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A novel method for calibration of AF4 channels for hydrodynamic radius determination: the nanoemulsion method (featuring MALS)
Lund University.
Lund University.
Lund University.
Lund University.
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2018 (English)In: Journal of Chromatography A, ISSN 0021-9673, Vol. 1533, p. 155-163Article in journal (Refereed) Published
Abstract [en]

This study suggests a novel method for determination of the channel height in asymmetrical flow field-flow fractionation (AF4), which can be used for calibration of the channel for hydrodynamic radiusdeterminations. The novel method uses an oil-in-water nanoemulsion together with multi angle lightscattering (MALS) and elution theory to determine channel height from an AF4 experiment. The methodis validated using two orthogonal methods; first, by using standard particle elution experiments and, sec-ondly, by imaging an assembled and carrier liquid filled channel by x-ray computed tomography (XCT). It is concluded that the channel height can be determined with approximately the same accuracy aswith the traditional channel height determination technique. However, the nanoemulsion method canbe used under more challenging conditions than standard particles, as the nanoemulsion remains stablein a wider pH range than the previously used standard particles. Moreover, the novel method is also morecost effective.

Place, publisher, year, edition, pages
2018. Vol. 1533, p. 155-163
Keyword [en]
Flow field-flow fractionation; AF4; Multi angle light scattering; Nanoemulsion; X-ray computed tomography; Channel height
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:hkr:diva-17707DOI: 10.1016/j.chroma.2017.12.026PubMedID: 29248348OAI: oai:DiVA.org:hkr-17707DiVA: diva2:1167223
Available from: 2017-12-18 Created: 2017-12-18 Last updated: 2018-01-10Bibliographically approved

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Håkansson, Andreas
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Research Environment Food and Meals in Everyday Life (MEAL)Avdelningen för Mat- och måltidsvetenskap
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CiteExportLink to record
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Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
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  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
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