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ESBL resistance patterns among environmental and clinical bacterial isolates
Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Research environment Man & Biosphere Health (MABH).ORCID iD: 0000-0002-8059-0156
Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Research environment Man & Biosphere Health (MABH).ORCID iD: 0000-0003-0724-2718
2017 (English)Conference paper, Oral presentation with published abstract (Other academic)
Abstract [en]

Objectives

We have studied the phenotypic and genetic pattern among Extended Spectrum Beta-lactamase (ESBL) producing bacteria in the aquatic environment, and have compared the result with clinical samples from the same area.

Methods

Water samples have been collected at three different sites in the Helge river, Kristianstad community, Sweden. The first station is located before the outlet from the municipal sewage plant, the second just after the outlet and the third close to the Baltic Sea. Cultured bacterial isolates from the water and clinical isolates were analysed for phenotypic expression of ESBL related genes using the MAST-test, and genetically by PCR analyses of a set of ESBL genes, i.e. blaCTX-M, blaSHV, blaTEM, and blaOXA  

Results

Both clinical and environmental ESBL isolates were dominated by Escherichia coli and Klebsiella pneumoniae. Highest abundance of environmental ESBL isolates were obtained from the station close to the sewage outlet, the lowest close to the Baltic Sea. The gene cluster blaCTX-M was the most common among all isolates (65%), followed by blaTEM (30%).  The blaOXA and blaSHV genes were more common in clinical isolates.

Conclusion

A majority of the ESBL bacteria were mediated by chromosomal genes, dominated by blaCTX-M. However, blaOXA .and blaSHV were more common in clinical isolates. Further genetic analyses will be performed on more isolates, and on total bacterial community DNA.

 

Place, publisher, year, edition, pages
2017.
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:hkr:diva-17536OAI: oai:DiVA.org:hkr-17536DiVA: diva2:1150032
Conference
Focused Meeting 2017: Antimicrobial Resistance and One Health 29 - 30 August 2017
Available from: 2017-10-17 Created: 2017-10-17 Last updated: 2017-10-17Bibliographically approved

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Rehnstam-Holm, Ann-SofiAxelsson, Carolina
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CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
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  • asciidoc
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