 0.63) showed a decrease. We suggest that the deficit of non-synonymous variants in procoagulant genes is a novel mechanism contributing to VTE.

  • 55.
    Manderstedt, Eric
    et al.
    Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. Kristianstad University, Faculty of Natural Science, Forskningsmiljön Biomedicin.
    Nilsson, Rosanna
    Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. rosanna.nilsson@hkr.se .
    Lind-Halldén, Christina
    Kristianstad University, Faculty of Natural Science, Forskningsmiljön Biomedicin. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Ljung, Rolf
    Skåne University Hospital.
    Astermark, Jan
    Skåne University Hospital.
    Halldén, Christer
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Faculty of Natural Science, Forskningsmiljön Biomedicin.
    Targeted re-sequencing of F8, F9 and VWF: characterization of Ion Torrent data and clinical implications for mutation screening.2019In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 14, no 4Article in journal (Refereed)
    Abstract [en]

    Mutations are not identified in ~5% of hemophilia A and 10-35% of type 1 VWD patients. The bleeding tendency also varies among patients carrying the same causative mutation, potentially indicating variants in additional genes modifying the phenotype that cannot be identified by routine single-gene analysis. The F8, F9 and VWF genes were analyzed in parallel using an AmpliSeq strategy and Ion Torrent sequencing. Targeting all exonic positions showed an average read depth of >2000X and coverage close to 100% in 24 male patients with known disease-causing mutations. Discrimination between reference alleles and alternative/indel alleles was adequate at a 25% frequency threshold. In F8, F9 and VWF there was an absolute majority of all reference alleles at allele frequencies >95% and the average alternative allele and indel frequencies never reached above 10% and 15%, respectively. In VWF, 4-5 regions showed lower reference allele frequencies; in two regions covered by the pseudogene close to the 25% cut-off for reference alleles. All known mutations, including indels, gross deletions and substitutions, were identified. Additional VWF variants were identified in three hemophilia patients. The presence of additional mutations in 2 out of 16 (12%) randomly selected hemophilia patients indicates a potential mutational contribution that may affect the disease phenotype and counseling in these patients. Parallel identification of disease-causing mutations in all three genes not only confirms the deficiency, but differentiates phenotypic overlaps and allows for correct genetic counseling.

  • 56.
    Mohlin, Camilla
    et al.
    Linnaeus University.
    Delbro, Dick
    Örebro University.
    Kvanta, Anders
    Karolinska Institutet.
    Johansson, Kjell
    Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. kjell.johansson@hkr.se .
    Evaluation of Congo red staining in degenerating porcine photoreceptors in vitro: protective effects by structural and trophic support2018In: Journal of Histochemistry and Cytochemistry, ISSN 0022-1554, E-ISSN 1551-5044, Vol. 66, no 9Article in journal (Refereed)
    Abstract [en]

    Congo red (CR) is a histological stain used for the detection of extracellular amyloids mediating various neurodegenerative diseases. Given that damaged photoreceptors appear to degenerate similarly to other nerve cells, CR staining was evaluated in experimentally injured porcine retina. CR staining appeared mostly as discrete cytosolic deposits with no obvious plaque formation during the investigated time period. Increases of CR labeling coincided temporally with the known accumulation of mislocalized opsins and increases of cell death. Coculture, either with human retinal pigment epithelium (ARPE) or human neural progenitor (ReN) cells, was accompanied by a significant reduction of CR labeling. Of particular interest was the reduction of CR labeling in cone photoreceptors, which are important for the perception of color and fine details and afflicted in age-related macular degeneration (AMD). Electron microscopy revealed inclusions in the inner segment, cell body, and occasionally synaptic terminals of photoreceptor cells in cultured specimens. Closer examinations indicated the presence of different types of inclusions resembling protein aggregates as well as inclusion bodies. The current results indicate that injury-related response resulted in accumulation of CR deposits in photoreceptor cells, and that trophic and/or structural support attenuated this response.

  • 57.
    Nielsen, Frederik Knud
    et al.
    Danmark.
    Hansen, Cecilie Hurup
    Danmark.
    Fey, Jennifer Anna
    Danmark.
    Hansen, Martin
    USA.
    Halling-Sørensen, Bent
    Danmark.
    Björklund, Erland
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. Kristianstad University, Faculty of Natural Science, Research environment MoLab.
    Styrishave, Bjarne
    Danmark.
    Mixture effects of 3 mechanistically different steroidogenic disruptors (prochloraz, genistein, and ketoconazole) in the H295R cell assay2015In: International journal of toxicology, ISSN 1091-5818, E-ISSN 1092-874X, Vol. 34, no 6, p. 534-542Article in journal (Refereed)
    Abstract [en]

    Mixture effects of 3 model endocrine disruptors, prochloraz, ketoconazole, and genistein, on steroidogenesis were tested in the adrenocortical H295R cell line. Seven key steroid hormones (pregnenolone, progesterone, dehydroepiandrosterone, androstenedione, testosterone, estrone, and 17β-estradiol) were analyzed using gas chromatography and tandem mass spectrometry (GC-MS/MS) to investigate the effects throughout the steroidogenic pathway. Current modeling approaches often rely on models assuming compounds acting independently and that the individual effects in some way can be summarized to predict a mixture effect. In H295R cells with an intact steroidogenic pathway, such assumptions may not be feasible. The purpose of this study was therefore to evaluate whether effects of a mixture with differing modes of action followed or deviated from additivity (concentration addition) and whether the H295R cell line was suitable for evaluating mixture toxicity of endocrine disruptors with different modes of action. The compounds were chosen because they interfere with steroidogenesis in different ways. They all individually decrease the concentrations of the main sex steroids downstream but exert different effects upstream in the steroidogenic pathway. Throughout the study, we observed lowest observed effect concentrations of mixtures at levels 2 to 10 times higher than the predicted EC50, strongly indicating antagonistic effects. The results demonstrate that chemical analysis combined with the H295R cell assay is a useful tool also for studying how mixtures of endocrine disruptors with differing modes of action interfere with the steroidogenic pathway and that existing models like concentration addition are insufficient in such cases. Furthermore, for end points where compounds exert opposite effects, no relevant models are available.

  • 58.
    Olsson, Camilla
    et al.
    Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. Kristianstad University, Faculty of Natural Science, Research environment Man & Biosphere Health (MABH).
    Elmberg, Johan
    Kristianstad University, Faculty of Natural Science, Research environment Man & Biosphere Health (MABH). Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Liljebäck, Niklas
    Swedish Association for hunting and wildlife management.
    Kruckenberg, Helmut
    Germany.
    Voslamber, Berend
    Netherlands.
    J.D.M. Muskens, Gerard
    Netherlands.
    Månsson, Johan
    Swedish university of agricultural sciences.
    Long-distance and local movements of greylag geese in present-day agricultural landscapes2018In: 18th Conference of Goose Specialist Group: Conference Abstracts / [ed] Rasa Morkune, Modestas Bruzas, Julius Morkunas, Marine Institute of Klaipeda University , 2018, p. 77-Conference paper (Refereed)
    Abstract [en]

    Recent changes in environmental conditions together with increasing goose populations have completely changed the ballgame for geese in Europe. To better understand their current distribution and foraging patterns, this project will explore how geese utilize the agricultural landscape, with focus on their movements, field selection and foraging patterns. We fitted 199 Greylag geese with neck-collars and 64 with GPS transmitters at 5 locations in Sweden. The tagged geese will be used for studying movement patterns at a field-tofield level. However, the GPS transmitters also deliver data that can be used together with re-sightnings of neck-collared geese to unravel large-scale movement patterns of the Swedish Greylag goose population. Preliminary results from GPS positions received June--November 2017 indicate a varation in migration patterns and wintering grounds, depending on the origin of the geese. Geese breeding and molting in the southern parts of Sweden seem to migrate shorter distances, and have spent most of their time during the autumn months in Denmark, or in the southernmost parts of Sweden, while the geese marked farther north migrated earlier and moved longer distances, with the majority spending the autumn in Germany and the Netherlands.

  • 59.
    Oppong Bekoe, Samuel
    et al.
    Faculty of Health and Medical Sciences, University of Copenhagen.
    Bak, Søren Alex
    Faculty of Health and Medical Sciences, University of Copenhagen.
    Björklund, Erland
    Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. Kristianstad University, Faculty of Natural Science, Research environment MoLab. Faculty of Health and Medical Sciences, University of Copenhagen.
    Krogh, Kristine A.
    Faculty of Health and Medical Sciences, University of Copenhagen.
    Okine, Nathaniel N. N. A.
    Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
    Adosraku, Reimmiel K.
    Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
    Styrishave, Bjarne
    Faculty of Health and Medical Sciences, University of Copenhagen.
    Hansen, Martin
    Faculty of Health and Medical Sciences, University of Copenhagen.
    Determination of thirteen antibiotics in drug products: a new LC-MS/MS tool for screening drug product quality2014In: Analytical Methods, ISSN 1759-9660, E-ISSN 1759-9679, Vol. 6, p. 5847-5855Article in journal (Refereed)
    Abstract [en]

    Poor quality antibiotic medicines in circulation in Sub-Saharan Africa continue to be a burden. Pharmaceutical trade in substandard and counterfeit medicines is on the rise. The chemical quality of antibiotics dispensed in health facilities and recognised drug outlets in Ghana, when compromised, could be a major drawback to efforts made in fighting antibiotic resistance globally. To improve on antibiotic drug quality monitoring, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) methodology, which is capable of quantifying thirteen antibiotics in drug products, was developed and validated in present work. The methodology was applied to various drug products including tablets, capsules, suspensions, syrups, intravenous and injection solutions as well as ear and eye droplets used as essential medicines in a Sub-Saharan country, Ghana.

  • 60.
    Palmberg, Irmeli
    et al.
    Finland.
    Kärkkäinen, Sirpa
    Finland.
    Jeronen, Eila
    Finland.
    Yli-Panula, Eija
    Finland.
    Persson, Christel
    Kristianstad University, Faculty of Education, Research environment Learning in Science and Mathematics (LISMA). Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Nordic student teachers’ views on the most effcient teaching and learning methods for species and species identification2019In: Sustainability, ISSN 2071-1050, E-ISSN 2071-1050Article in journal (Refereed)
    Abstract [en]

    Teachers need knowledge of species and species identification skills for teaching thestructure and function of ecosystems, and the principles of biodiversity and its role in sustainability.The aim of this study is to analyze Nordic student teachers’ views on the most ecient methodsand strategies to teach and learn species and species identification, and to find some trends abouthow well their views are reflected in a species identification test. Student teachers in Finland,Norway, and Sweden (N = 426) answered a questionnaire consisting of fixed and open-endedquestions, and a species identification test. An analysis of variance, Chi-Square, and t-test were usedfor quantitative data and an inductive content analysis for qualitative data. Results showed thatoutdoor teaching and learning methods are more ecient than indoor methods. The majority ofstudent teachers considered outdoor experiential learning with living organisms as the most ecientteaching and learning method. Student teachers who highlighted outdoor experiential learning andoutdoor project work as their most ecient methods received significantly better results in the speciesidentification test than the others. Field trips and fieldwork were emphasized as the most importantsources in schools and universities, while the Internet was the most important source among media.The student teachers underlined teachers’ expertise in the form of in-depth understanding of subjectsand supervising skills for ecient teaching both outdoors and indoors. Therefore, teaching andlearning of species and species identification as the practical part of biodiversity and sustainabilityeducation is emphasized as an integral part of teacher education programs.

  • 61.
    Persson, Christel
    et al.
    Kristianstad University, Faculty of Education, Research environment Learning in Science and Mathematics (LISMA). Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap.
    Åberg, Peter
    Mårtensson, Lennart
    Kristianstad University, Faculty of Natural Science, Research environment Man & Biosphere Health (MABH). Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Pedagogiska utmaningar i det digitala klassrummet2018In: Högskolepedagogisk debatt, ISSN 2000-9216, no 2, p. 26-34Article in journal (Other academic)
  • 62.
    Pöysä, Hannu
    et al.
    Finland.
    Holopainen, Sari
    Finland.
    Elmberg, Johan
    Kristianstad University, Faculty of Natural Science, Research environment Man & Biosphere Health (MABH). Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Gunnarsson, Gunnar
    Kristianstad University, Faculty of Natural Science, Research environment Man & Biosphere Health (MABH). Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Nummi, Petri
    Finland.
    Sjöberg, Kjell
    Swedish University of Agricultural Sciences.
    Changes in species richness and composition of boreal waterbird communities: a comparison between two time periods 25 years apart2019In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 9, no 1Article in journal (Refereed)
    Abstract [en]

    Global measures of biodiversity indicate consistent decline, but trends reported for local communitiesare more varied. Therefore, we need better understanding of mechanisms that drive changes in diversity of local communities and of differences in temporal trends between components of local diversity, such as species richness and species turnover rate. Freshwater ecosystems are vulnerable to multiple stressors, and severe impacts on their biodiversity have been documented. We studied species richness and composition of local boreal waterbird communities in 1990/1991 and 2016 at 58 lakes distributed over six regions in Finland and Sweden. The study lakes represented not only local trophic gradients but also a latitudinal gradient in the boreal biome. While species richness tended to be lower in 2016 than in 1990/1991, species turnover was relatively high. Within foraging guilds, local species richness of diving ducks and surface feeding waterbirds decreased, whereas that of large herbivores increased. The number of species gained in local communities was higher in lakes with rich vegetation than in lakes with sparse vegetation. Conservation of boreal freshwater ecosystems would benefit from recognizing  hat large-scale environmental changes can affect local diversity via processes operating atfiner scales.

  • 63.
    Rehnstam-Holm, Ann-Sofi
    et al.
    Kristianstad University, Faculty of Natural Science, Research environment Man & Biosphere Health (MABH). Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Axelsson, Carolina
    Kristianstad University, Faculty of Natural Science, Research environment Man & Biosphere Health (MABH). Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Forskningsanknuten grundutbildning: utbildningsanknuten forskning?2018In: Man and Biosphere Health: en komplett akademisk miljö / [ed] Ann-Sofi Rehnstam-Holm, Kristianstad: Högskolan Kristianstad , 2018, p. 8-15Chapter in book (Other academic)
    Abstract [sv]

    Begreppet forskningsanknuten utbildning kan definieras på ett flertal sätt. Idealt ska studenterna inom sin utbildning stöta på forskning genom att få kännedom om aktuell forskning och vara praktiskt involverade i forskningsprojekt. För att detta ska uppnås bör majoriteten av lärarna vara forskningsaktiva inom för utbildningen relevanta områden och att ett vetenskapligt förhållningssätt till kunskap förmedlar på bästa pedagogiska sätt också detta förankrat i forskning. I den här artikeln ger vi exempel på hur studenter har involverats i våra forskningsprojekt både på högskolan, på universitetssjukhuset i Lund och vid internationella universitet och forskningsstationer.

  • 64.
    Rodriguez-Navas, Carlos
    et al.
    Danmark.
    Björklund, Erland
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. Kristianstad University, Faculty of Natural Science, Research environment MoLab.
    Halling-Sørensen, Bent
    Danmark.
    Hansen, Martin
    Danmark.
    Biogas final digestive byproduct applied to croplands as fertilizer contains high levels of steroid hormones2013In: Environmental Pollution, ISSN 0269-7491, E-ISSN 1873-6424, Vol. 180, p. 368-371Article in journal (Refereed)
    Abstract [en]

    In this study we evaluate and demonstrate the occurrence of nine natural and one synthetic steroid hormone, including estrogens, androgens and progestagens in biogas final digestate byproduct (digestion liquid) commonly used as an agricultural fertilizer. We investigated two biogas sites that utilize different anaerobic digestion technologies (mesophilic and thermophilic) from swine manure and other organic wastes. Individual hormone concentration levels were observed up to 1478 ng g−1 dry weight or 22.5 mg kg−1 N with estrone and progesterone reaching highest concentration levels. Evaluation of the potential environmental burden through the application in agriculture was also assessed on the basis of predicted environmental concentrations. This study indicates that the biogas digestion process does not completely remove steroid hormones from livestock manure and use of final digestate byproduct on croplands contributes to the environmental emission of hormones.

  • 65.
    Sanagavarapu, Kalyani
    et al.
    Lund University.
    Nüske, Elisabeth
    Germany.
    Nasir, Irem
    USA.
    Meisl, Georg
    England.
    Immink, Jasper N
    Lund University.
    Sormanni, Pietro
    England.
    Vendruscolo, Michele
    England.
    Knowles, Tuomas P J
    England.
    Malmendal, Anders
    Lund University.
    Cabaleiro-Lago, Celia
    Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. Kristianstad University, Faculty of Natural Science, Research environment Man & Biosphere Health (MABH). Lund University.
    Linse, Sara
    Lund University.
    A method of predicting the in vitro fibril formation propensity of Aβ40 mutants based on their inclusion body levels in E. coli2019In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 9, no 1Article in journal (Refereed)
    Abstract [en]

    Overexpression of recombinant proteins in bacteria may lead to their aggregation and deposition in inclusion bodies. Since the conformational properties of proteins in inclusion bodies exhibit many of the characteristics typical of amyloid fibrils. Based on these findings, we hypothesize that the rate at which proteins form amyloid fibrils may be predicted from their propensity to form inclusion bodies. To establish a method based on this concept, we first measured by SDS-PAGE and confocal microscopy the level of inclusion bodies in E. coli cells overexpressing the 40-residue amyloid-beta peptide, Aβ40, wild-type and 24 charge mutants. We then compared these results with a number of existing computational aggregation propensity predictors as well as the rates of aggregation measured in vitro for selected mutants. Our results show a strong correlation between the level of inclusion body formation and aggregation propensity, thus demonstrating the power of this approach and its value in identifying factors modulating aggregation kinetics.

  • 66.
    Styrishave, Bjarne
    et al.
    Danmark.
    Pedersen, Kathrine Eggers
    Danmark.
    Clarke, Olivier
    Danmark.
    Hansen, Martin
    Danmark.
    Björklund, Erland
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. Kristianstad University, Faculty of Natural Science, Research environment MoLab. University of Copenhagen.
    Sonne, Christian
    Danmark.
    Dietz, Rune
    Danmark.
    Steroid hormones in multiple tissues of East Greenland polar bears (Ursus maritimus)2017In: Polar Biology, ISSN 0722-4060, E-ISSN 1432-2056, Vol. 40, no 1, p. 37-49Article in journal (Refereed)
    Abstract [en]

    The polar bear (Ursus maritimus) is threatened by climate changes and also from persistent organic pollutants affecting polar bear endocrinology governing growth and reproduction. To provide further insight into basic polar bear endocrinology, we determined the levels of steroids in multiple tissues and plasma from East Greenland polar bears. Tissue samples from 10 polar bears, 5 males (2 adults, 3 juveniles) and 5 females (all juveniles) were obtained from the Inuit hunt in Scoresby Sound during springtime. Eleven steroids: pregnenolone, 17-hydroxypregnenolone, progesterone, 17-hydroxyprogesterone, dehydroepiandrosterone, androstenedione, testosterone, dihydrotestosterone, estrone, 17 alpha-estradiol and 17 beta-estradiol were determined in brain, adrenal cortex, testis, testicular vein, plasma, and ovary using GC-MS/MS. In brain tissue, the neuroactive progestagen pregnenolone (11.9 +/- 4.4 ng/g ww) and dehydroepiandrosterone (2.26 +/- 0.43 ng/g ww) were found in high concentrations. Very high levels of testosterone and androstenedione were observed in testes (> 100 ng/g ww) and plasma from testicular vein (testosterone: 108 +/- 41 ng/ml; androstenedione: 35.2 +/- 11.1 ng/ml). Additionally, a strong correlation was found between the levels of steroids in testes and testicular vein plasma. Progestagens were found in very high levels in ovaries from juvenile females (> 100 ng/g ww). Finally, our study indicates that polar bears synthesize androstenedione via the a dagger-4 pathway. The present study adds new insight to our knowledge on polar bear endocrinology, which may be used in future studies on polar bear ecology and studies on some of the threats from pollution and climate changes that these animals are facing.

  • 67.
    Subedi, Bikram
    et al.
    Baylor University, Waco, USA.
    Aguilar, Lissette
    Baylor University, Waco, USA.
    Robinson, Eleanor M.
    Baylor University, Waco, USA.
    Hageman, Kimberley J.
    University of Otago, New Zealand.
    Björklund, Erland
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. Kristianstad University, Faculty of Natural Science, Research environment MoLab.
    Sheesley, Rebecca J.
    Baylor University, Waco, USA.
    Usenko, Sascha
    Baylor University, Waco, USA.
    Selective pressurized liquid extraction as a sample-preparation technique for persistent organic pollutants and contaminants of emerging concern2015In: TrAC. Trends in analytical chemistry, ISSN 0165-9936, E-ISSN 1879-3142, Vol. 68, p. 119-132Article in journal (Refereed)
    Abstract [en]

    Sample preparation represents about two-thirds of the cost of analysis and often presents logistical bottlenecks in analytical and environmental chemistry laboratories, so reducing our capacity and preparedness to quantify organic pollutants rapidly and accurately. Selective pressurized liquid extraction (SPLE) is an analytical technique that builds upon PLE by incorporating matrix-compound (i.e., interference) retainers into the extraction step, so reducing sample-preparation steps and increasing sample throughput. SPLE methods offer distinct advantages over traditional methods, namely reduction in the costs intrinsic to sample preparation (i.e., time, solvents, labor, laboratory space, training, and potential loss of analytes). The ability to analyze and to evaluate rapidly a large number of samples directly increases the analytical capacity and preparedness of a laboratory for certain situations (e.g., large-scale studies or environmental emergencies). We review the analytical improvements via SPLE and its wide-ranging applications.

  • 68.
    Svahn, Ola
    et al.
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Research environment MoLab.
    Björklund, Erland
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. Kristianstad University, Faculty of Natural Science, Research environment MoLab.
    Describing sorption of pharmaceuticals to lake and river sediments, and sewage sludge from UNESCO Biosphere Reserve Kristianstads Vattenrike by chromatographic asymmetry factors and recovery measurements2015In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 415, p. 73-82Article in journal (Refereed)
    Abstract [en]

    Over the past 30 years a vast number of studies have demonstrated the presence of pharmaceutical residues in the environment. But still knowledge is scarce regarding the interaction of these emerging pollutants with various matrices in nature. A chromatographic system with on-line detection was developed to perform a sorption study of six selected pharmaceuticals to four natural sediments and dewatered digested sewage treatment plant sludge with differing physicochemical characteristics. Sorption effects, measured as asymmetry factors and recoveries, differed pronouncedly among the pharmaceuticals and between the matrices, which could be explained by basic physicochemical properties of the investigated compounds in relation to matrix characteristics. Protonated and deprotonated molecular properties had the greatest importance for sorbate–sorbent interactions. Atenolol, with cationic properties, showed the highest degree of sorption regardless of the matrix studied. Diclofenac and furosemide, both acids, showed the least tendency towards interactions to natural matrices. Among the neutral compounds bendroflumethiazide, carbamazepine and oxazepam, weaker forces, such as van der Waals, aromatic electron donor–acceptor interactions, and hydrogen forces, seemed more important to determine sorption differences. Results revealed that sorption of pharmaceuticals on natural sediments decreased in the order: atenolol (+) > bendroflumethiazide > oxazepam > carbamazepine > diclofenac (−) > furosemide(–). The matrix content of organic matter measured as total organic carbon (TOC) clearly dictated drug sorption. Beside from studying matrix interaction, these results and the developed technique and methodology might find use in the development of new removal processes of pharmaceuticals from wastewater based on improved knowledge concerning chemical interactions to filter materials.

  • 69.
    Svahn, Ola
    et al.
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Research environment MoLab.
    Björklund, Erland
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. Kristianstad University, Faculty of Natural Science, Research environment MoLab.
    Extraction Efficiency of a Commercial Espresso Machine Compared to a Stainless-Steel Column Pressurized Hot Water Extraction (PHWE) System for the Determination of 23 Pharmaceuticals, Antibiotics and Hormones in Sewage Sludge2019In: Applied Sciences, E-ISSN 2076-3417, Vol. 9, no 7, article id 1509Article in journal (Refereed)
    Abstract [en]

    Two green chemistry extraction systems, an in-house stainless-steel column Pressurized Hot Water Extraction system (PHWE) and a commercially available Espresso machine were applied for analysing 23 active pharmaceutical ingredients (APIs) in sewage sludge. Final analysis was performed on UPLC-MS/MS using two different chromatographic methods: acid and basic. When analysing all 23 APIs in sewage sludge both extraction methods showed good repeatability. The PHWE method allowed for a more complete extraction of APIs that were more tightly bound to the matrix, as exemplified by much higher concentrations of e.g., ketoconazole, citalopram and ciprofloxacin. In total, 19 out of 23 investigated APIs were quantified in sewage sludge, and with a few exceptions the PHWE method was more exhaustive. Mean absolute recoveries of 7 spiked labelled APIs were lower for the PHWE method than the Espresso method. Under acid chromatographic conditions mean recoveries were 16% and 24%, respectively, but increased to 24% and 37% under basic conditions. The difference between the PHWE method and the Espresso method might be interpreted as the Espresso method giving higher extraction efficiency; however, TIC scans of extracts revealed a much higher matrix co-extraction for the PHWE method. Attempts were made to correlate occurrence of compounds in sewage sludge with chemical properties of the 23 APIs and there are strong indications that both the number of aromatic rings and the presence of a positive charge is important for the sorption processes to sewage sludge.

  • 70.
    Svahn, Ola
    et al.
    Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. Kristianstad University, Faculty of Natural Science, Research environment MoLab.
    Björklund, Erland
    Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. Kristianstad University, Faculty of Natural Science, Research environment MoLab.
    High flow-rate sample loading in large volume whole water organic trace analysis using positive pressure and finely ground sand as a SPE-column in-line filter2019In: Molecules, ISSN 1420-3049, E-ISSN 1420-3049, Vol. 24, no 7Article in journal (Refereed)
    Abstract [en]

    By using an innovative, positive pressure sample loading technique in combination with an in-line filter of finely ground sand the bottleneck of solid phase extraction (SPE) can be reduced. Recently published work by us has shown the proof of concept of the technique. In this work, emphasis is put on the SPE flow rate and method validation for 26 compounds of emerging environmental concern, mainly from the 1st and 2nd EU Watch List, with various physicochemical properties. The mean absolute recoveries in % and relative standard deviations (RSD) in % for the investigated compounds from spiked pure water samples at the three investigated flow rates of 10, 20, and 40 mL/min were 63.2% (3.2%), 66.9% (3.3%), and 69.0% (4.0%), respectively. All three flow rates produced highly repeatable results, and this allowed a flow rate increase of up to 40 mL/min for a 200 mg, 6 mL, reversed phase SPE cartridge without compromising the recoveries. This figure is more than four times the maximum flow rate recommended by manufacturers. It was indicated that some compounds, especially pronounced for the investigated macrolide molecules, might suffer when long contact times with the sample glass bottle occurs. A reduced contact time somewhat decreases this complication. A very good repeatability also held true for experiments on both spiked matrix-rich pond water (high and low concentrations) and recipient waters (river and wastewater) applying 40 mL/min. This work has shown that, for a large number of compounds of widely differing physicochemical properties, there is a generous flow rate window from 10 to 40 mL/min where sample loading can be conducted. A sample volume of 0.5 L, which at the recommended maximum flow rate speed of 10 mL/min, would previously take 50 min, can now be processed in 12 min using a flow rate of 40 mL/min. This saves 38 min per processed sample. This low-cost technology allows the sample to be transferred to the SPE-column, closer to the sample location and by the person taking the sample. This further means that only the sample cartridge would need to be sent to the laboratory, instead of the whole water sample, like today's procedure.

  • 71.
    Svahn, Ola
    et al.
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Research environment MoLab.
    Björklund, Erland
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. Kristianstad University, Faculty of Natural Science, Research environment MoLab.
    Increased electrospray ionization intensities and expanded chromatographic possibilities for emerging contaminants using mobile phases of different pH2016In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 1033-1034, p. 128-137Article in journal (Refereed)
    Abstract [en]

    In this work the habitual behaviour of low pH in environmental organic trace analysis is challenged by investigating the full potential of building a multi-component UHPLC-ESI-MS/MS method adapted to cover common emerging contaminants of many different polarities, minimizing the elements of compromise in the performance of the final analytical separation and detection. Contributes have been made by taking advantage of common commercially available technology in understanding the impact from solvent components and the ionization of analytes which can facilitate future development of robust, sensitive and precise UHPLC-MS/MS methods. All contaminants were evaluated and optimized without prejudices regarding historical residence in terms of chromatographic conditions and ESI mode; increasing multi-method's flexibility that can be implemented in routine analysis in response to new requests as well as to emerging contaminants yet to be discovered. Our data strongly supports the questioning of the assumption that equilibrium concentrations of ions in solution reflect those produced during the electrospray process. ESI responses of [M+H](+) and limits of detection were comparable, or often better at high pH compared to acidic eluents. Presence of nitrogen basic groups such as tertiary and secondary amines in a compound increased the intensity of the ESI+ signal, and was even further elevated in basic eluent. The proton affinity probably changes for many nitrogen-containing compounds during the ionization process, making the gas-phase processes very important in generation of these ions by ESI+. There were also an unexpected large number of compounds showing their highest response at pH 7 and weak ionic strength. A flow optimized, buffert free, neutral UHPLC-MS/MS method enhanced the sensitivity for the environmental important synthetic hormone ethinyl estradiol significantly.

  • 72.
    Svahn, Ola
    et al.
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Research environment MoLab.
    Björklund, Erland
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. Kristianstad University, Faculty of Natural Science, Research environment MoLab.
    Interkalibrerad läkemedelsanalys 2017: ett samarbetsprojekt för ökad analyskvalité2017Report (Other academic)
    Abstract [sv]

    Läkemedelsverket gav i september 2015 ut en rapport med titeln ”Miljöindikatorer inom ramen för nationella läkemedelsstrategin (NLS)”. Rapporten indikerar bland annat stora kvalitetsbrister i tidigare utförda analyser av läkemedel i miljön under åren 2002-2013, men betonar också vikten av att samordna framtida nationella läkemedelsanalyser för att bättre använda våra offentliga resurser. I rapporten skriver man följande: ”Allra högst prioriterad ansåg arbetsgruppen att indikatorn ”mäta halter av läkemedelssubstanser i miljö” vara. Detta beroende på att det utöver att det är av stor vikt att följa utvecklingen av läkemedelsrester i miljön över tid för att utvärdera effekten av genomförda insatser, så ansåg arbetsgruppen att det finns stora möjligheter att optimera användningen av de offentliga resurserna genom en bättre samordning av mätningar i miljön. Många mätningar har gjorts historiskt av olika offentliga aktörer utan någon samordning.” Mot bakgrund av detta, och som svar på ett nationellt behov, åtog sig Högskolan Kristianstad (HKR) genom Ola Svahn och Erland Björklund, båda verksamma vid MoLab, Krinova Incubator and Science Park i Kristianstad, att leda denna interkalibreringsstudie för läkemedelsanalys i miljö-vattenprover av olika typ. Arbetet har skett på uppdrag av Havs- och Vattenmyndigheten som finansierat arbetet via bidrag ur havs- och vattenmiljöanslaget och i samarbete med fyra andra analyslaboratorier vid Umeå Universitet (UU), Sveriges Lantbruksuniversitet Uppsala (SLU), Svenska miljöinstitutet Stockholm (IVL) samt Aarhus Universitet Danmark (AU). Total deltog därmed 5 laboratorier, alla med tidigare erfarenhet av läkemedelsanalys i miljöprover.

  • 73.
    Svahn, Ola
    et al.
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Research environment MoLab.
    Björklund, Erland
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. Kristianstad University, Faculty of Natural Science, Research environment MoLab.
    Läkemedelsutsläpp från Skånska avloppsreningsverk 2017: ett uvecklings- och samarbetsprojekt på Högskolan Kristianstad i samarbete med Region Skåne och 6 skånska reningsverksaktörer2017Report (Other academic)
    Abstract [sv]

    I projektet LUSKA (Läkemedelsutsläpp från Skånska Avloppsreningsverk) deltog 6 olika reningsverksorganisationer,    geografiskt fördelade över hela Skåne; Höganäs, Klippan, Höör/Hörby, Svedala, Kristianstad och Simrishamn. Provtagning utfördes i april 2017 på fyra platser vid varje reningsverk. Tre av dessa platser valdes enligt förslag från Länsstyrelsen Skånes tillsynsvägledning; uppströms, nedströms och utgående vatten från reningsverket. Dessutom ingick en fjärde provpunkt bestående av inkommande vatten till reningsverken. I studien analyserades total 21 av 22 läkemedel enligt Läkemedelsverkets föreslagna lista på ämnen från 2015. Analysresultaten från de 8 avloppsreningsverken, samt tillhörande recipient i form av åar och sjöar visar tydligt att stora mängder läkemedel hamnar i våra omgivande skånska vatten varje år. Detta sker som en konsekvens av att reningsverken inte förmår rena läkemedelsutsläpp med befintlig teknik baserad på aktivt slam. Studien visade att de 8 reningsverken släpper ut minst 71 kg läkemedel varje år till skånska vatten enbart av dessa 21 ämnen. Den stora bulken av de uppmätta ämnena utgjordes av blodtryckssänkaren metoprolol och smärtstillaren diklofenak. Men även ämnen som karbamazepin, losartan, naproxen och oxazepam förekom i betydande koncentrationer i avloppsvattnet. Dessa ämnen innefattar flera läkemedelstyper och representerar tre generella och relativt vanliga sjukdomstillstånd som högt blodtryck, inflammation och smärta, samt depression och ångest. Baserat på resultaten i LUSKA-projektet kan man uppskatta att när ett reningsverk behandlar en miljon kubikmeter (1 000 000 m3) avloppsvatten passerar det samtidigt ut ca 4 kg av de 21 läkemedel som Läkemedelsverket tagit upp på sin övervakningslista. Enligt en grov beräkning, innefattande en majoritet av de skånska reningsverken, skulle detta innebära att nästan 600 kg kilo läkemedel läcker ut varje år från skånska reningsverk av Läkemedelsverkets föreslagna ämnen. Samtidigt måste man beakta att dessa 21 ämnen endast utgör en liten del av flera hundra läkemedelssubstanser som används för behandling av sjukdomar. Med stor sannolikhet läcker ett till flera ton läkemedel ut årligen i skånsk recipient. Mätningar i åar och sjöar visar att även om koncentrationerna sjunker nedströms verken som  en konsekvens av företrädesvis utspädning så finns det lokaler där koncentrationerna är an-märkningsvärt höga utifrån ett hållbarhetsperspektiv.

  • 74.
    Svahn, Ola
    et al.
    Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. Kristianstad University, Faculty of Natural Science, Research environment MoLab.
    Björklund, Erland
    Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. Kristianstad University, Faculty of Natural Science, Research environment MoLab.
    Simple, fast and inexpensive large "whole water" volume sample SPE-loading using compressed air and finely ground sand2019In: Analytical Methods, ISSN 1759-9660, E-ISSN 1759-9679, Vol. 11, no 7, p. 894-896Article in journal (Refereed)
    Abstract [en]

    In environmental trace analysis there is often a need to enrich the compounds of interest from a large sample volume, where the use of solid phase extraction (SPE) is more or less the standard technique. The presence of the sample matrix can cause clogging of the SPE-column, especially at the end of a sample load. Swedish surface waters are often humic rich making the use of traditional sample loading by a vacuum manifold very limited. This obstacle forced the development of a different sample loading technique, based on compressed air and sand as an in-line-filter, designed to load larger sample volumes as needed in trace level analysis of hormones in surface water. The developed technique, combined with a UPLC MS/MS method, showed promising reproducibility and accuracy, and enabled increased sensitivity for the analysis of hormones in humic rich surface water.

  • 75.
    Svahn, Ola
    et al.
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Research environment MoLab.
    Björklund, Erland
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. Kristianstad University, Faculty of Natural Science, Research environment MoLab.
    Thermal stability assessment of antibiotics in moderate temperature and subcriticalwater using a pressurized dynamic flow-through system2015In: International Journal of Innovation and Applied Studies, ISSN 2028-9324, Vol. 11, no 4, p. 872-880Article in journal (Refereed)
    Abstract [en]

    Thermal degradation of antibiotics has been studied for decades in a broad range of disciplines including food production, agriculture and analytical chemistry. Yet, there is a lack of thermal stability data for many antibiotics. Here we systematically investigated the thermal stability of ten commonly prescribed antibiotics applying a laborsaving automated inhouse pressurized dynamic flow-through system. The design of the system allowed a fast access to a large number of data at medium to subcritical water temperatures, ranging from 50-250 °C. The five ß-lactams cefadroxil, cefuroxime, amoxicillin, penicillin V, and penicillin G showed a high degree of stability with a maximum degradation of less than 30 % at 150 °C. The two quinolones ciprofloxacin and norfloxacin showed a very high thermal stability up to 200 °C, as did trimethoprim andsulfamethoxazole. At 250 °C all antibiotics were either partly of fully removed. The tetracycline doxycycline showed a specific removal pattern probably involving both binding to metal surfaces at lower temperatures as well as degradation at increased temperatures.

  • 76.
    Söderquist, Pär
    Kristianstad University, Faculty of Natural Science, Research environment Man & Biosphere Health (MABH). Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Large-scale releases of native species: the mallard as a predictive model system2015Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    Human alteration of natural systems, and its consequences are of great concern and the impact on global ecosystems is one of the biggest threats that biodiversity stands before. Translocations of invasive species, as well as intraspecific contingents with non-native genotypes, whether they are deliberate or unintentional, are one such alteration and its consequences are continuously being assessed. The mallard (Anas platyrhynchos) is the most numerous and widespread duck in the world and a flagship in wetland conservation. It is also an important game species which is heavily restocked for hunting purposes, especially in Europe where over three million ducklings are released every year. Because of its hunted status, its abundance, and the number of released individuals, it can serve as a model species to study effects of releases, both for conservation and restocking for hunting, on wild populations. In this thesis the status of the mallard was assessed in the Nordic countries and the effects of releases on the wild populations were studied by mining historical ringing data, comparing morphology of present-day wild, farmed, and historical mallards, and analyzing phylogeography of wild and farmed mallards in Europe. The status of the mallard population in the Nordic countries are generally good, however, a joint effort of European countries is needed to monitor and manage the population. A significant difference between wild and farmed mallards concerning longevity, migration, bill morphology and genetic structure was also found, together with signs of cryptic introgression of farmed genotypes in the wild population with potential fitness reduction as a result. The effect is however limited by that only a fraction of released farmed mallards reach the breeding season due to low survival. A natural captive environment is crucial to keep individuals wild-like with high survival rates after release. However, with an introgression of potentially maladapted farmed genotypes leading to a reduction in fitness, a low survival of released mallards would favor the wild population. A legislative change regarding obligation to report numbers, provenance, and release sites of farmed mallard should be considered, together with practical solutions of ringing and genetic monitoring of released mallards.

  • 77.
    Söderquist, Pär
    et al.
    Kristianstad University, Faculty of Natural Science, Research environment Man & Biosphere Health (MABH). Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Elmberg, Johan
    Kristianstad University, Faculty of Natural Science, Research environment Man & Biosphere Health (MABH). Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Gunnarsson, Gunnar
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Faculty of Natural Science, Research environment Man & Biosphere Health (MABH).
    Effekter av utsättningar av farmade änder ämnade för jakt2018In: Man and Biosphere Health –: en komplett akademisk miljö / [ed] Rehnstam-Holm, A.-S., Kristianstad: Högskolan Kristianstad , 2018, p. 41-47Chapter in book (Other academic)
    Abstract [sv]

    I över 40 år har det i Sverige pågått ett storskaligt, ekologiskt experiment där hundratusentals gräsänder årligen har fötts upp i fångenskap och satts ut i våtmarker för att öka på den jaktbara populationen. Genom uppfödning i fångenskap riskerar man att de farmade gräsänderna blir annorlunda gentemot de vilda. För att studera effekterna på den vilda populationen av dessa utsättningar startades 2010 ett doktorandprojekt finansierat av Naturvårdsverket. Projektet kom fram till att farmade änder skiljer sig i beteende och utseende mot de vilda. Det går även att genetiskt skilja på farmade och vilda gräsänder. Ett nytt projekt startades 2017 för att vidare studera effekter av utsättningar, nu även på miljön och den biologiska mångfalden där änderna sätts ut. Projekten om effekter av utsättningar av farmade änder involverar forskare från olika lärosäten i Sverige, men också från flera olika europeiska länder. Ett nära samarbete med olika lokala privata aktörer som uppfödare, markägare, jägare och viltmästare samt större organisationer somViltmästareförbundet och Svenska Jägareförbundet är avgörande för projektens framgång. Att kommuniceraresultaten på så väl vetenskapliga konferenser som för icke akademiker involverade i projekten samt i undervisning på Högskolan Kristianstad och på gymnasieskolor har hela tiden varit en målsättning. Projekten har också en tydlig plats i forskningsmiljön MABH vars kompetenser inom t.ex. vattenvård, sjukdomsspridning och landskapsutnyttjande kanknytas till projekten.

  • 78.
    Weisser, Johan J.
    et al.
    Danmark.
    Hansen, Martin
    Danmark.
    Björklund, Erland
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. Kristianstad University, Faculty of Natural Science, Research environment MoLab.
    Sonne, Christian
    Danmark.
    Dietz, Rune
    Danmark.
    Styrishave, Bjarne
    Danmark.
    A novel method for analysing key corticosteroids in polar bear (Ursus maritimus) hair using liquid chromatography tandem mass spectrometry2016In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 1017, p. 45-51Article in journal (Refereed)
    Abstract [en]

    This paper presents the development and evaluation of a methodology for extraction, clean-up and analysis of three key corticosteroids (aldosterone, cortisol and corticosterone) in polar bear hair. Such a methodology can be used to monitor stress biomarkers in polar bears and may provide as a useful tool for long-term and retrospective information. We developed a combined pressurized liquid extraction (PLE)-solid phase extraction (SPE) procedure for corticosteroid extraction and clean-up followed by high pressure liquid chromatography tandem mass spectrometry (HPLC-MS/MS) analysis. This procedure allows for the simultaneous determination of multiple steroids, which is in contrast to previous polar bear studies based on ELISA techniques. Absolute method recoveries were 81%, 75% and 60% for cortisol, corticosterone and aldosterone, respectively. We applied the developed method on a hair sample pooled from four East Greenland polar bears. Herein cortisol and corticosterone were successfully determined in levels of 0.32 +/- 0.02 ng/g hair and 0.13 +/- 0.02 ng/g hair, respectively. Aldosterone was below limit of detection (LOD<0.17 ng/g). The cortisol hair concentration found in these East Greenland polar bears was consistent with cortisol levels previously determined in the Southern Hudson Bay and James Bay in Canada using ELISA kits. (C) 2016 Elsevier B.V. All rights reserved.

  • 79.
    Wendin, Karin
    et al.
    Kristianstad University, Faculty of Natural Science, Research Environment Food and Meals in Everyday Life (MEAL). Kristianstad University, Faculty of Natural Science, Avdelningen för mat- och måltidsvetenskap.
    Mårtensson, Lennart
    Kristianstad University, Faculty of Natural Science, Research environment Man & Biosphere Health (MABH). Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Sensory quality of drinking water in relation to chemical and microbiological composition2019In: 13th Pangborn Sensory Scinece Symposium, 2019Conference paper (Refereed)
    Abstract [en]

    It is well known that tap water tastes different due to where the water is tapped, ie its chemical and microbiological compositions. Taste competitions have been performed in different countries to find out the most preferred tap water. With some few exceptions, no studies have been performed in which the taste of tap water is objectively described by analytical sensory methods. The purpose of this study was to evaluate quality of Swedish drinking water from different dwells with emphasis on sensory, chemical and microbiological analyses.

     

    Tap water origin from surface water and from groundwater was collected from the Swedish municipalities Svalöv and Kristianstad. The water samples were collected in 3 points in each municipality: 1. waterworks; 2. near waterworks; 3. far from waterworks. In addition the commercial water Evian was included in the analyses. Analytical sensory analyses (triangle test and quantitative descriptive analysis) were performed along with chemical and microbiological standard analyses according to Swedish drinking water standards.

     

    The results from the triangle test showed significant sensory differences between tap water from surface water and groundwater, as well as in surface water samples collected in different points. The descriptive analysis showed large differences in the perception of bitterness, minerals and off flavours. The perceived differences are in line with earlier studies pointing out inorganic ions as responsible for the taste sensations. The chemical analyses showed that the major difference between the two types of tap water was a higher mineral content and higher alkalinity in the groundwater compared to the surface water. The microbiological analyses did not show any measurable concentrations in any of the samples. There is a need for further research of chemical and microbiological effects on the flavour of tap water, with focus on microorganisms and chemical compounds in low concentrations.

  • 80.
    Wendin, Karin
    et al.
    Kristianstad University, Faculty of Natural Science, Research Environment Food and Meals in Everyday Life (MEAL). Kristianstad University, Faculty of Natural Science, Avdelningen för mat- och måltidsvetenskap.
    Olsson, Viktoria
    Kristianstad University, Faculty of Natural Science, Research Environment Food and Meals in Everyday Life (MEAL). Kristianstad University, Faculty of Natural Science, Avdelningen för mat- och måltidsvetenskap.
    Forsberg, Sarah
    Kristianstad University, School of Education and Environment, Avdelningen för Mat- och måltidsvetenskap. Kristianstad University, Faculty of Natural Science, Research Environment Food and Meals in Everyday Life (MEAL).
    Gerberich, Johanna
    Kristianstad University, Faculty of Natural Science, Avdelningen för mat- och måltidsvetenskap. Kristianstad University, Faculty of Natural Science, Research Environment Food and Meals in Everyday Life (MEAL).
    Birch, Karina
    Kristianstad University, Faculty of Natural Science, Avdelningen för mat- och måltidsvetenskap. karina.birch@hkr.se .
    Berg, Johan
    RISE.
    Langton, Maud
    SLU.
    Davidsson, Fredrik
    Geoloc.
    Stuffe, Sofia
    Atria.
    Andersson, Peter
    Solina Group.
    Rask, Susanne
    Solina Group.
    Cedergardh, Fanny
    TetraPak.
    Jönsson, K. Ingemar
    Kristianstad University, Faculty of Natural Science, Research environment Man & Biosphere Health (MABH). Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Insects as food: a pilot study for industrial production2019In: 13th Pangborn Sensory Science Symposium, 2019Conference paper (Refereed)
    Abstract [en]

    Despite the many papers reporting on disgust factors of eating insects in Western cultures, the interest of insects as food is increasing, not least because they are nutritious, sustainable and tasty! The time has come to take the next step by making insects available not only as delicious restaurant food, but also for industrial production of foods and meals based on insects. The sensory attributes are of greatest importance to increase understanding of insects as a main ingredient in production and shelf life.

     

    By the use of factorial designs with mealworms as main ingredient, the aim was to evaluate the sensory impact of additions such as salt, oil/water and antioxidant agent. Also the impact of particle size of the mealworms was evaluated.

    Cooked fresh mealworms cut or ground into different particle sizes, oil, water, salt and rosemary were blended according to a factorial design. The resulting products were evaluated by descriptive sensory analysis in addition to instrumental measurements of viscosity and colour. Nutritional contents were calculated. 

    Results showed that particle size of the mealworms had a great impact, ie an increased particle size increased the yellowness and the perceived coarseness. Further, both viscosity and crispiness increased. An increased particle size also meant a decreased odour, probably due to decreased exposure of particle surface. Increased salt content did, as expected, increase saltiness. It also increased the nutty flavour, probably due to the polarity of Sodium Chloride. Different ratios of oil/water did not seem to impact the sensory properties. With reference to the anti-oxidative effects of carnosic acid and carnosol, addition of rosemary had a significant impact on shelf life in terms of decreased rancidity and colour changes. All samples were high in protein content.

     

    All factors, but especially particle size of the mealworm fraction, influenced the sensory attributes.

  • 81.
    Wendin, Karin
    et al.
    Kristianstad University, Faculty of Natural Science, Research Environment Food and Meals in Everyday Life (MEAL). Kristianstad University, Faculty of Natural Science, Avdelningen för mat- och måltidsvetenskap.
    Olsson, Viktoria
    Kristianstad University, Faculty of Natural Science, Research Environment Food and Meals in Everyday Life (MEAL).
    Forsberg, Sarah
    Kristianstad University, School of Education and Environment, Avdelningen för Mat- och måltidsvetenskap. Kristianstad University, Faculty of Natural Science, Research Environment Food and Meals in Everyday Life (MEAL).
    Gerberich, Johanna
    Kristianstad University, Faculty of Natural Science, Avdelningen för mat- och måltidsvetenskap. Kristianstad University, Faculty of Natural Science, Research Environment Food and Meals in Everyday Life (MEAL).
    Birch, Karina
    Kristianstad University, Faculty of Natural Science, Avdelningen för mat- och måltidsvetenskap.
    Berg, Johan
    RISE.
    Langton, Maud
    SLU.
    Davidsson, Fredrik
    Geoloc AB.
    Stuffe, Sofia
    Atria Skandinavia.
    Andersson, Peter
    Solina Group.
    Rask, Susanne
    Solina Group.
    Cedergårdh, Fanny
    TetraPak.
    Jönsson, K. Ingemar
    Kristianstad University, Faculty of Natural Science, Research environment Man & Biosphere Health (MABH). Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Insects as food: a pilot study for industrial production2019In: Book of Abstracts of the EAAP 70th Annual  Meeting of the European Federation of Animal Science: Animal Farming for a Healthy World, Ghent, 2019, p. -161Conference paper (Refereed)
    Abstract [en]

    Despite the many papers reporting on disgust factors of eating insects in Western cultures, the interest of insects as food is increasing, not least because they are nutritious, sustainable and tasty! The time has come to take the next step by making insects available not only as delicious restaurant food, but also for industrial production of foods and meals based on insects. The sensory attributes are of greatest importance to increase understanding of insects as a main ingredient in production and shelf life. 

    By the use of factorial designs with mealworms as main ingredient, the aim was to evaluate the sensory impact of additions such as salt, oil/water and antioxidant agent. Also the impact of particle size of the mealworms was evaluated.

    Cooked fresh mealworms cut or ground into different particle sizes, oil, water, salt and rosemary were blended according to a factorial design. The resulting products were evaluated by descriptive sensory analysis in addition to instrumental measurements of viscosity and colour. Nutritional contents were calculated. 

    Results showed that particle size of the mealworms had a great impact, ie an increased particle size increased the yellowness and the perceived coarseness. Further, both viscosity and crispiness increased. An increased particle size also meant a decreased odour, probably due to decreased exposure of particle surface. Increased salt content did, as expected, increase saltiness. It also increased the nutty flavour, probably due to the polarity of Sodium Chloride. Different ratios of oil/water did not seem to impact the sensory properties. With reference to the anti-oxidative effects of carnosic acid and carnosol, addition of rosemary had a significant impact on shelf life in terms of decreased rancidity and colour changes. All samples were high in protein content.

     

    All factors, but especially particle size of the mealworm fraction, influenced the sensory attributes.

12 51 - 81 of 81
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