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A novel method for analysing key corticosteroids in polar bear (Ursus maritimus) hair using liquid chromatography tandem mass spectrometry
Danmark.
Danmark.ORCID iD: 0000-0002-4663-8742
Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap.ORCID iD: 0000-0003-2283-2927
Danmark.
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2016 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 1017, 45-51 p.Article in journal (Refereed) Published
Abstract [en]

This paper presents the development and evaluation of a methodology for extraction, clean-up and analysis of three key corticosteroids (aldosterone, cortisol and corticosterone) in polar bear hair. Such a methodology can be used to monitor stress biomarkers in polar bears and may provide as a useful tool for long-term and retrospective information. We developed a combined pressurized liquid extraction (PLE)-solid phase extraction (SPE) procedure for corticosteroid extraction and clean-up followed by high pressure liquid chromatography tandem mass spectrometry (HPLC-MS/MS) analysis. This procedure allows for the simultaneous determination of multiple steroids, which is in contrast to previous polar bear studies based on ELISA techniques. Absolute method recoveries were 81%, 75% and 60% for cortisol, corticosterone and aldosterone, respectively. We applied the developed method on a hair sample pooled from four East Greenland polar bears. Herein cortisol and corticosterone were successfully determined in levels of 0.32 +/- 0.02 ng/g hair and 0.13 +/- 0.02 ng/g hair, respectively. Aldosterone was below limit of detection (LOD<0.17 ng/g). The cortisol hair concentration found in these East Greenland polar bears was consistent with cortisol levels previously determined in the Southern Hudson Bay and James Bay in Canada using ELISA kits. (C) 2016 Elsevier B.V. All rights reserved.

Place, publisher, year, edition, pages
2016. Vol. 1017, 45-51 p.
Keyword [en]
PLE, SPE LC-MS/MS, polar bear hair, fur, cortisol, corticosterone, aldosterone
National Category
Medical Biotechnology
Identifiers
URN: urn:nbn:se:hkr:diva-15965DOI: 10.1016/j.jchromb.2016.02.029ISI: 000373547200006PubMedID: 26945133OAI: oai:DiVA.org:hkr-15965DiVA: diva2:968436
Available from: 2016-09-12 Created: 2016-09-12 Last updated: 2016-09-12Bibliographically approved

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CiteExportLink to record
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Citation style
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