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  • 1.
    Lind-Halldén, Christina
    et al.
    Kristianstad University, Department of Mathematics and Science.
    Halldén, Christer
    Department of Clinical Chemistry, Malmö University Hospital.
    Säll, T.
    Department of Genetics, Lund University.
    Genetic variation in Arabidopsis suecica and its parental species A. arenosa and A. thaliana2002In: Hereditas, ISSN 0018-0661, E-ISSN 1601-5223, Vol. 136, no 1, p. 45-50Article in journal (Refereed)
    Abstract [en]

    Random amplified polymorphic DNA (RAPD) markers were used to estimate the level of genetic variation in Swedish accessions of the allopolyploid Arabidopsis suecica and its parental species A. thaliana and A. arenosa. The results showed clear differences among the three species with respect to the level of variation. A. arenosa was highly variable, A. thaliana showed a moderate level of variation whereas A. suecica was much less variable than the two other species. An extended analysis covering 19 Swedish populations of A. suecica corroborated the low level of variation in this species, yet 16 unique phenotypes were observed. No isolation by distance was observed. When the genetic variation was partitioned among and within populations of A. suecica, the results showed that the majority of the variation (81%) occurred among populations. This result is interpreted as a strong indication that A. suecica is autogamous in nature.

  • 2.
    Säll, T.
    et al.
    Department of Genetics, Lund University.
    Lind-Halldén, Christina
    Kristianstad University, Department of Mathematics and Science.
    Halldén, Christer
    Department of Clinical Chemistry, Malmö University Hospital,.
    Primer mixtures in RAPD analysis2000In: Hereditas, ISSN 0018-0661, E-ISSN 1601-5223, Vol. 132, no 3, p. 203-208Article in journal (Refereed)
    Abstract [en]

    RAPD (random amplified polymorphic DNA) is a multiplex marker system that conventionally uses single-primer PCR to amplify random DNA fragments. Because of its multiplex nature, it is frequently used in bulked segregant analysis (BSA). In view of the very large numbers of markers BSA often requires, we investigated the use of mixtures of primers as a method of increasing the number of markers available. Theoretically, if a single-primer reaction produces x bands on average, an unrestrained PCR process using a primers should produce xa2 bands. Initially, we investigated mixtures containing from one to five primers. The average number of products increased slightly from the single-primer to the multiple-primer case, whereas it was rather constant for the different multi-primer combinations. This deviation from the theoretical expectations, which we attribute to the effects of competition, shows mixtures of more than two primers to be inefficient. The properties of two-primer mixtures in which the proportions of the two primers were varied were also investigated. The intensities of most of the products were influenced by the proportions of the primers used to create the mixture. A good fit was obtained to a model in which the average competitive ability of a band is directly proportional to the probability of randomly obtaining the band-producing primer combination from the pool of primers. Using two-primer mixtures, a(a-1)/2 different two-primer mixtures can be produced. A comparison of different schemes for constructing the two-primer mixtures indicates that the degree of resampling is similar for all schemes. In conclusion, the use of two-primer mixtures is a simple but very powerful strategy in BSA as it can generate an extremely large number of markers.

  • 3.
    Säll, Torbjörn
    et al.
    Department of Cell and Organism Biology, Genetics, Lund University.
    Lind-Halldén, Christina
    Kristianstad University, Department of Mathematics and Science.
    Jakobsson, Mattias
    Department of Cell and Organism Biology, Genetics, Lund University.
    Halldén, Christer
    Department of Clinical Chemistry, Malmö University Hospital.
    Mode of reproduction in Arabidopsis suecica2004In: Hereditas, ISSN 0018-0661, E-ISSN 1601-5223, Vol. 141, no 3, p. 313-317Article in journal (Refereed)
    Abstract [en]

    The breeding system of Arabidopsis suecica was investigated through genetic analysis of microsatellite segregation patterns in five controlled crosses as well as in 16 single-mother families collected in the wild. Analysis of single and two-locus segregations in the F2 generation following a cross clearly shows that A. suecica is reproduces sexually. The single-mother families show a high level of homozygosity corroborating earlier results indicating a high level of inbreeding. The high level of individual homozygosity is due both to a high level of selfing and to the underlying population structure.

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