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  • 1.
    Bohn, Pernille
    et al.
    Toxicology Laboratory, Analytical Biosciences, Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen.
    Bak, Søren A.
    Eurofins Denmark A/S, Environment, Vejen.
    Björklund, Erland
    Högskolan Kristianstad, Sektionen för lärande och miljö, Avdelningen för Naturvetenskap. Högskolan Kristianstad, Plattformen för molekylär analys. Högskolan Kristianstad, Fakulteten för naturvetenskap, Avdelningen för miljö- och biovetenskap. Högskolan Kristianstad, Fakulteten för naturvetenskap, Forskningsmiljön MoLab.
    Krogh, Kristine A.
    Toxicology Laboratory, Analytical Biosciences, Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen.
    Hansen, Martin
    Toxicology Laboratory, Analytical Biosciences, Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen.
    Abiotic degradation of antibiotic ionophores2013Inngår i: Environmental Pollution, ISSN 0269-7491, E-ISSN 1873-6424, Vol. 182, s. 177-183Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Hydrolytic and photolytic degradation were investigated for the ionophore antibiotics lasalocid, monensin, salinomycin, and narasin. The hydrolysis study was carried out by dissolving the ionophores in solutions of pH 4, 7, and 9, followed by incubation at three temperatures of 6, 22, and 28 °C for maximum 34 days. Using LC–MS/MS for chemical analysis, lasalocid was not found to hydrolyse in any of the tested environments. Monensin, salinomycin, and narasin were all stable in neutral or alkaline solution but hydrolysed in the solution with a pH of 4. Half-lives at 25 °C were calculated to be 13, 0.6, and 0.7 days for monensin, salinomycin, and narasin, respectively. Absorbance spectra from each compound indicated that only lasalocid is degraded by photolysis (half-life below 1 h) due to an absorbance maximum around 303 nm, and monensin, salinomycin, and narasin are resistant to direct photolysis because they absorb light of environmentally irrelevant wavelengths.

  • 2.
    Rodriguez-Navas, Carlos
    et al.
    Danmark.
    Björklund, Erland
    Högskolan Kristianstad, Sektionen för lärande och miljö, Avdelningen för Naturvetenskap. Högskolan Kristianstad, Plattformen för molekylär analys. Högskolan Kristianstad, Fakulteten för naturvetenskap, Avdelningen för miljö- och biovetenskap. Högskolan Kristianstad, Fakulteten för naturvetenskap, Forskningsmiljön MoLab.
    Halling-Sørensen, Bent
    Danmark.
    Hansen, Martin
    Danmark.
    Biogas final digestive byproduct applied to croplands as fertilizer contains high levels of steroid hormones2013Inngår i: Environmental Pollution, ISSN 0269-7491, E-ISSN 1873-6424, Vol. 180, s. 368-371Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    In this study we evaluate and demonstrate the occurrence of nine natural and one synthetic steroid hormone, including estrogens, androgens and progestagens in biogas final digestate byproduct (digestion liquid) commonly used as an agricultural fertilizer. We investigated two biogas sites that utilize different anaerobic digestion technologies (mesophilic and thermophilic) from swine manure and other organic wastes. Individual hormone concentration levels were observed up to 1478 ng g−1 dry weight or 22.5 mg kg−1 N with estrone and progesterone reaching highest concentration levels. Evaluation of the potential environmental burden through the application in agriculture was also assessed on the basis of predicted environmental concentrations. This study indicates that the biogas digestion process does not completely remove steroid hormones from livestock manure and use of final digestate byproduct on croplands contributes to the environmental emission of hormones.

  • 3. Tessier, L
    et al.
    Boisvert, J L
    Vought, Lena B. M.
    Lunds universitet.
    Lacoursière, Jean O.
    Lunds universitet.
    Anomalies on capture nets of Hydropsyche slossonae larvae (Trichoptera; Hydropsychidae) following a sublethal chronic exposure to cadmium2000Inngår i: Environmental Pollution, ISSN 0269-7491, E-ISSN 1873-6424, Vol. 108, nr 3, s. 425-438Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    A laboratory study on the sublethal effects of cadmium (Cd) on the net-spinning process of the larvae of Hydropsyche slossonae was conducted in order to assess the potential of net anomalies as an indicator of chronic exposure to Cd. Two major anomalies with different frequency levels were identified after chronic exposure to 0.37, 1.2, 11.6, 21.4 and 43.3 μg l−1 of Cd. The first was a distortion of the midline meshes where the diamond-shape structure is disrupted and the meshes are separated by extra strands (called ‘midline’ anomaly). The second aberration consisted of a distortion of the rectilinear structure of net opening by strands being fused or added over the meshes (called ‘crossover’ anomaly). The midline distortion may be linked to a physiological stress caused by Cd, which can affect the control of the net-spinning process. It was not possible to relate the crossover aberrations to a specific toxic action of Cd, but data indicated that both anomalies are independent from each other and that two modes of action could be implicated. Protein analyses of capture nets have revealed silk polypeptide modifications at the highest Cd concentration tested, indicating a possible effect of Cd interaction with silk proteins. However, neither a gradient-concentration nor a time-dependent response could be established with both aberration frequencies. Silk protein modifications would rather play a secondary role in the appearance of both net anomalies, and mostly at a high concentration level. Finally, the toxicity curves (EC50) show that the sensitivity threshold for both types of aberration ranged from 1 to 5 μg l−1 which is highly sensitive compared with other sublethal effects of Cd on other macroinvertebrate species. Hence, the use of capture-net anomalies of hydropsychid larvae would represent a valuable indicator of sublethal toxicity induced by Cd and possibly by other metals in running waters.

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