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  • 51.
    Arzel, Céline
    et al.
    Kristianstad University, Department of Mathematics and Science.
    Stervander, Martin
    Gunnarsson, Gunnar
    Kristianstad University, Department of Mathematics and Science.
    Simändernas ekologi studeras närmare2003In: Vår fågelvärld, ISSN 0042-2649, Vol. 62, no 4, p. 25-Article in journal (Other academic)
  • 52. Arzél, Céline
    et al.
    Elmberg, Johan
    Kristianstad University, School of Education and Environment.
    Guillemain, Matthieu
    Comportement et alimentation de la Sarcelle d'hiver Anas crecca lors de la migration prénuptial2010In: Ornithos: revue d'ornithologie de terrain, ISSN 1254-2962, Vol. 17, no 5, p. 307-315Article in journal (Other academic)
  • 53.
    Asplund, Maria E.
    et al.
    Department of Biological and Environmental Sciences, University of Gothenburg.
    Baden, Susanne P.
    Department of Biological and Environmental Sciences, University of Gothenburg.
    Russ, Sarah
    Department of Biological and Environmental Sciences, University of Gothenburg.
    Ellis, Robert P.
    College of Life and Environmental Sciences, University of Exeter.
    Gong, Ningping
    Department of Biological and Environmental Sciences, University of Gothenburg.
    Hernroth, Bodil E.
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap.
    Ocean acidification and host–pathogen interactions: blue mussels, Mytilus edulis, encountering Vibrio tubiashii2014In: Environmental Microbiology, ISSN 1462-2912, E-ISSN 1462-2920, Vol. 16, no 4, p. 1029-1039Article in journal (Refereed)
    Abstract [en]

    Ocean acidification (OA) can shift the ecological balance between interacting organisms. In this study, we have used a model system to illustrate the interaction between a calcifying host organism, the blue mussel Mytilus edulis and a common bivalve bacterial pathogen, Vibrio tubiashii, with organisms being exposed to a level of acidification projected to occur by the end of the 21st century. OA exposures of the mussels were carried out in relative long-term (4 months) and short-term (4 days) experiments. We found no effect of OA on the culturability of V. tubiashii, in broth or in seawater. OA inhibited mussel shell growth and impaired crystalline shell structures but did not appear to affect mussel immune parameters (i.e haemocyte counts and phagocytotic capacity). Despite no evident impact on host immunity or growth and virulence of the pathogen, V. tubiashii was clearly more successful in infecting mussels exposed to long-term OA compared to those maintained under ambient conditions. Moreover, OA exposed V. tubiashii increased their viability when exposed to haemocytes of OA-treated mussel. Our findings suggest that even though host organisms may have the capacity to cope with periods of OA, these conditions may alter the outcome of host–pathogen interactions, favouring the success of the latter.

  • 54.
    Asplund, Maria E.
    et al.
    Department of Marine Ecology, University of Gothenburg.
    Rehnstam-Holm, Ann-Sofi
    Kristianstad University, School of Education and Environment.
    Atnur, Vijay
    Department of Fishery Microbiology, College of Fisheries, Karnataka Veterinary Animal and Fisheries Sciences University, Bidar, Karnataka, India.
    Raghunath, Pendru
    Department of Fishery Microbiology, College of Fisheries, Karnataka Veterinary Animal and Fisheries Sciences University, Bidar, Karnataka, India.
    Saravanan, Vasudevan
    Department of Fishery Microbiology, College of Fisheries, Karnataka Veterinary Animal and Fisheries Sciences University, Bidar, Karnataka, India.
    Härnstrom, Karolina
    Department of Marine Ecology, University of Gothenburg.
    Collin, Betty
    Kristianstad University, School of Education and Environment.
    Karunasagar, Indrani
    Department of Fishery Microbiology, College of Fisheries, Karnataka Veterinary Animal and Fisheries Sciences University, Bidar, Karnataka, India.
    Godhe, Anna
    Department of Marine Ecology, University of Gothenburg.
    Water column dynamics of Vibrio in relation to phytoplankton community composition and environmental conditions in a tropical coastal area2011In: Environmental Microbiology, ISSN 1462-2912, E-ISSN 1462-2920, Vol. 13, no 10, p. 2738-2751Article in journal (Refereed)
    Abstract [en]

    Vibrio abundance generally displays seasonal patterns. In temperate coastal areas, temperature and salinity influence Vibrio growth, whereas in tropical areas this pattern is not obvious. The present study assessed the dynamics of Vibrio in the Arabian Sea, 1-2 km off Mangalore on the south-west coast of India, during temporally separated periods. The two sampling periods were signified by oligotrophic conditions, and stable temperatures and salinity. Vibrio abundance was estimated by culture-independent techniques in relation to phytoplankton community composition and environmental variables. The results showed that the Vibrio density during December 2007 was 10- to 100-fold higher compared with the February-March 2008 period. High Vibrio abundance in December coincided with a diatom-dominated phytoplankton assemblage. A partial least squares (PLS) regression model indicated that diatom biomass was the primary predictor variable. Low nutrient levels suggested high water column turnover rate, which bacteria compensated for by using organic molecules leaking from phytoplankton. The abundance of potential Vibrio predators was low during both sampling periods; therefore it is suggested that resource supply from primary producers is more important than top-down control by predators.

  • 55.
    Assarsson, A.
    et al.
    Lund University.
    Nasir, I.
    Lund University.
    Lundqvist, M.
    Lund University.
    Cabaleiro-Lago, Celia
    Lund Univeristy.
    Kinetic and thermodynamic study of the interactions between human carbonic anhydrase variants and polystyrene nanoparticles of different size2016In: RSC Advances, ISSN 2046-2069, E-ISSN 2046-2069, Vol. 6, no 42, p. 35868-35874Article in journal (Refereed)
    Abstract [en]

    The activity and adsorption of three variants of human carbonic anhydrase (HCA) with similar topology but variation in charge and stability were studied in the presence of carboxyl-modified polystyrene nanoparticles of different sizes ranging from 25 nm to 114 nm. The balance of forces driving the adsorption of carbonic anhydrase variants is affected by the physicochemical properties of the protein and the nanoparticle size. All enzymes are totally inhibited upon adsorption due to the transition towards a molten globule like state that lacks enzymatic activity. The size of the particle affects the adsorption of human carbonic anhydrase I and N-terminal truncated human carbonic anhydrase II. Investigations on pH effects indicate that the size of the particle modulates the lateral interactions at the protein layer for these particular variants whose adsorption is mainly driven by electrostatic forces. A third variant, human carbonic anhydrase II, instead shows no strong influence of nanoparticle size which supports an adsorption process mainly driven by the hydrophobic effect.

  • 56.
    Assarsson, Anna
    et al.
    Lund University.
    Hellstrand, Erik
    Lund University.
    Cabaleiro-Lago, Celia
    Lund University.
    Linse, Sara
    Lund University.
    Charge dependent retardation of amyloid β aggregation by hydrophilic proteins2014In: ACS Chemical Neuroscience, ISSN 1948-7193, E-ISSN 1948-7193, Vol. 5, no 4, p. 266-74Article in journal (Refereed)
    Abstract [en]

    The aggregation of amyloid β peptides (Aβ) into amyloid fibrils is implicated in the pathology of Alzheimer's disease. In light of the increasing number of proteins reported to retard Aβ fibril formation, we investigated the influence of small hydrophilic model proteins of different charge on Aβ aggregation kinetics and their interaction with Aβ. We followed the amyloid fibril formation of Aβ40 and Aβ42 using thioflavin T fluorescence in the presence of six charge variants of calbindin D9k and single-chain monellin. The formation of fibrils was verified with transmission electron microscopy. We observe retardation of the aggregation process from proteins with net charge +8, +2, -2, and -4, whereas no effect is observed for proteins with net charge of -6 and -8. The single-chain monellin mutant with the highest net charge, scMN+8, has the largest retarding effect on the amyloid fibril formation process, which is noticeably delayed at as low as a 0.01:1 scMN+8 to Aβ40 molar ratio. scMN+8 is also the mutant with the fastest association to Aβ40 as detected by surface plasmon resonance, although all retarding variants of calbindin D9k and single-chain monellin bind to Aβ40.

  • 57.
    Assarsson, Anna
    et al.
    Lund University.
    Linse, Sara
    Lund University.
    Cabaleiro-Lago, Celia
    Lund University.
    Effects of polyamino acids and polyelectrolytes on amyloid β fibril formation2014In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 30, no 29, p. 8812-8Article in journal (Refereed)
    Abstract [en]

    The fibril formation of the neurodegenerative peptide amyloid β (Aβ42) is sensitive to solution conditions, and several proteins and peptides have been found to retard the process. Aβ42 fibril formation was followed with ThT fluorescence in the presence of polyamino acids (poly-glutamic acid, poly-lysine, and poly-threonine) and other polymers (poly(acrylic acid), poly(ethylenimine), and poly(diallyldimethylammonium chloride). An accelerating effect on the Aβ42 aggregation process is observed from all positively charged polymers, while no effect is seen from the negative or neutral polymers. The accelerating effect is dependent on the concentration of positive polymer in a highly reproducible manner. Acceleration is observed from a 1:500 polymer to Aβ42 weight ratio and up. Polyamino acids and the other polymers exert quantitatively the same effect at the same concentrations based on weight. Fibrils are formed in all cases as verified by transmission electron microscopy. The concentrations of polymers required for acceleration are too low to affect the Aβ42 aggregation process through increased ionic strength or molecular crowding effects. Instead, the acceleration seems to arise from the locally increased Aβ42 concentration near the polymers, which favors association and affects the electrostatic environment of the peptide.

  • 58.
    Assarsson, Anna
    et al.
    Lund University.
    Pastoriza-Santos, Isabel
    Spain.
    Cabaleiro-Lago, Celia
    Lund University.
    Inactivation and adsorption of human carbonic anhydrase II by nanoparticles2014In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 30, no 31, p. 9448-56Article in journal (Refereed)
    Abstract [en]

    The enzymatic activity of human carbonic anhydrase II (HCAII) was studied in the presence of nanoparticles of different nature and charge. Negatively charged nanoparticles inhibit HCAII whereas no effect is seen for positively charged particles. The kinetic effects were correlated with the strength of binding of the enzyme to the particle surface as measured by ITC and adsorption assays. Moreover, conformational changes upon adsorption were observed by circular dichroism. The main initial driving force for the adsorption of HCAII to nanoparticles is of electrostatic nature whereas the hydrophobic effect is not strong enough to drive the initial binding. This is corroborated by the fact that HCAII do not adsorb on positively charged hydrophobic polystyrene nanoparticles. Furthermore, the dehydration of the particle and protein surface seems to play an important role in the inactivation of HCAII by carboxyl-modified polystyrene nanoparticles. On the other hand, the inactivation by unmodified polystyrene nanoparticles is mainly driven by intramolecular interactions established between the protein and the nanoparticle surface upon conformational changes in the protein.

  • 59.
    Atxutegi, Eneko
    et al.
    Spanien.
    Grinnemo, Karl-Johan
    Karlstads universitet.
    Izurza, Andoni
    Spanien.
    Arvidsson, Åke
    Kristianstad University, School of Health and Society, Avdelningen för Design och datavetenskap.
    On the move with TCP in current and future mobile networks2017In: Proceedings of the 8th International Conference on the Network of the Future, London, United Kingdom, November 2017, 2017Conference paper (Other academic)
    Abstract [en]

    Mobile wireless networks constitute an indispensable part of the global Internet, and with TCP the dominating transport protocol on the Internet, it is vital that TCP works equally well over these networks as over wired ones. This paper identifies the performance dependencies by analyzing the responsiveness of TCP NewReno and TCP CUBIC when subject to bandwidth variations related to movements in different directions. The presented evaluation complements previous studies on 4G mobile networks in two important ways: It primarily focuses on the behavior of the TCP congestion control in medium- to high-velocity mobility scenarios, and it not only considers the current 4G mobile networks, but also low latency configurations that move towards the overall potential delays in 5G networks. The paper suggests that while both CUBIC and NewReno give similar goodput in scenarios where the radio channel continuously degrades, CUBIC gives a significantly better goodput in scenarios where the radio channel quality continuously increases. This is due to CUBIC probing more aggressively for additional bandwidth. Important for the design of 5G networks, the obtained results also demonstrate that very low latencies are capable of equalizing the goodput performance of different congestion control algorithms. Only in low latency scenarios that combine both large fluctuations of available bandwidths and a mobility pattern in which the radio channel quality continuously increases can some performance differences be noticed.

  • 60.
    Augustsson, Johanna
    Kristianstad University, Faculty of Natural Science.
    De öppna fältens rationalisering i områden med olika ägarstruktur i nordöstra Helsingborg under perioden 1947–2017: Med fokus på trädrader och habitatöar2019Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [sv]

    De senaste 50 åren har intensifieringen av jordbruket ökat i takt med maskinernas framfart. Detta på bekostnad av att livsviktiga biotoper för djur och växter försvunnit, då åkerlandskapet blivit allt mer homogent. Skogen och jordbrukslandskapet är två av de artrikaste landskapstyperna och utgör idag störst hot för Sveriges rödlistade arter. Undersökningsområdet ligger i nordvästra Skånes åkerlandskap och uppsatsen behandlar jordbruksmarkens öppna fält och dess utveckling. Uppsatsen belyser även skillnader mellan små- och medelstora jordbruk jämfört med storjordbruk i ett område bestående av Fleninge, Kropp, Holk och Rosendal, belägna i nordöstra Helsingborgs kommun. Undersökningsområdet är intressant för att studera olika markägares förhållningssätt till jordbruksmarken. En GIS-analys över områdets landskapsutveckling av öppna fält, trädrader och habitatöar presenteras för åren 1947, 1965 och 2017. Analysen visar att medelstorleken på de öppna fälten, betesmarken och åkermarken i båda undersökningsområdena har mer än fördubblats på 70 år, med störst förändring på storjordbruket. Det syns även tydligt att antalet fält successivt minskat med åren då 197 fält har försvunnit på de små- och medelstora jordbruken samtidigt som 69 försvunnit i storjordbruksområdet. En genomsnittlig minskning av trädrader, med cirka 129 meter per år, sker på det små- och medelstora jordbruket, där minskningen är störst. Habitatöarna går från att ha varit vanligare inom storjordbruket till att merparten försvinner under undersökningsperiodens senare del. På det små- och medelstora jordbruket ökar antalet habitatöar även om en stor andel habitatöar avlägsnas, så tillkommer många. År 2017 finns totalt 99 habitatöar i undersökningsområdet, vilket innebär en minskning med cirka 0,4 habitatöar per år. Habitatöarna har dock varit svåra att analysera, detta ser dock ut att hänga samman med att glesa typer av trädrader tillkommer där, vilka enligt gängse kategoriseringskriterier inte räknas till trädrader utan blir individuella habitatöar. Denna utveckling har troligen en negativ inverkan på den biologiska mångfalden i området. Då minskning av habitat och biotoper är ett av de största hoten för biodiversiteten och arters utrotning är det grundläggande att jordbruket istället bidrar till bevarande av arter för en hållbar framtid.

  • 61.
    Axelsson, Carolina
    et al.
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Research environment Man & Biosphere Health (MABH).
    Rehnstam-Holm, Ann-Sofi
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Research environment Man & Biosphere Health (MABH).
    Nilsson, B
    Lund University.
    Optimization of several parameters in order to reduce time in antibiotic susceptibility testing in a clinical laboratory2017Conference paper (Other academic)
    Abstract [en]

    Background - When sepsis or bacteraemia is suspected, patient blood samples are cultivated in blood culture bottles and then further incubated for identification of the organism and antimicrobial susceptibility testing. These methods are slow, identifying causative pathogens in a couple of hours, and antibiotic susceptibility results within 18-36 hours.

    Here we present optimization of several parameters in order to evaluate if the MBT ASTRA™ method can be a rapid tool, used for routine antibiotics susceptibility testing, in a clinical laboratory.

    Methods – MALDI-TOF MS measurements were performed with a Microflex LT/SH bench-top mass spectrometer (Bruker) with standard settings. The resulting spectra were uploaded in the MBT-ASTRA™ software, which normalizes the peaks and determines the AUC and RG values for each setup.

    Results - The bacterial preparation steps generated a new protocol, which reduced time with 30-60 minutes.

    The antibiotics susceptibility test was optimized for 90 minutes incubation time. 200 µl McFarland 0.5 bacterial suspension in broth were incubated in broth at 37°C, with and without 32 µg/ml Cefotaxime, 16 µg/ml Meropenem and 4 µg/ml Ciprofloxacin.

    The suspensions were transferred to 0.45 µm pore size filter membraned 96 well plate. They were centrifuged; washed; fixated and eluted; put on a MALDI-target, and covered by matrix solution. All could be automated with robot, which reduced time with 60 minutes.

    Conclusion – Rapid susceptibility testing becomes more requested with the increase of resistance bacteria causing infections. Our study can be a valuable tool for clinical laboratories striving for reduction in time handling of antibiotic susceptibility testing.

  • 62. Back, C
    et al.
    Boisvert, J
    Lacoursière, Jean O.
    Charpentier, G
    High-dosage treatment of a Quebec stream with Bacillus thuringiensis serovar. israelensis: efficacy against black fly larvae (Diptera, Simuliidae) and impact on nontarget insects1985In: Canadian Entomologist, ISSN 0008-347X, E-ISSN 1918-3240, Vol. 117, no 12, p. 1523-1534Article in journal (Refereed)
    Abstract [en]

    A typical lake outlet of the Canadian Shield was treated for 15 min with a high dose (5.28 g/L s−1 of discharge) of Teknar®, a commercial formulation of Bacillus thuringiensis serovar. israelensis. Efficacy on Simuliidae larvae and impact on non-target aquatic insects of this stream were monitored using drift nets, counting plates, and artificial turf substrates along a 1000-m section downstream of the site of application. Compared with a 4-day pre-treatment average for 12-h sampling periods, drift of Simuliidae increased from 64 to 92 ×, with shorter peaks of 133–184 ×, 2–6 h after treatment. There was no evident drift increase in larvae of Ephemeroptera, Plecoptera, Trichoptera, Chironomidae, or dipterous pupae, but larvae of Blephariceridae (Diptera) were severely affected as their drift was increased by up to 50 × and remained high for 3 days. After 30 h the mortality of Simuliidae on counting plates ranged from 95 to 82% in the first 300 m, with detachment rates of 78.5–46.5%. Densities of non-target insect larvae were not reduced on the artificial substrates, except for 2 genera of Chironomidae (Eukiefferella and Polypedilum) which were reduced 26 to 39% of their original density. Drifting larvae of 1 chironomid genus (Phaenopsectra) also showed symptoms of toxemia by B.t.i. The main impact of the treatment was thus seen in 2 Nematocera families (Chironomidae and Blephariceridae) which were mainly exposed to B.t.i. sedimented on the bottom of the stream or attached to periphyton growing on rocks.

  • 63.
    Bagewitz, Cornelia
    et al.
    Kristianstad University, Faculty of Natural Science.
    Gustavsson, Emelie
    Kristianstad University, Faculty of Natural Science.
    Metoder för minskat matsvinn: ett fokuserat fältarbete på gymnasieskolor2018Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    Introduction

    About one-third of all food produced for human consumption in the world is either wasted or lost. This means that large amounts of resources, which are used in the production of food, are used in vain. If the food waste would decrease it would reduce the carbon dioxide emissions, lower the energy- and raw material consumption and reduce the environmental impact. Food waste is not a new area of research, however, there is a need for more studies that address methods for reducing it. A big part of the food waste in Sweden comes from large-scale catering establishments, especially from schools, which leads to the aim of this study.

     

    Aim

    The aim is to study which methods a couple of upper secondary schools are using for reducing their food waste, the employees experience of the methods and their thoughts on measures of improvement.  

     

    Method

    Interviews and observations were carried out using focused ethnography in four upper secondary schools in Helsingborg. The collected data was reviewed using thematic analysis. The literature in the study was used as an analytical tool.

     

    Results

    The methods used by the schools consisted of different ways of serving, re-using food, smaller garbage cans, communication, competition, and measuring and keeping track of the food waste. 

     

    Conclusion

    The methods perceived as most efficient were smaller sized serving tools, utilizing and re-using food, smaller garbage cans and measuring the food waste, while spoons used to taste the food before serving yourself was not considered as useful. The communication between the school and the kitchen staff needs to improve to facilitate the task of reducing food waste. Lectures about food waste could be a way to involve the students in the task.

  • 64.
    Bak, Søren Alex
    et al.
    Analytical Bioscience, Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen.
    Björklund, Erland
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. Kristianstad University, Faculty of Natural Science, Research environment MoLab.
    Occurrence of ionophores in the Danish environment2014In: Antibiotics, ISSN 2079-6382, Vol. 3, no 4, p. 564-571Article in journal (Refereed)
    Abstract [en]

    Antibiotics in the environment are a potential threat to environmental ecosystems as well as human health and safety. Antibiotics are designed to have a biological effect at low doses, and the low levels detected in the environment have turned focus on the need for more research on environmental occurrence and fate, to assess the risk and requirement for future regulation. This article describes the first occurrence study of the antibiotic polyether ionophores (lasalocid, monensin, narasin, and salinomycin) in the Danish environment. Various environmental matrices (river water, sediment, and soil) have been evaluated during two different sampling campaigns carried out in July 2011 and October 2012 in an agricultural area of Zealand, Denmark. Lasalocid was not detected in any of the samples. Monensin was measured at a concentration up to 20 ng·L−1 in river water and 13 µg·kg−1 dry weight in the sediment as well as being the most frequently detected ionophore in the soil samples with concentrations up to 8 µg·kg−1 dry weight. Narasin was measured in sediment samples at 2 µg·kg−1 dry weight and in soil between 1 and 18 µg·kg−1 dry weight. Salinomycin was detected in a single soil sample at a concentration of 30 µg·kg−1 dry weight.

  • 65.
    Bao, Leiming
    et al.
    Kristianstad University, School of Health and Society.
    Sun, Chunyan
    Kristianstad University, School of Health and Society.
    Human-Computer Interaction in a Smart House2012Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    The quality attribute concerning usability is generally of significant importance to systems. The area of Human Computer Interaction, HCI, especially handles several usability aspects. This degree project emphasizes HCI in a context of, so called, Smart House. The report is divided into three main sections: theory, application, and measurement results. In the theory section we will present about the context of HCI and the content of HCI, such as HCI model and goals of HCI design. In the application section we discuss the use of mobile phones as a device to remotely control devices of smart houses, and present a system developed to support such services. In order to make the system more attractive, we decided to design it for two categories of operations, menu operation and direct-touch operation mode. Finally, we have used questionnaires for reasons of measuring user satisfaction. Through investigation and analysis of the result of this, we come to the conclusion that system usability is good.

  • 66.
    Barclay, C J
    et al.
    Griffith University, Gold Coast, QLD, Australia.
    Widén, Cecilia
    Sveriges lantbruksuniversitet, Kristianstad.
    Efficiency of cross-bridges and mitochondria in mouse cardiac muscle2010In: Muscle biophysics: from molecules to cells / [ed] D.E. Rassier, Springer Berlin/Heidelberg, 2010, Vol. 682, p. 267-278Chapter in book (Refereed)
    Abstract [en]

    The aim of this study was to make cellular-level measurements of the mechanical efficiency of mouse cardiac muscle and to use these measurements to determine (1) the work performed by a cross-bridge in one ATP-splitting cycle and (2) the fraction of the free energy available in metabolic substrates that is transferred by oxidative phosphorylation to free energy in ATP (i.e. mitochondrial thermodynamic efficiency). Experiments were performed using isolated left ventricular mouse papillary muscles (n = 9; studied at 27°C) and the myothermic technique. The production of work and heat was measured during and after 40 contractions at a contraction frequency of 2 Hz. Each contraction consisted of a brief isometric period followed by isovelocity shortening. Work output, heat output and enthalpy output were all independent of shortening velocity. Maximum initial mechanical efficiency (mean ± SEM) was 31.1 ± 1.3% and maximum net mechanical efficiency 16.9 ± 1.5%. It was calculated that the maximum work per cross-bridge cycle was 20 zJ, comparable to values for mouse skeletal muscle, and that mitochondrial thermodynamic efficiency was 72%. Analysis of data in the literature suggests that mitochondrial efficiency of cardiac muscle from other species is also likely to be between 70 and 80%.

  • 67.
    Barrhök, Johan
    Kristianstad University College, Department of Mathematics and Science.
    Från ett nödvändigt ont, till en naturtyp värd att bevara - en studie av ljungheden i Sandsjöbacka och Grönabur/From a necessary evil to a nature type worth protect - a studie of the Calluna heath of Sandsjöbacka & Grönabur2005Independent thesis Basic level (degree of Bachelor)Student thesis
    Abstract [sv]

    In the middle of the 19th century was Calluna heath the major nature type in Halland, but the remaining parts today is only 1 500 hectares which is a 99 % decrease of the Calluna heath mainly due to an increase in forest plantations and arable fields. I have made a quantitative study how the Calluna heath of Grönabur & Sandsjöbacka has changed between 1841 and 2005 and why it didn’t disappear in the same manner, as it did in other parts of Halland.

    The studying of original sources as historical maps, literature and by doing field visits has been significant for the final result. The main reason for the different development of the Calluna heath in the investigation area (69 % decrease), compare to elsewhere in Halland, is the establishment and management of a nature reserve.

  • 68.
    Bechshoft, Thea
    et al.
    Danmark.
    Wright, Andrew J.
    Danmark.
    Weisser, Johan J.
    Danmark.
    Teilmann, Jonas
    Danmark.
    Dietz, Rune
    Danmark.
    Hansen, Martin
    Danmark.
    Björklund, Erland
    Kristianstad University, Plattformen för molekylär analys. Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap. Kristianstad University, Faculty of Natural Science, Research environment MoLab. Danmark.
    Styrishave, Bjarne
    Danmark.
    Developing a new research tool for use in free-ranging cetaceans: recovering cortisol from harbour porpoise skin2015In: Conservation Physiology, E-ISSN 2051-1434, Vol. 3Article in journal (Refereed)
    Abstract [en]

    We developed a chemical analytical procedure for sampling, extracting and determining epidermal skin cortisol concentrations (SCCs) in the harbour porpoise (Phocoena phocoena) using gas chromatography–tandem mass spectrometry. In brief, this involved a pressurized liquid extraction with a two-step solid-phase clean-up. A derivatization step was conducted prior to detection. To evaluate the new assay, cortisol was analysed in three different sample types obtained from four harbour porpoises: skin plates, dorsal fin skin plugs (with and without lidocaine) and epidermal scrapes. Skin cortisol concentrations could be measured using the new assay in the majority of the tested skin samples down to a minimal sample size of 49 mg dry weight (dw). Water content ranged from 10 to 46% in the plug samples, which had SCCs from 2.1 to 77.7 ng/g dw. Epidermal scrape samples had the highest water content (83–87%) and lower SCCs (0.6–15 ng/g dw), while the skin plates had intermediate water contents (60–66%) and SCCs of 2.6–13.0 ng/g dw. SCC was slightly higher in plugs with lidocaine than without (average values of 41 and 33 ng/g dw, respectively). Substantial within-individual variations in cortisol concentrations are also common in other matrices such as blood and hair. Some important factors behind this variation could be e.g. the animal's sex, age, body condition, reproductive stage, and the body region sampled, as well as season, moulting cycles and water temperature. Clearly, more research into SCCs is required. The findings described here represent the first critical steps towards using epidermal skin cell samples to assess chronic stress levels in cetaceans and the development of a widely applicable health-assessment tool in these species.

  • 69.
    Beery, Thomas
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Research environment Learning in Science and Mathematics (LISMA). Kristianstad University, Research environment Man & Biosphere Health (MABH).
    Childhood collecting in nature: quality experience in important places2016Conference paper (Refereed)
    Abstract [en]

    There is growing concern for both the decline of direct experience people have with nature, and the quality of that experience. This diminished experience has negative consequences for public awareness and concern for biodiversity and ecosystem health (Miller, 2005). At the same time, a diminished experience of nature appears to have a substantial negative impact on child development (Kahn & Friedman, 1995; Kahn, 2002; Matteo, Barthel, & Lars, 2014; Pyle, 1993; Thomashow, 2002). These concerns are heightened in the urban context where increased urbanization shows a relationship with a reduction in biodiversity and ecosystem health (MA, 2005; Sala et al., 2000). Additional concern comes from studies showing decreasing ecological knowledge among growing urban populations (McDaniel & Alley, 2005; McKinney, 2002). In an attempt to address these concerns and contribute toward a better understanding of the importance of childhood experience of nature, this study investigates one specific example, collecting in nature. Studies show that childhood collecting in nature (the gathering of rocks, shells, feathers, etc. as part of play and free exploration) is a widespread phenomenon (Lekies & Beery, 2013), and yet, very little is understood about this behavior. This study explored the details of childhood collecting in nature with an emphasis on the places of this experience. Participants consisted of a random sample of undergraduate students at a Swedish university (N = 380) participating in a survey focused upon early life outdoor experiences. Responses included multiple choice and Likert scale items, along with data from open-ended questions. In addition, participants were invited to discuss their experience of childhood collecting in greater detail via a semi-structured interview. Fourteen interviews were conducted as follow-up to the survey. Data review considered descriptive statistics, correlations, and regression analysis triangulated with the qualitative data from the open-ended responses and interviews. Results highlight the importance of specific places in the childhood experience of nature, the importance of nearby nature, and further, provide preliminary support for a model for environmental concern (Wolf-Watz, 2015). Ultimately, the study illuminates the idea of childhood development as a cultural ecosystem service and provides implications for nature-based solutions, such as green infrastructure, to support childhood nature experience.

  • 70.
    Beery, Thomas
    Kristianstad University.
    Exploring the potential of nature-based outdoor recreation to serve the Kristianstad Vattenrike goals: A report on analysis of 2013 Naturum visitor data.2014Report (Refereed)
  • 71.
    Beery, Thomas
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap.
    People in nature: relational discourse for outdoor educators2014In: Research in Outdoor Education, ISSN 2375-6381, Vol. 12, no 1, p. 1-14Article in journal (Other academic)
    Abstract [en]

    Outdoor educators are concerned about a perceived human disconnection from nature. There is awareness of a lack of human affiliation, connection, or identity with nonhuman nature and its impact on attitudes and behaviors. This essay raises the possibility that despite our concern, we may contribute toward this disconnection via language that supports a separation of the natural and the cultural. Our ability to separate ourselves conceptually from the rest of nature may be partially to blame for environmental degradation, therefore challenging the nature-culture dichotomy is both useful and constructive. This essay will present examples of how outdoor educators can attempt to get past this problematic dichotomy and motivate more relational discourse within the practice of outdoor education.

  • 72.
    Beery, Thomas
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Research environment Learning in Science and Mathematics (LISMA). Kristianstad University, Research environment Man & Biosphere Health (MABH).
    Urban Nature Needs: Does the Path Through a Nature Center Lead Out the Door?2015Conference paper (Refereed)
  • 73.
    Beery, Thomas H.
    et al.
    Kristianstad University, Faculty of Natural Science, Research environment Man & Biosphere Health (MABH). Kristianstad University, Faculty of Education, Avdelningen för matematik- och naturvetenskapernas didaktik. USA.
    Lekies, Kristi S.
    USA.
    Childhood collecting in nature: quality experience in important places2019In: Children's Geographies, ISSN 1473-3285, E-ISSN 1473-3277, Vol. 17, no 1, p. 118-131Article in journal (Refereed)
    Abstract [en]

    A need for a more specific understanding of childhood geographies has motivated an investigation of one activity, childhood collecting in nature. This study examined collecting behavior, places of collecting, and the relationship of these places to environmental connectedness in adulthood. Topophilia is presented as a guide to help consider why children collect in nature and to expand upon a limited understanding of collecting behavior. These ideas are explored with a mixed-method design strategy involving surveys and semi-structured interviews with a sample of Swedish university students. Results show collecting in nature to be a widespread, meaningful, and memorable experience in the formative years of participants. Results also demonstrate potential support for topophilia as a way to understand the childhood collecting nature phenomenon. Implications include recognition of the importance of family to support children’s engagement in the natural world and proximate access to nature as a critical aspect of childhood experience.

  • 74.
    Beery, Thomas
    et al.
    Kristianstad University, Research environment Man & Biosphere Health (MABH). Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap.
    Jönsson, Ingemar
    Kristianstad University, Research environment Man & Biosphere Health (MABH). Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap.
    Upplevelsen av biologisk mångfald2015In: Vattenriket i Fokus, ISSN 1653-9338, Vol. 2015, no 4, p. 39-43Article in journal (Other (popular science, discussion, etc.))
    Abstract [en]

    It is important to do something outside, not only inside this building...if you are outside the nature is talking to you. (Arabic/English speaking visitor)

    Concern for a diminished nature experience has been referred to as an extinction of experience (Pyle, 1993). The concern is based on the fear that diminished experience of nature leads to reduced environmental awareness and knowledge, and ultimately, to a reduction in pro-environmental behavior. Attention to both the value and potential loss of nature experience is at the foundation of the research presented here.

     

    Two related studies are briefly presented that explore the experience of nature, and specifically, the experience of biodiversity in the Kristianstad Vattenrike biosphere reserve. The first is a study of the relationship between place attachment and participation in nature based outdoor recreation. Random and targeted field based surveys with residents of the Kristianstad municipality were used to gather information. Results indicated a positive and significant relationship between measures of place attachment and nature-based outdoor recreation. The second study, an investigation of the Swedish EPA mandated goal that Swedish Nature Centers (Naturum) will inspire or motivate a direct experience of nature was conducted using thought listing methods. The results of these interviews indicated that the nature center in the Kristianstad Vattenrike is serving this noted function. An outcome that links both studies are the results that highlight the importance of proximate access (in regard to residence and transportation) of recreation and outdoor opportunity to facilitate direct experiences of nature.

  • 75.
    Beery, Thomas
    et al.
    Kristianstad University, Research environment Man & Biosphere Health (MABH). Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap.
    Jönsson, K. Ingemar
    Kristianstad University, Research environment Man & Biosphere Health (MABH). Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap.
    Betydelsen av att uppleva biologisk mångfald2015In: Biodiverse, ISSN 1401-5064, Vol. 20, no 2, p. 16-17Article in journal (Other (popular science, discussion, etc.))
  • 76.
    Beery, Thomas
    et al.
    Kristianstad University, Research environment Man & Biosphere Health (MABH). Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap.
    Jönsson, K. Ingemar
    Kristianstad University, Research environment Man & Biosphere Health (MABH). Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap.
    Inspiring the outdoor experience: does the path through a nature center lead out the door?2015In: Journal of Interpretation Research, ISSN 1092-5872, Vol. 20, no 1, p. 67-85Article in journal (Refereed)
    Abstract [en]

    This study investigates the visitor experience at a Swedish nature center within aUNESCO biosphere reserve. The question of whether this interpretive facility succeedsin motivating the visitor to get outdoors for a direct experience of nature is explored. Useof the environmental connectedness perspective and concerns about diminished natureexperience support the importance of this study. A number of qualitative methodologiesare used to investigate the research questions, including thought listing, phenomenology,and field observation. Results indicate that this particular nature center generallysucceeded in the goal of inspiring visitors for a direct nature experience. The success inmotivating visitors appears to be a result of a number of key variables, including placebasedexhibitry, access, and personal visitor factors. Given the setting for this study, weconclude that interpretive nature centers have the potential to play an important role inthe re-imagination of urban environments.

  • 77.
    Beery, Thomas
    et al.
    Kristianstad University, Research environment Man & Biosphere Health (MABH). Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap.
    Jönsson, K. Ingemar
    Kristianstad University, Research environment Man & Biosphere Health (MABH). Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap.
    Topophilia and human affiliation with nature2015Conference paper (Refereed)
    Abstract [en]

    Objective: The objective of this study is to explore the co-evolutionary foundation for place-based human affiliation with nonhuman nature, and its potential to support sustainable development at the local level. In particular, we analyse the Topophilia Hypothesis, an expansion of the Biophilia Hypothesis which includes also non-living elements in the environment.

    Methods: The study represents a multidisciplinary conceptual analysis of how biological selection and cultural learning may have interacted during human evolution to promote adaptive mechanisms for human affiliation with nonhuman nature via specific place attachment. 

    Results and Conclusions: The Biophilia Hypothesis has been one of the most important theories of human connectedness with nature, suggesting a genetically based inclination for human affiliation with the biological world. The Topophilia Hypothesis has extended the ideas of Biophilia to incorporate a broader conception of nonhuman nature and a co-evolutionary theory of genetic response and cultural learning. It also puts more emphasis on affiliation processes with the local environment. We propose that nurturing potential topophilic tendencies may be a useful method to promote sustainable development at the local level, and ultimately at the global level. Tendencies of local affiliation may also have implications for multifunctional landscape management, an important area within sustainability research, and we provide some examples of successful landscape management with a strong component of local engagement. Since human affiliation with nonhuman nature is considered an important dimension of environmental concern and support for pro-environmental attitudes, the Topophilia Hypothesis may provide a fruitful ground for a discourse within which scholars from many scientific fields, including human evolution and humanistic geography, can participate.

     

  • 78.
    Beery, Thomas
    et al.
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap.
    Jönsson, K. Ingemar
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap.
    Elmberg, Johan
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap.
    From environmental connectedness to sustainable futures: topophilia and human affiliation with nature2015In: European Human Behaviour and Evolution Association Annual Conference, University of Helsinki, March 29-April 1, 2015, 2015, p. 57-58Conference paper (Refereed)
    Abstract [en]

    The objective of this study is to explore the co-evolutionary foundation for place-based human affiliation with nonhuman nature, and its potential to support sustainable development at the local level. In particular, we analyse the Topophilia Hypothesis, an expansion of the Biophilia Hypothesis which includes also non-living elements in the environment. Methods: The study represents a multidisciplinary conceptual analysis of how biological selection and cultural learning may have interacted during human evolution to promote adaptive mechanisms for human affiliation with nonhuman nature via specific place attachment. Results and Conclusions: The Biophilia Hypothesis has been one of the most important theories of human connectedness with nature, suggesting a genetically based inclination for human affiliation with the biological world. The Topophilia Hypothesis has extended the ideas of Biophilia to incorporate a broader conception of nonhuman nature and a co-evolutionary theory of genetic response and cultural learning. It also puts more emphasis on affiliation processes with the local environment. We propose that nurturing potential topophilic tendencies may be a useful method to promote sustainable development at the local level, and ultimately at the global level. Tendencies of local affiliation may also have implications for multifunctional landscape management, an important area within sustainability research, and we provide some examples of successful landscape management with a strong component of local engagement. Since human affiliation with nonhuman nature is considered an important dimension of environmental concern and support for pro-environmental attitudes, the Topophilia Hypothesis may provide a fruitful ground for a discourse within which scholars from many scientific fields, including human evolution and humanistic geography, can participate.

  • 79.
    Beery, Thomas
    et al.
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Research environment Man & Biosphere Health (MABH).
    Jönsson, K. Ingemar
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Research environment Man & Biosphere Health (MABH).
    Elmberg, Johan
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Research environment Man & Biosphere Health (MABH).
    From environmental connectedness to sustainable futures: topophilia and human affiliation with nature2015In: Sustainability, ISSN 2071-1050, E-ISSN 2071-1050, Vol. 7, no 7, p. 8837-8854Article in journal (Refereed)
    Abstract [en]

    Human affiliation with nonhuman nature is an important dimension of environmental concern and support for pro-environmental attitudes. A significant theory of human connectedness with nature, the Biophilia Hypothesis, suggests that there exists a genetically based inclination for human affiliation with the biological world. Both support and challenge to the Biophilia Hypothesis are abundant in the literature of environmental psychology. One response that both challenges and builds upon the Biophilia Hypothesis is the Topophilia Hypothesis. The Topophilia Hypothesis has extended the ideas of biophilia to incorporate a broader conception of nonhuman nature and a co-evolutionary theory of genetic response and cultural learning. While the Topophilia Hypothesis is a new idea, it is built upon long-standing scholarship from humanistic geography and theories in human evolution. The Topophilia Hypothesis expands previous theory and provides a multidisciplinary consideration of how biological selection and cultural learning may have interacted during human evolution to promote adaptive mechanisms for human affiliation with nonhuman nature via specific place attachment. Support for this possible co-evolutionary foundation for place-based human affiliation with nonhuman nature is explored from multiple vantage points. We raise the question of whether this affiliation may have implications for multifunctional landscape management. Ultimately, we propose that nurturing potential topophilic tendencies may be a useful method to promote sustainable efforts at the local level with implications for the global.

  • 80.
    Beery, Thomas
    et al.
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Research environment Man & Biosphere Health (MABH).
    Raymond, Christopher M
    Swedish University of Agricultural Sciences.
    Kyttä, Marketta
    Finland.
    Olafsson, Anton Stahl
    Danmark.
    Plieninger, Tobias
    Danmark.
    Sandberg, Mattias
    Gothenburg University.
    Stenseke, Marie
    Gothenburg University.
    Tengö, Maria
    Stockholm Resilience Center.
    Jönsson, K. Ingemar
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Research environment Man & Biosphere Health (MABH).
    Fostering incidental experiences of nature through green infrastructure planning2017In: Ambio, ISSN 0044-7447, E-ISSN 1654-7209, Vol. 46, no 7, p. 717-730Article in journal (Refereed)
    Abstract [en]

    Concern for a diminished human experience of nature and subsequent decreased human well-being is addressed via a consideration of green infrastructure's potential to facilitate unplanned or incidental nature experience. Incidental nature experience is conceptualized and illustrated in order to consider this seldom addressed aspect of human interaction with nature in green infrastructure planning. Special attention has been paid to the ability of incidental nature experience to redirect attention from a primary activity toward an unplanned focus (in this case, nature phenomena). The value of such experience for human well-being is considered. The role of green infrastructure to provide the opportunity for incidental nature experience may serve as a nudge or guide toward meaningful interaction. These ideas are explored using examples of green infrastructure design in two Nordic municipalities: Kristianstad, Sweden, and Copenhagen, Denmark. The outcome of the case study analysis coupled with the review of literature is a set of sample recommendations for how green infrastructure can be designed to support a range of incidental nature experiences with the potential to support human well-being.

  • 81.
    Beery, Thomas
    et al.
    Kristianstad University, Research environment Man & Biosphere Health (MABH). Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Research environment Learning in Science and Mathematics (LISMA).
    Stålhammar, Sanna
    Lund University.
    Jönsson, Ingemar
    Kristianstad University, Research environment Man & Biosphere Health (MABH). Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap.
    Wamsler, Christine
    Lund University.
    Bramryd, Torleif
    Lund University.
    Brink, Ebba
    Lund University.
    Ekelund, Nils
    Malmö University.
    Johansson, Michael
    Lund University.
    Palo, Thomas
    SLU.
    Schubert, Per
    Malmö University.
    Perceptions of the ecosystem services concept: opportunities and challenges in the Swedish municipal context2016In: Ecosystem Services, ISSN 2212-0416, E-ISSN 2212-0416, Vol. 17, p. 123-130Article in journal (Refereed)
    Abstract [en]

    A current focus of ecosystem services (ES) implementation is on the municipal level of government where international and national legislation and policies have to be translated into practice. Given this focus, an understanding of perceptions within municipalities of the ES concept is crucial to support the implementation process. Against this background, this paper examines the perceptions of Swedish municipal stakeholders for the ES concept. A 2013 Swedish federal mandate that states that the values of ecosystem services should be considered in relevant decision-making processes, provides a timely context. Current perceptions, preconditions and awareness are explored via interviews and analyses. The results show that the views on the ecosystem services concept and its usefulness are generally very positive. Conceptual knowledge use is perceived as important as is the recognition of monetary valuation of ES. However, clarification of the distinction between implicit and explicit use of the concept by stakeholders is needed. Finally, results indicate that a deeper understanding of monetary valuation of ecosystem services by municipal staff members is connected with a more critical view on monetary valuation. It is concluded that detailed and clear definitions and guidelines are needed in order to support the process of implementing ES in municipalities.

  • 82.
    Beery, Thomas
    et al.
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Research environment Man & Biosphere Health (MABH).
    Wolf-Watz, Daniel
    Mid Sweden University.
    Nature to place: rethinking the environmental connectedness perspective2014In: Journal of Environmental Psychology, ISSN 0272-4944, E-ISSN 1522-9610, Vol. 40, no December, p. 198-205Article in journal (Refereed)
    Abstract [en]

    The environmental connectedness perspective posits that direct encounter with generalized, or non-specific “nature,” leads to environmental connectedness and subsequent pro-environmental behavior. This article examines this perspective and proposes a place-based application of the nature encounter-environmental behavior relation. An empirical study using data from a national survey on outdoor recreation and nature-based tourism is presented. Results show a minimal relationship between measures of environmental connectedness and self-reports of environmental behavior. The following examination of the environmental connectedness perspective reveals that environmental connectedness is rooted in a material/objective perspective, neglecting the human domain of perceptions, values, and representations. The environment as “nature” is portrayed as a geographically undefined agent with the inherent power to change human attitudes and behavior. Based on this, the article concludes with a proposed replacement of the elusive concept of nature for the relational concept of place.

  • 83.
    Beltran-Pardo, Eliana
    et al.
    Pontificia Universidad Javeriana, Instituto de Genética Humana, Bogotá.
    Jönsson, Ingemar
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Forskningsmiljön Man and Biosphere Health (MABH).
    Wojcik, Andrzej
    Department of Genetics, Microbiology and Toxicology, Stockholm University.
    Haghdoost, Siamak
    Department of Genetics, Microbiology and Toxicology, Stockholm University.
    Bermúdez-Cruz, Rosa María
    4Departamento de Genéticas y Biología Molecular, Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional - CINVESTAV, Mexico City.
    Bernal Villegas, Jaime E.
    Pontificia Universidad Javeriana, Instituto de Genética Humana, Bogotá.
    Sequence analysis of the DNA-repair gene rad51 in the tardigrades Milnesium cf. tardigradum, Hypsibius dujardini and Macrobiotus cf. harmsworthi2013In: Journal of limnology, ISSN 1129-5767, E-ISSN 1723-8633, Vol. 72, no s1, p. 80-91Article in journal (Refereed)
    Abstract [en]

    Tardigrades are known for being resistant to extreme conditions, including tolerance to ionising and UV radiation in both the hydratedand the dehydrated state. It is known that these factors may cause damage to DNA. It has recently been shown that single and double DNAstrand breaks occur when tardigrades are maintained for a long time in the anhydrobiotic state. This may suggest that perhaps tardigrades rely on efficient DNA repair mechanisms. Among all proteins that comprise the DNA repair system, recombinases such as RecA or Rad51 have a very important function: DNA exchange activity. This enzyme is used in the homologous recombination and allows repair of thedamaged strand using homologous non-damaged strands as a template. In this study, Rad51 induction was evaluated by western blot in Milnesium cf. tardigradum, after exposure to gamma radiation. The Rad51 protein was highly induced by radiation, when compared to the control. The rad51 genes were searched in three tardigrades: Milnesium cf. tardigradum, Hypsibius dujardini and Macrobiotus cf. harmsworthi. The gene sequences were obtained by preparing and sequencing transcriptome libraries for H. dujardini and M. cf. harmsworthi and designing rad51 degenerate primers specific for M. cf. tardigradum. Comparison of Rad51 putative proteins from tardigrades with other organisms showed that they are highly similar to the corresponding sequence from the nematode Trichinella spiralis. A structure-based sequence alignment from tardigrades and other organisms revealed that putative Rad51 predicted proteins from tardigrades contain the expected motifs for these important recombinases. In a cladogram tree based on this alignment, tardigrades tend to cluster together suggesting that they have selective differences in these genes that make them diverge between species. Predicted Rad51 structures from tardigrades were also compared with crystalline structure of Rad51 in Saccharomyces cerevisiae. These results reveal that S. cerevisiae Rad51 structure is very similar to that of the three analysed tardigrades. On the other hand the predicted structure of Rad51 from M. cf. harmsworthi and H. dujardini are closer related to each other, than each of them to that of M. cf. tardigradum.

  • 84.
    Beltrán-Pardo, Eliana
    et al.
    Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University.
    Jönsson, K. Ingemar
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University.
    Harms-Ringdahl, Mats
    Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University.
    Haghdoost, Siamak
    Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University.
    Wojcik, Andrzej
    Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University.
    Tolerance to gamma radiation in the tardigrade Hypsibius dujardini from embryo to adult correlate inversely with cellular proliferation2015In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 10, no 7, p. e0133658-Article in journal (Refereed)
    Abstract [en]

    Tardigrades are highly tolerant to desiccation and ionizing radiation but the mechanisms of this tolerance are not well understood. In this paper, we report studies on dose responses of adults and eggs of the tardigrade Hypsibius dujardini exposed to gamma radiation. In adults the LD50/48h for survival was estimated at ~ 4200 Gy, and doses higher than 100 Gy reduced both fertility and hatchability of laid eggs drastically. We also evaluated the effect of radiation (doses 50 Gy, 200 Gy, 500 Gy) on eggs in the early and late embryonic stage of development, and observed a reduced hatchability in the early stage, while no effect was found in the late stage of development. Survival of juveniles from irradiated eggs was highly affected by a 500 Gy dose, both in the early and the late stage. Juveniles hatched from eggs irradiated at 50 Gy and 200 Gy developed into adults and produced offspring, but their fertility was reduced compared to the controls. Finally we measured the effect of low temperature during irradiation at 4000 Gy and 4500 Gy on survival in adult tardigrades, and observed a slight delay in the expressed mortality when tardigrades were irradiated on ice. Since H.dujardini is a freshwater tardigrade with lower tolerance to desiccation compared to limno-terrestrial tardigrades, the high radiation tolerance in adults, similar to limno-terrestrial tardigrades, is unexpected and seems to challenge the idea that desiccation and radiation tolerance rely on the same molecular mechanisms. We suggest that the higher radiation tolerance in adults and late stage embryos of H.dujardini (and in other studied tardigrades) compared to early stage embryos may partly be due to limited mitotic activity, since tardigrades have a low degree of somatic cell division (eutely), and dividing cells are known tobe more sensitive to radiation.

  • 85.
    Beltrán-Pardo, Eliana
    et al.
    Instituto de Genética Humana, Pontificia Universidad Javeriana, Bogotá.
    Jönsson, K. Ingemar
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Forskningsmiljön Man and Biosphere Health (MABH).
    Wojcik, Andrzej
    Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University.
    Haghdoost, Siamak
    Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University.
    Harms-Ringdahl, Mats
    Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University.
    Bermúdez-Cruz, Rosa M.
    Departamento de Genética y Biología Molecular, Centro de Investigación y Estudios Avanzados, CINVESTAV, México D.F..
    Bernal Villegas, Jaime E.
    Instituto de Genética Humana, Pontificia Universidad Javeriana, Bogotá.
    Effects of ionizing radiation on embryos of the tardigrade Milnesium cf. tardigradum at different stages of development2013In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 8, no 9, p. e72098-Article in journal (Refereed)
    Abstract [en]

    Tardigrades represent one of the most desiccation and radiation tolerant animals on Earth, and several studies havedocumented their tolerance in the adult stage. Studies on tolerance during embryological stages are rare, but differentialeffects of desiccation and freezing on different developmental stages have been reported, as well as dose-dependent effectof gamma irradiation on tardigrade embryos. Here, we report a study evaluating the tolerance of eggs from theeutardigrade Milnesium cf. tardigradum to three doses of gamma radiation (50, 200 and 500 Gy) at the early, middle, andlate stage of development. We found that embryos of the middle and late developmental stages were tolerant to all doses,while eggs in the early developmental stage were tolerant only to a dose of 50 Gy, and showed a declining survival withhigher dose. We also observed a delay in development of irradiated eggs, suggesting that periods of DNA repair might havetaken place after irradiation induced damage. The delay was independent of dose for eggs irradiated in the middle and latestage, possibly indicating a fixed developmental schedule for repair after induced damage. These results show that thetolerance to radiation in tardigrade eggs changes in the course of their development. The mechanisms behind this patternare unknown, but may relate to changes in mitotic activities over the embryogenesis and/or to activation of responsemechanisms to damaged DNA in the course of development.

  • 86.
    Bengtsson, Daniel
    et al.
    Linnaeus University.
    Avril, Alexis
    Linnaeus University.
    Gunnarsson, Gunnar
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Research environment Man and Biosphere Health (MABH).
    Elmberg, Johan
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Research environment Man and Biosphere Health (MABH).
    Söderquist, Pär
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Research environment Man and Biosphere Health (MABH).
    Norevik, Gabriel
    Ottenby Bird Observatory.
    Tolf, Conny
    Linnaeus University.
    Safi, Kamran
    Max Planck Institute for Ornithology.
    Fiedler, Wolfgang
    Max Planck Institute for Ornithology.
    Wikelski, Martin
    Max Planck Institute for Ornithology.
    Olsen, Bjorn
    Uppsala University.
    Waldenstrom, Jonas
    Linnaeus University.
    Movements, home-range size and habitat selection of mallards during autumn migration2014In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 9, no 6, p. e100764-Article in journal (Refereed)
    Abstract [en]

    The mallard (Anas platyrhynchos) is a focal species in game management, epidemiology and ornithology, but comparably little research has focused on the ecology of the migration seasons. We studied habitat use, time-budgets, home-range sizes, habitat selection, and movements based on spatial data collected with GPS devices attached to wild mallards trapped at an autumn stopover site in the Northwest European flyway. Sixteen individuals (13 males, 3 females) were followed for 15-38 days in October to December 2010. Forty-nine percent (SD = 8.4%) of the ducks' total time, and 85% of the day-time (SD = 28.3%), was spent at sheltered reefs and bays on the coast. Two ducks used ponds, rather than coast, as day-roosts instead. Mallards spent most of the night (76% of total time, SD = 15.8%) on wetlands, mainly on alvar steppe, or in various flooded areas (e.g. coastal meadows). Crop fields with maize were also selectively utilized. Movements between roosting and foraging areas mainly took place at dawn and dusk, and the home-ranges observed in our study are among the largest ever documented for mallards (mean = 6,859 ha; SD = 5,872 ha). This study provides insights into relatively unknown aspects of mallard ecology. The fact that autumn-staging migratory mallards have a well-developed diel activity pattern tightly linked to the use of specific habitats has implications for wetland management, hunting and conservation, as well as for the epidemiology of diseases shared between wildlife and domestic animals.

  • 87.
    Bengtsson, Daniel
    et al.
    Linnaeus University.
    Safi, Kamran
    Tyskland.
    Avril, Alexis
    Linnaeus University.
    Fiedler, Wolfgang
    Tyskland.
    Wikelski, Martin
    Tyskland.
    Gunnarsson, Gunnar
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Research environment Man & Biosphere Health (MABH).
    Elmberg, Johan
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Research environment Man & Biosphere Health (MABH).
    Tolf, Conny
    Linnaeus University.
    Olsen, Björn
    Uppsala University.
    Waldenström, Jonas
    Linnaeus University.
    Does influenza A virus infection affect movement behaviour during stopover in its wild reservoir host?2016In: Royal Society Open Science, E-ISSN 2054-5703, Vol. 3, no 2, article id 150633Article in journal (Refereed)
    Abstract [en]

    The last decade has seen a surge in research on avian influenza A viruses (IAVs), in part fuelled by the emergence, spread and potential zoonotic importance of highly pathogenic virus subtypes. The mallard (Anas platyrhynchos) is the most numerous and widespread dabbling duck in the world, and one of the most important natural hosts for studying IAV transmission dynamics. In order to predict the likelihood of IAV transmission between individual ducks and to other hosts, as well as between geographical regions, it is important to understand how IAV infection affects the host. In this study, we analysed the movements of 40 mallards equipped with GPS transmitters and three-dimensional accelerometers, of which 20 were naturally infected with low pathogenic avian influenza virus (LPAIV), at a major stopover site in the Northwest European flyway. Movements differed substantially between day and night, as well as between mallards returning to the capture site and those feeding in natural habitats. However, movement patterns did not differ between LPAIV infected and uninfected birds. Hence, LPAIV infection probably does not affect mallard movements during stopover, with high possibility of virus spread along the migration route as a consequence.

  • 88. Bengtsson, Daniel
    et al.
    Waldenström, Jonas
    Elmberg, Johan
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Research environment Man & Biosphere Health (MABH).
    Gunnarsson, Gunnar
    Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap.
    Änders rörelser kartlagda2014In: Vår fågelvärld, ISSN 0042-2649, Vol. 73, no 5, p. 46-48Article in journal (Other (popular science, discussion, etc.))
  • 89.
    Bengtsson, Mikael
    Kristianstad University College, Department of Mathematics and Science.
    Vattenkvarnar - en studie om Hjul- och skvaltkvarnar och deras förekomst i Fulltofta socken 2005Independent thesis Basic level (degree of Bachelor)Student thesis
    Abstract [sv]

    This rapport handles the presence of two kinds of watermills in the Swedish landscape. The first is a small type with a horizontal shovel blade and the other a bigger kind with a vertical standing shovel scoop. The rapport seeks for information of their capacity and how long they existed as flour producing units. A important issue is how small can a brook be and still run a mill. Finally the reasons for their disappearance in one selected parish is shown.

  • 90.
    Bengtsson, Sandra
    Kristianstad University, School of Health and Society.
    “Utan sociala medier har jag varit supersocial”: En netnografisk studie av vad som sker när nätgenerationens aktiva användare av sociala medier går offline.2016Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    Much of people's everyday communication is today digital, taking place in a virtual world in which people are often more present than in the actual world. This paper deals with the consequences of active users of the net generation going offline from social media for a week. The results in this paper are based on empirical data gathered through a netnographic study carried out by observing and following 12 participants' experiences on how they were affected by being without social media for a week. Data generated in this study is the result of the participants' personal diaries, 12 completed surveys and two interviews. The purpose of the study is to find out what happens when you go against the grain in today's society and log out: How you will be affected and how it is received by the suroundings. The study shows that the participants found it difficult to be without social media at the beginning, but that they, after the study, could see several positive effects with being offline. The majority of the respondents experienced some relief in not being constantly available online. The results of this study show that the use of social media is mostly a routine habit, which you can manage without. The participants of this study have lacked communication through social media in several job and school contexts, otherwise they don’t believe that they have missed something of greater value online during the time as offline. The result also shows that the majority of the participants felt better to be offline than online, and that they will be more observant of their social media use in the future and try to cut it down. The participants consider it to be much more worthwhile to be present in the real world than in the virtual world, that they has been more social without social media. This study is an important contribution of knowledge for designers in digital technology, as studies addressing non-use is a relatively unexplored area. Mapping this kind of the non-use can be a good method to help designers to design good and user-friendly design in the future. I see this study as a preliminary study for future research of non-use where the study is ongoing for a long time.

  • 91.
    Bengtsson, Torbjörn
    et al.
    Örebro University.
    Khalaf, Atika
    Kristianstad University, School of Health and Society, Avdelningen för Hälsovetenskap II. Kristianstad University, Research Environment PRO-CARE.
    Khalaf, Hazem
    Örebro University.
    Secreted gingipains from P. gingivalis colonies exert potent immunomodulatory effects on human gingival fibroblasts2015In: Microbiological Research, ISSN 0944-5013, Vol. 178, p. 18-26Article in journal (Refereed)
    Abstract [en]

    Periodontal pathogens, including Porphyromonas gingivalis, can form biofilms in dental pockets and cause inflammation, which is one of the underlying mechanisms involved in the development of periodontal disease, ultimately leading to tooth loss. Although P. gingivalis is protected in the biofilm, it can still cause damage and modulate inflammatory responses from the host, through secretion of microvesicles containing proteinases. The aim of this study was to evaluate the role of cysteine proteinases in P. gingivalis colony growth and development, and subsequent immunomodulatory effects on human gingival fibroblast. By comparing the wild type W50 with its gingipain deficient strains we show that cysteine proteinases are required by P. gingivalis to form morphologically normal colonies. The lysine-specific proteinase (Kgp), but not arginine-specific proteinases (Rgps), was associated with immunomodulation. P. gingivalis with Kgp affected the viability of gingival fibroblasts and modulated host inflammatory responses, including induction of TGF-β1 and suppression of CXCL8 and IL-6 accumulation. These results suggest that secreted products from P. gingivalis, including proteinases, are able to cause damage and significantly modulate the levels of inflammatory mediators, independent of a physical host-bacterial interaction. This study provides new insight of the pathogenesis of P. gingivalis and suggests gingipains as targets for diagnosis and treatment of periodontitis.

  • 92.
    Bengtsson, Åsa
    et al.
    Kristianstad University College.
    Jönsson, Åsa
    Kristianstad University College.
    Naturreservatet Kullan: inventering av kärlväxter och naturvärdesbedömning2007In: Tsunami, ISSN 1651-5803, no 1Article in journal (Other (popular science, discussion, etc.))
    Abstract [sv]

    Olofströms kommun är på gång att starta ett ”Markernas museum” och i samband med detta fick vi i uppdrag av Olofströms kommun att inventera kärlväxterna i Kullans naturreservat. Detta tillsammans med en inventering av geologi som gjorts av Kamil Al-Ajely och de kulturella värdena som gjorts av Thomas Persson ska ligga som grund för ”Markernas museum”. Inventeringen till kommunen gjordes vid tre tillfällen, juni-augusti, 2005 och en rapport om detta lämnades in i september 2005 . Eftersom vi skulle ha material till vårt examensarbete har vi gjort ytterligare undersökningar än vad kommunen anvisade. Ytterligare en inventering har gjorts i maj 2006 för att få med vårväxterna. Naturreservatet Kullan är ett 41 ha stort område som blev naturreservat 2003. Den enda inventering som enligt uppgift gjorts är en artinventering från slutet av 1980-talet. Från denna ska det finnas en artlista, enligt inventeraren själv och länsstyrelsen, dock har det inte gått att få tag på den trots upprepade försök. Området som inventerats består av flera olika biotoper, ädellövskog, naturbetesmarker, kärr, slåtterängar och en sjö. Kullan har en lång kontinuitet som hävdad mark vilket ligger som grund för den stora artrikedomen. Det mesta av området har varit täckt av lövskog som förmodligen fungerat som betesmark ända fram till början av 1900-talet. Då ägdes gården av Åke och Bengta Svensson. Områdena runt sjön består av mosse eller kärr och i början av 1900-talet användes en del av våtmarkerna som åker. På kartan från 1915-17 ser man att sjön sänkts jämfört med föregående karta. Idag betas delar av området av 20-talet nötkreatur från vår till höst. Syfte och frågeställningarDen varierande omgivningen och det faktum att området hyser över 200 arter kärlväxter, gjorde att vi blev intresserade av att undersöka området. • Hur stora naturvärden finns här och hur skiljer sig vegetationen åt mellan de olika biotoperna?• Hur brukades Kullan under 1900-talet?• Hur såg markanvändningen ut under 1800-talet och hur har kontinuiteten av hävden sett ut?• Hur står sig Kullan jämfört med andra liknande områden?

  • 93.
    Berg, Johan
    et al.
    RISE.
    Wendin, Karin
    Kristianstad University, School of Education and Environment, Avdelningen för Mat- och måltidsvetenskap. Kristianstad University, Research Environment Food and Meals in Everyday Life (MEAL).
    Langton, Maud
    Sveriges Lantbruksuniversitet.
    Josell, Åsa
    Atria Scandinavia AB.
    Davidsson, Fredrik
    Geoloc AB.
    State Of The Art report -: insects as food and feed2017In: Annals of Experimental Biology, ISSN 2348-1935, Vol. 5, no 2, p. 1-9Article in journal (Refereed)
    Abstract [en]

    FAO has considered insects as food since 2003 [1] and is promoting consumption of insects (entomophagy) in the Western world because of the possibilities for sustainable production it offers. Insects as food are considered to leave smaller ecological footprints than conventional livestock (beef, pigs, and poultry) regarding feed, land and water needs, as well as greenhouse gas and ammonia emissions [2-7]. As an example regarding water, taken from a recent TV documentary, if a family of four people got their protein need from insects one day a week instead of from conventional livestock, over a year they would save the planet about a million Liters of water [8]. This is in agreement with figures above.

  • 94.
    Bergentz, Cornelia
    Kristianstad University, School of Health and Society.
    Gamification och miljö: vägen till miljövänligare transportval via motiverande spelmekanismer2016Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    Former human activities that happened in the past affect the climate change that we see today. An environmentally friendly choice in an urban environment is to use public transport, but cars and buses emit amounts of carbon dioxide and hazardous particles. The ideal would have been to get more people to choose a bike instead of a car or public transport because the use of bicycles does not affect the environment or human health negative. A study was conducted on the gamification mechanics in order to change people’s behavior. The target group belonged mostly to the user category “free spirits” but they were also motivated by game mechanics from every user category. The results from the study conclude that if one should make use of gamification in this area then they need to work in different ways to approach different audiences and to find different groups of gamers. One should also find various game mechanics that motivate the users to be able to develop the most optimal platform for motivation.

  • 95.
    Bergman, Sanne
    Kristianstad University, School of Education and Environment.
    Genetic analysis of the otter population (Lutra lutra) in Kristianstad’s Vattenrike Biosphere Reserve, Sweden2017Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    During the past century the Swedish otter (Lutra lutra) population showed a rapid decline in abundance and genetic diversity. Among the most affected areas was the southern province of Skåne. After prohibiting hunting of otters and banning harmful pollutants like PCB, Swedish populations slowly recovered. To some areas the otter returned late, like Kristianstad in north-eastern Skåne. Here, the River Helge å enters Kristianstad’s Vattenrike, Biosphere Reserve and forms a biodiverse wetland. By 2011, otters had established once more along the river. In recent years, a female otter with cubs have appeared outside Vattenriket visitor’s centre “naturum”, to the joy of inhabitants and visitors. In Kristianstad, otters have become a recurring winter attraction. However, not much is known about this new population. For assessment of abundance and genetic diversity, microsatellite variation was investigated among now-living individuals from eleven sites in the Biosphere reserve, and stored museum samples from ten otters with origins in North-eastern Skåne. Using a non-invasive methodology, investigated DNA was extracted from faeces and muscle tissue from dead individuals. Multiple replicate screening was performed to detect errors in genotyping procedures. Results show the presence of three now-living individuals (two males and one female). Now-living otters and museum specimens from north-eastern Skåne were not closely related. Sampled individuals show Hardy-Weinberg Equilibrium, but their heterozygosity is very low. Results suggest that, even though some individuals may remain undetected, low admixture of new genes may be a cause for concern. For long-term protection and management in Kristianstad’s Vattenrike, Biosphere Reserve, further knowledge is needed about our new and precious otter population.

  • 96.
    Bergström, Staffan
    et al.
    Kristianstad University College, Department of Mathematics and Science.
    Svensson, Britt-Marie
    Kristianstad University College, Department of Mathematics and Science.
    Mårtensson, Lennart
    Kristianstad University College, Department of Mathematics and Science.
    Mathiasson, Lennart
    Department of Analytical Chemistry, Lund University.
    Development and application of an analytical protocol for evaluation of treatment processes for landfill leachates: I. Development of an analytical protocol for handling organic compounds in complex leachate samples2007In: International Journal of Environmental Analytical Chemistry, ISSN 0306-7319, E-ISSN 1029-0397, Vol. 87, no 1, p. 1-15Article in journal (Refereed)
    Abstract [en]

    A strategy is presented for evaluation of treatment procedures for landfill leachate with emphasis on organic pollutants. An analytical scheme, the LAQUA protocol, was developed as a guide for the analytical work. The protocol includes organic as well as metals,. inorganic ions, water-quality parameters, and toxicity. The proposed strategy considers the behaviour of both polar and non-polar organic substances at trace levels. For polar substances, phenols were chosen as markers and determined with an automated supported liquid membrane extraction device, coupled on-line to HPLC with a diode-array detector. For non-polar substances, PCBs and 10 unidentified compounds were chosen as markers and analysed by solid-phase extraction combined with supercritical fluid extraction with GC analysis. The chosen measurement strategy, based on the use of marker substances, difference measurements, and versatile data-handling procedures, provided essential information about complex systems at relatively low cost.

  • 97.
    Berlin, Camilla
    Kristianstad University College, Department of Mathematics and Science.
    Rening av oljeförorenat vatten med torvabsorbent2007Independent thesis Basic level (degree of Bachelor)Student thesis
    Abstract [sv]

    Hässleholms Renhållare AB är ett avfallsbolag som innehar en oljebehandlingsanläggning på deras centrala avfallsanläggning i Vankiva. På oljebehandlingsanläggningen tar de emot slam från oljeavskiljare och bilvårdsanläggningar samt i mindre mängd förorenade massor. Från behandlingsanläggningen utgår det en vattenfas som leds via ett avloppssystem vidare till det lokala behandlingssystemet för lakvattnet.

    I bolagets tillstånd för Vankiva avfallsanläggning har det föreskrivits att varje delflöde till den lokala behandlingen av lakvattnet måste kontrolleras för att inte fastställda gränsvärden för olja och tungmetaller ska överskridas.

    Under våren 2006 kompletterades avloppssystemet från oljebehandlingsanläggningen till lakvattenbehandlingen med en filterbrunn. Filterbrunnen innehåller en torvabsorbent som suger upp olja och tungmetaller från vattenfasen. Genom att reducera olja och tungmetaller från vattenfasen kommer bolaget att minska en stor källa av föroreningar till deras lokala behandlingssystem för lakvatten.

    Rapportens mål var att undersöka filtermaterialets reningsförmåga på vattenfasen från oljebehandlingsanläggning dels vid laborativa försök och dels vid fältundersökning.

    De laborativa försöken bestod av ett urlakningstest och en studie på filtermaterialets absorptionskapacitet. Resultat från urlakningstestet visar att torvsorbenten släpper mangan och järn i större mängd och sänker urlakningsvätskans pH. Resultat från filtermaterialets absorptionskapacitet visar att materialet effektivt adsorberar undersökta metaller. Ett utläckage av arsenik sker från filtermaterialet. För olja och dess biprodukter sker en mindre reduktion.

    Fältförsöket visar att filtret reducerar metallkoncentrationen i utgående vattenfas men absorption av olja uteblir vid hög belastning. Lakvattnet får tillskott av metalljoner från vattenfasen av aluminium, koppar, järn, mangan, nickel, bly och zink, då metallkoncentrationen är högre för vattenfasen än för lakvattnet.

    Kontroll av basflödet, som har ett konstant mindre flöde igenom filterbrunnen, visar resultat på låga metallkoncentrationer i ingående basflöde, som består av vatten från området tvätthall och avloppsvatten från kontoret. När basflödet passerar filterbrunnen urlakas arsenik, kvicksilver, nickel och zink från adsorberade metalljoner i filtermaterialet.

    Vid fältstudien visar resultaten på att filterbrunnen fungerade som en buffert. Filtermaterialet sorberade föroreningar i ingående vattenfas från oljeplattan och släppte metaller när basflödet passerade filterbrunnen. Detta kan medföra att det inte blir några stötbelastningar till lakvattnet av befintliga föroreningar från vattenfasen men tungmetaller kommer för eller senare ut i behandlingssystemet för lakvattnet.

  • 98.
    Berntsson, Marcus
    Kristianstad University, School of Health and Society.
    Penetrationstest2014Independent thesis Basic level (university diploma), 5 credits / 7,5 HE creditsStudent thesis
    Abstract [sv]

    Det här arbete behandlar grunderna i penetrationstestning, vilket är en metod föratt kontrollera säkerheten i datorer och nätverk genom att försöka sätta in sig isamma tankesätt och metoder som en potentiell angripare kan ha.Den teoritiska delen går igenom processen för hur ett penetrationstest kan gå tilloch vad som är viktigt att veta och att tänka på under penetrationstestet.Det presenteras även några populära och användbara verktyg som kananvändas för penetrationstestning, nämligen Nmap, Backtack, Nessus ochMetasploit, samt en nogrann beskrivning av några av de mest vanligtvisförekommande allvarliga sårbarheterna.I genomförandedelen utförs ett penetrationstest i en labmiljö, och sätter då deninformation i den teoritiska delen i praktik. De verktygen som används gåsigenom mer detaljerat och olika metoder för att använda dessa verktyg ipenetrationstestningssyfte testas.Penetrationstestet gås igenom stegvis, och börjar med att hitta maskiner pånätverket och även hitta detaljerad information och möjligheter för angrepp. Deninformationen används sedan för att hitta sårbarheter i maskinerna, och slutligenutnyttjas dessa sårbarheter för att installera ett remote-access program och gekontrollen till den angripande datorn.

  • 99.
    Bertilsson, Mattias
    et al.
    Kristianstad University College, School of Engineering.
    Lindberg, Johan
    Kristianstad University College, School of Engineering.
    Affärssystem för Gamersneed Sweden2007Independent thesis Basic level (degree of Bachelor)Student thesis
    Abstract [sv]

    This degree project deals with the creation of a business system for Gamersneed Sweden.

    The system is divided into two parts, one windows appliction and one websolution.

  • 100.
    Bertolani, Roberto
    et al.
    Italien.
    Guidetti, Roberto
    Italien.
    Jönsson, K. Ingemar
    Lund University.
    Altiero, Tiziana
    Italien.
    Boschini, Deborah
    Rebecchi, Lorena
    Italien.
    Experiences with dormancy in tardigrades2004In: Journal of limnology, ISSN 1129-5767, E-ISSN 1723-8633, Vol. 63, no Suppl. 1, p. 16-25Article in journal (Refereed)
    Abstract [en]

    Tardigrades often colonise extreme habitats, in which they survive using both types of dormancy: quiescence and diapause. Together with nematodes and bdelloid rotifers, tardigrades are known to enter quiescence (with several forms of cryptobiosis: anhydrobiosis, cryobiosis, anoxybiosis, osmobiosis) at any stage of their life cycle, from egg to adult. Entering anhydrobiosis, tardigrades contract their body into a so-called tun, loosing most of their free and bound water (>95%), synthesizing cell protectants (e.g., trehalose, glycerol, heat shock proteins) and strongly reducing or suspending their metabolism. Our research on cryptobiosis focused on some ecological and evolutionary aspects. We evaluated: i) the long-term anhydrobiotic survival by comparing quantitative data on recovery from naturally induced desiccation in several species of tardigrades; ii) differences in survival patterns between species and populations by experimentally inducing anhydrobiosis and cryobiosis; iii) phenotypic factors affecting anhydrobiotic survival. As regards diapause, we considered encystment and eggs. Encystment involves at least the synthesis of new cuticular structures. Morphological changes during cyst formation are more complex than those involved in tun formation. We analyzed more in detail encystment processes, comparing a semiterrestrial with a limnic species. Several inter-specific differences have been identified, other than the production of two types of cysts in the semiterrestrial species. Our analysis of life history traits of a laboratory reared strain of a soil tardigrade revealed a particular hatching phenology that involved the production of both subitaneous and resting eggs. The latter need a cue to hatch (dehydration followed by re-hydration). In addition, the evolutionary meaning of dormancy in tardigrades is discussed

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