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  • 1. Grey, Carl
    et al.
    Widen, Cecilia
    Department of Plant Breeding and Biotechnology Balsgård, Swedish University of Agricultural Sciences, Kristianstad.
    Adlercreutz, Patrick
    Rumpunen, Kimmo
    Duan, Rui-Dong
    Antiproliferative effects of sea buckthorn (Hippophae rhamnoides L.) extracts on human colon and liver cancer cell lines2010In: Food Chemistry, ISSN 0308-8146, E-ISSN 1873-7072, Vol. 120, no 4, p. 1004-1010Article in journal (Refereed)
    Abstract [en]

    Sea buckthorn berries contain many bioactive compounds that have anticancer properties. To investigate whether the anti proliferative effects Could be associated with the presence of certain compounds. a sequential extraction was performed. The extraction started with heptane followed by ethyl acetate, ethanol, and water. A second protocol using ethanol:water (1:1) was also used. The contents of the extracts were determined and their effects on cell proliferation were investigated in both Caco-2 and Hep G2 cells. The ethyl acetate fraction was exclusively found to contain high levels of ursolic acid, together with low amounts of phenolics. The ethanol:water extracts contained high levels of phenolic compounds and proanthyocyanidin, but little ursolic acid. When the antiproliferative effects were examined, the strongest inhibitory effect was found in the ethyl acetate extract for the Caco-2 cells and in the ethanol:water extract for the Hep G2 cells. The antiproliferative effects were in both cases dose-dependent and were in the case of the ethyl acetate extract associated with an increase in apoptosis. The results obtained show that the choice of extraction solvent is of considerable importance and that ursolic acid might be more important than the polyphenols in inhibiting the cancer cell proliferation. (C) 2009 Elsevier Ltd. All rights reserved.

  • 2.
    Wendin, Karin
    et al.
    SIK, The Swedish Institute for Food and Biotechnology AB.
    Langton, M
    Caous, L
    Hall, C
    Dynamic analyses of sensory and microstructural properties of cream cheese2000In: Food Chemistry, ISSN 0308-8146, E-ISSN 1873-7072, Vol. 71, no 3, p. 363-378Article in journal (Refereed)
    Abstract [en]

    Flavour and texture in cream cheese depend on the microstructure. The objective of this work was to study the influence of fat content, salt content and homogenisation pressure on the microstructure and sensory properties of cream cheese. Twelve types of cream cheese were produced according to a full-factorial design, whereby the fat content was set at three levels, the salt content at two levels and the homogenisation pressure at two levels. The cheeses were analysed by a sensory panel, using both quantitative descriptive profiling and time intensity (T1) evaluation, and by using a confocal laser scanning microscope, CLSM, whereby the microstructure of the cheeses was analysed. All the design parameters had a significant influence on the flavour and texture, although fat had the largest effect. Interaction effects between the design parameters were also found to influence the character of cream cheese. The results showed that it is possible to create a cream cheese with lower fat content and with sensory attributes similar to the attributes in cream cheese with high fat content, by modification of production parameters.

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