The main aim of this research was to investigate four humour styles, affiliative, self-enhancing, aggressive, and self-defeating humour, and compare these with emotional perception. Forty-eight persons took part in the research, 27 women and 21 men living in the southern part of Sweden. Two humour tests were used: the Humour Scale Questionnaire – HSQ, with 32 items, and a newly composed video test, assembled after pilot testing from 26 video clips, the Humour Video Questionnaire – HVQ. To measure perception and interpretation of emotion, the revised version of the ‘Reading the Mind in the Eyes’ Test was used. In the HVQ, women appreciated self-defeating humour more than men did. Appreciation of aggressive humour in HVQ unexpectedly correlated with perception of emotions but positive humour style, in HSQ, also correlated with perception of emotions. Overall women tended to appreciate humour more than men did.