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Asymmetrical flow field-flow fractionation enables the characterization of molecular and supramolecular properties of cereal beta-glucan dispersions
Department of Food Technology, Engineering and Nutrition, Faculty of Engineering LTH, Lund University. (Food and Meal Science)ORCID iD: 0000-0002-0002-661X
Biomedical Nutrition, Pure and Applied Biochemistry, Faculty of Engineering LTH, Lund University.
Department of Food Technology, Engineering and Nutrition, Faculty of Engineering LTH, Lund University.
2012 (English)In: Carbohydrate Polymers, ISSN 0144-8617, E-ISSN 1879-1344, Vol. 87, no 1, p. 518-523Article in journal (Refereed) Published
Abstract [en]

In this paper we study the properties of molecular and supra molecular species in cereal beta-glucan solutions/dispersions by the utilization of asymmetrical flow field-flow fractionation coupled to multi-angle light scattering and refractive index (AsFIFFF-MALS-RI) detectors. The samples were purified barley and oat beta-glucans which were dissolved in aqueous solution using either mild conditions or more harsh treatments with alkali. Dissolution in 0.5 M NaOH was not sufficient to eliminate aggregated structures in barley beta-glucan. The results in this paper show how distinction can possibly be made between molecular and supra molecular species using scaling approaches and conformational parameters obtained from AsFIFFF-MALS-RI over the entire size distribution. Small species in the barley beta-glucan samples display properties ranging from elongated conformation to random coil conformation. Aggregates have low apparent densities and a swollen micro gel structure. Oat beta-glucan displays no properties that can be attributed to a molecularly dissolved beta-glucan showing that dissolution was incomplete. The aggregate properties analyzed were similar between oat and barley beta-glucan.

Place, publisher, year, edition, pages
2012. Vol. 87, no 1, p. 518-523
Keywords [en]
Beta-glucan, Asymmetrical flow field-flow fractionation, AF4, Oat, Barley
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:hkr:diva-12384DOI: 10.1016/j.carbpol.2011.08.014ISI: 000297389000068OAI: oai:DiVA.org:hkr-12384DiVA, id: diva2:733585
Available from: 2014-07-10 Created: 2014-07-10 Last updated: 2017-12-05Bibliographically approved

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Håkansson, Andreas

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CiteExportLink to record
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  • apa
  • harvard1
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