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Similarities in the subgingival microbiota assessed by a curet sampling method at sites with chronic periodontitis.
University of Bern, Bern, Switzerland & University of Washington, Seattle, WA & University of Washington.ORCID iD: 0000-0002-3620-5978
University of Bern, Bern, Switzerland.
University of Bern, Bern, Switzerland.
University of Bern.
2008 (English)In: Journal of Periodontology, ISSN 0022-3492, Vol. 79, no 12, 2290-2296 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: The goal of this study was to determine whether site-specific differences in the subgingival microbiota could be detected by the checkerboard method in subjects with periodontitis.

METHODS: Subjects with at least six periodontal pockets with a probing depth (PD) between 5 and 7 mm were enrolled in the study. Subgingival plaque samples were collected with sterile curets by a single-stroke procedure at six selected periodontal sites from 161 subjects (966 subgingival sites). Subgingival bacterial samples were assayed with the checkerboard DNA-DNA hybridization method identifying 37 species.

RESULTS: Probing depths of 5, 6, and 7 mm were found at 50% (n = 483), 34% (n = 328), and 16% (n = 155) of sites, respectively. Statistical analysis failed to demonstrate differences in the sum of bacterial counts by tooth type (P = 0.18) or specific location of the sample (P = 0.78). With the exceptions of Campylobacter gracilis (P <0.001) and Actinomyces naeslundii (P <0.001), analysis by general linear model multivariate regression failed to identify subject or sample location factors as explanatory to microbiologic results. A trend of difference in bacterial load by tooth type was found for Prevotella nigrescens (P <0.01). At a cutoff level of > or = 1.0 x 10(5), Porphyromonas gingivalis and Tannerella forsythia (previously T. forsythensis) were present at 48.0% to 56.3% and 46.0% to 51.2% of sampled sites, respectively.

CONCLUSIONS: Given the similarities in the clinical evidence of periodontitis, the presence and levels of 37 species commonly studied in periodontitis are similar, with no differences between molar, premolar, and incisor/cuspid subgingival sites. This may facilitate microbiologic sampling strategies in subjects during periodontal therapy.

Place, publisher, year, edition, pages
2008. Vol. 79, no 12, 2290-2296 p.
Keyword [en]
recruitment, recruitment attempt, science, scientists, reproduction, identity
National Category
Dentistry
Identifiers
URN: urn:nbn:se:hkr:diva-12225DOI: 10.1902/jop.2008.080142ISI: 000261710900011PubMedID: 19053919OAI: oai:DiVA.org:hkr-12225DiVA: diva2:728509
Available from: 2014-06-24 Created: 2014-06-24 Last updated: 2014-09-18Bibliographically approved

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Citation style
  • apa
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