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Identification of Vibrio anguillarum in fish by using partial 16S rRNA sequences and a specific 16S rRNA oligonucleotide probe
Department of Microbiology, Universitv of Umeå. (Man & Biosphere Health (MABH))ORCID iD: 0000-0002-8059-0156
Department of Microbiology, Universitv of Umeå.
Department of Microbiology, Universitv of Umeå.
Department of Microbiology, Universitv of Umeå.
1989 (English)In: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 55, no 8, 1907-1910 p.Article in journal (Refereed) Published
Abstract [en]

16S rRNA from seven different Vibrio anguillarum strains was partially sequenced and compared. From this sequence information we could design a 25-base-long oligonucleotide and use it as a specific probe for identification of V. anguillarum. This was determined by RNA-DNA colony hybridization and slot-blot hybridization. Strong, specific hybridization to the probe was observed for all V. anguillarum strains tested. Furthermore, no cross-hybridization could be seen against five other bacterial species. The detection limit was 5 x 10(3) bacteria per ml. It was even possible to detect V. anguillarum, by slot-blot hybridization, directly in a homogenized kidney from a fish that had died of vibriosis. The partial sequence information revealed small but significant differences between strains of the same species. These sequence differences are sufficiently significant to allow serotyping on the RNA level. Comparing strains of different serotypes revealed a 10-base and an 11-base difference in V. anguillarum serotypes O8 and O9, respectively, in a 122-base partial sequence.

Place, publisher, year, edition, pages
1989. Vol. 55, no 8, 1907-1910 p.
National Category
Microbiology
Identifiers
URN: urn:nbn:se:hkr:diva-10325ISI: A1989AH86600009PubMedID: 2782871OAI: oai:DiVA.org:hkr-10325DiVA: diva2:611853
Available from: 2013-03-19 Created: 2013-03-19 Last updated: 2014-06-24Bibliographically approved
In thesis
1. Use of 16S ribosomal RNA probes for the detection of marine bacteria
Open this publication in new window or tab >>Use of 16S ribosomal RNA probes for the detection of marine bacteria
1994 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Umåe: Univ., 1994. 35 p.
National Category
Microbiology
Identifiers
urn:nbn:se:hkr:diva-10356 (URN)91-7174-888-1 (ISBN)
Available from: 2013-04-03 Created: 2013-04-03 Last updated: 2014-06-24Bibliographically approved

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