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Induction of morphological changes in death-induced cancer cells monitored by holographic microscopy
Malmö University.
Phase Holographic Imaging AB, Lund.
Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Research environment Man & Biosphere Health (MABH).ORCID iD: 0000-0003-4190-5431
Finland.
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2015 (English)In: Journal of Structural Biology, ISSN 1047-8477, E-ISSN 1095-8657, Vol. 189, no 3, 207-212 p.Article in journal (Refereed) Published
Abstract [en]

We are using the label-free technique of holographic microscopy to analyze cellular parameters including cell number, confluence, cellular volume and area directly in the cell culture environment. We show that death-induced cells can be distinguished from untreated counterparts by the use of holographic microscopy, and we demonstrate its capability for cell death assessment. Morphological analysis of two representative cell lines (L929 and DU145) was performed in the culture flasks without any prior cell detachment. The two cell lines were treated with the anti-tumour agent etoposide for 1-3days. Measurements by holographic microscopy showed significant differences in average cell number, confluence, volume and area when comparing etoposide-treated with untreated cells. The cell volume of the treated cell lines was initially increased at early time-points. By time, cells decreased in volume, especially when treated with high doses of etoposide. In conclusion, we have shown that holographic microscopy allows label-free and completely non-invasive morphological measurements of cell growth, viability and death. Future applications could include real-time monitoring of these holographic microscopy parameters in cells in response to clinically relevant compounds.

Place, publisher, year, edition, pages
2015. Vol. 189, no 3, 207-212 p.
Keyword [en]
Digital holographic microscopy, Volume, Cell death, Viability, Cell morphology, Imaging
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:hkr:diva-13491DOI: 10.1016/j.jsb.2015.01.010ISI: 000351249500005PubMedID: 25637284OAI: oai:DiVA.org:hkr-13491DiVA: diva2:786795
Available from: 2015-02-06 Created: 2015-02-06 Last updated: 2016-08-10Bibliographically approved

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Tassidis, Helena
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Avdelningen för NaturvetenskapResearch environment Man & Biosphere Health (MABH)
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CiteExportLink to record
Permanent link

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Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
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  • nn-NO
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  • Other locale
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Output format
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