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  • 1.
    Heydorn, Per
    et al.
    Lunds universitet.
    Anderbrant, Olle
    Lunds universitet.
    Jonsson, Lars J.
    Kristianstad University, Faculty of Natural Science, Research environment Man & Biosphere Health (MABH). Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
    Svensson, Glenn P.
    Lunds universitet.
    Long-term rearing affects pheromone-mediated flight behaviour of the Indian meal moth, Plodia interpunctella2019In: Journal of applied entomology, ISSN 0931-2048, E-ISSN 1439-0418Article in journal (Refereed)
    Abstract [en]

    The pest Plodia interpunctella (Hubner) is reared in many research laboratories. In a culture established in 1996, attraction of males to the female-produced sex pheromone in flight tunnel assays gradually decreased after approximate to 15 years of rearing. A new culture was established to enable comparison with the old culture regarding traits associated with mate finding. Female calling activity, pheromone titre and male antennal response to pheromone components did not differ between cultures. In contrast, very few males from the old culture reached the pheromone source in flight tunnel assays compared with 61%-81% of males from the other culture. Our results highlight the importance of maintaining viable insect cultures for research purposes and suggest frequent evaluation of traits involved in chemical communication in such cultures to ensure reliable results in experiments.

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