hkr.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Bacterial invasion of dentinal tubules beneath apparently intact but hypomineralized enamel in molar teeth with molar incisor hypomineralization
Pediatric Dentistry Special Dental Service, Sahlgrenska University Hospital, Mölndal.
Kristianstad University College, Department of Health Sciences.
Department of Oral Pathology, Institute of Odontology, Sahlgrenska Academy, Göteborg University.
Centre of Electron Microscopy, Friedrich-Schiller-University, Jena.
Show others and affiliations
2008 (English)In: International Journal of Paediatric Dentistry, ISSN 0960-7439, Vol. 18, no 5, 333-340 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: The most common problems for a patient with molar incisor hypomineralization (MIH) are the collapse of enamel and cavitations, loss of fillings, and secondary caries, but most of all, severe hypersensitivity. OBJECTIVE: The aim of this paper was therefore to histologically study possible bacterial invasion of dentinal tubules beneath apparently intact, but hypomineralized enamel in permanent molars with MIH. MATERIAL AND METHODS: Five extracted permanent first molars diagnosed with MIH were fixated, demineralized, and sagittally serially sectioned in a bucco-lingual direction in a microtome with a thickness of 4-5 microm. Sections were stained with a modified Brown and Benn staining for bacteria, unstained sections were analysed in field emission SEM. RESULTS: Stained sections from the cuspal areas, below the hypomineralized enamel, the staining indicated the presence of bacteria in the dentinal tubules. The HTX staining showed that the pulp in sections without any findings was normal and free from bacteria or infiltrates from inflammatory cells. In sections where bacteria were found in the cuspal areas or deeper in the dentin, a zone of reparative dentin was found, and in sections from one tooth, the coronal pulp showed an inflammatory reaction with inflammatory cells. In sections adjacent to those without any bacterial staining, the SEM analyses revealed empty dentinal tubules without any odontoblast processes or signs of bacteria. When odontoblast processes were found, the dentinal tubules were filled with bacteria located on the surface of the odontoblast processes. In some areas, a large number of tubules were found with bacteria. No bacteria were found close to the pulp. The odontoblast processes appeared larger in areas where bacteria were found. CONCLUSIONS: The presence of bacteria in the dentinal tubules and inflammatory reactions in the pulp indicate that oral bacteria may penetrate through the hypomineralized enamel into the dentin, thus possibly contribute to hypersensitivity of teeth with MIH.

Place, publisher, year, edition, pages
2008. Vol. 18, no 5, 333-340 p.
Keyword [en]
TRANSMISSION ELECTRON-MICROSCOPY, PERMANENT 1ST MOLARS, ODONTOBLAST PROCESS, PREVALENCE, CHILDREN
National Category
Dentistry Dentistry
Identifiers
URN: urn:nbn:se:hkr:diva-27DOI: 10.1111/j.1365-263X.2007.00908.xISI: 000258326700003PubMedID: 18328044OAI: oai:DiVA.org:hkr-27DiVA: diva2:132595
Available from: 2008-12-22 Created: 2008-12-22 Last updated: 2009-03-18Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed
By organisation
Department of Health Sciences
In the same journal
International Journal of Paediatric Dentistry
DentistryDentistry

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 50 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf