Tobacco use is high in India. Previous studies have shown that awareness of health risks of tobacco use, and adolescents’ behavior are strongly associated with adolescents’ knowledge and perceptions towards tobacco use. There are only a few studies being conducted that assess school going boys’ and girls’ tobacco-related health knowledge, and their awareness related to tobacco control programmes. A questioner base cross-sectional study with 276 students had been conducted from four different schools (2 private and 2 government) in Ahmedabad district, Gujarat state, India. The results include from the group comparison (hypothesis testing) between the boys and girls. More than two thirds of boys’ and girls’ participants agreed tobacco use (also in all forms) is harmful to the body including the transitory use of the tobacco and its products. More than one third boys and one quarter girls believed that media plays an important role in promoting anti-tobacco messages. Around 50 % of the girls preferred strict anti-tobacco laws and policies in curbing the tobacco pandemic.  More than two thirds of the boys and girls in the study sample had no knowledge of any organizations or programs that assisted individuals to get rid of the tobacco habit. This study gives an overview of the knowledge regarding the tobacco use among the young school going boys and girls. Promoting school and community-based intervention programs and reviewing of the existing anti-tobacco policies could prove to be beneficial in curbing the tobacco consumption habit in adolescents.