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Quantification of periodontal pathogens in vascular, blood and subgingival samples from patients with peripheral arterial disease or abdominal aortic aneurysms
University Complutense, Madrid.
Kristianstad University, School of Health and Society, Avdelningen för Hälsovetenskap III. Kristianstad University, Forskningsmiljön Oral Hälsa - Allmänhälsa - Livskvalitet.
University Complutense, Madrid.
Kristianstad University, School of Health and Society, Avdelningen för Hälsovetenskap III. Kristianstad University, Forskningsmiljön Oral Hälsa - Allmänhälsa - Livskvalitet.ORCID iD: 0000-0003-0992-2362
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2014 (English)In: Journal of Periodontology, ISSN 0022-3492, E-ISSN 1943-3670, Vol. 85, no 9, 1182-1193 p.Article in journal (Refereed) Published
Abstract [en]

Background: The aim of this investigation was to quantify periodontal pathogens (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Campylobacter rectus and Tannerella forsythia) in vascular, blood and subgingival samples. As secondary objective, two molecular bacterial identification methods [nested-polimerase chain reaction (PCR) and quantitative PCR (qPCR)] were compared.

Methods: Seventy consecutive patients provided a vascular lesion, a blood sample, and 36 subgingival samples. Bacterial deoxyribonucleic acid (DNA) was extracted and qPCR was used to determine the prevalence and amounts of the target pathogens in each sample. Nested-PCR was only performed in the samples from vascular lesions. Periodontal examination was performed in 42 patients. U-Mann-Whitney or Chi-squared tests were used to compare microbiological results according to periodontal diagnosis.

Results: All targeted periodontal pathogens (A. actinomycetemcomitans, P. gingivalis, T. forsythia or C. rectus) were detected in subgingival samples with a prevalence rate of 72.2%, 47.2%, 74.3% and 82.9%, respectively. In 7.1% and 11.4% of vascular and blood samples, bacterial DNA was detected. One patient was positive for A. actinomycetemcomitans in the three types of samples. No differences were found in the levels of targeted bacteria when comparing periodontitis and non-periodontitis patients. Prevalence rates obtained with nested PCR were significantly higher than those obtained by qPCR.

Conclusions: The presence of of A. actinomycetemcomitans was demonstrated in vascular, blood and subgingival samples in one out of 36 patients. These results, although with a very low frequency, may support the hypothesis of a translocation of periodontal pathogens from subgingival microbiota to the blood stream and then to atheromatous plaques in carotid or other peripheral arteries. Nested-PCR is not an adequate method for identifying DNA of periodontal pathogens in low quantities, due to the high number of false negative results.

Place, publisher, year, edition, pages
2014. Vol. 85, no 9, 1182-1193 p.
Keyword [en]
Real-time polymerase chain reaction, periodontal diseases, cardiovascular diseases, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia
National Category
Dentistry
Identifiers
URN: urn:nbn:se:hkr:diva-11781DOI: 10.1902/jop.2014.130604ISI: 000341580700010PubMedID: 24502612OAI: oai:DiVA.org:hkr-11781DiVA: diva2:696948
Available from: 2014-02-16 Created: 2014-02-16 Last updated: 2017-12-06Bibliographically approved

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Lindahl, ChristelRenvert, StefanOhlsson, Ola

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Avdelningen för Hälsovetenskap IIIForskningsmiljön Oral Hälsa - Allmänhälsa - LivskvalitetAvdelningen för Hälsovetenskap I
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Journal of Periodontology
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