These days, with the emerging developments in wireless communication technologies, such as 6G and 5G and the Internet of Things (IoT) sensors, the usage of E-Transport applications has been increasing progressively. These applications are E-Bus, E-Taxi, self-autonomous car, E-Train and E-Ambulance, and latency-sensitive workloads executed in the distributed cloud network. Nonetheless, many delays present in cloudlet-based cloud networks, such as communication delay, round-trip delay and migration during the workload in the cloudlet-based cloud network. However, the distributed execution of workloads at different computing nodes during the assignment is a challenging task. This paper proposes a novel Multi-layer Latency (e.g., communication delay, round-trip delay and migration delay) Aware Workload Assignment Strategy (MLAWAS) to allocate the workload of E-Transport applications into optimal computing nodes. MLAWAS consists of different components, such as the Q-Learning aware assignment and the Iterative method, which distribute workload in a dynamic environment where runtime changes of overloading and overheating remain controlled. The migration of workload and VM migration are also part of MLAWAS. The goal is to minimize the average response time of applications. Simulation results demonstrate that MLAWAS earns the minimum average response time as compared with the two other existing strategies.