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Visualisering av amyloider och patogenes i skadad näthinna
Kristianstad University, School of Education and Environment.
2017 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [sv]

Ansamling av amyloid beta (Aβ) i de extracellulära miljöerna är associerad till många svåra sjukdomar som Alzheimers och ålders-relaterad makuladegeneration (AMD). Amyloider karaktäriseras av att de är olösliga, toxiska mot neuron och orsakar därför svår skada. AMD är den ledande orsaken till blindhet och irreversibelt förlorande av skarp syn då Aβ manifesterar i makula. I AMD orsakar Aβ inflammatorisk aktivitet där det retinala pigmentepitelet bryts ned och ljuskänsliga fotoreceptorer dör genom apoptos. Idag lever ca 150 miljoner människor med AMD där mänga har svårt att utföra vardagliga uppgifter till följd av förlust av skarp syn.

Idag är Kongo röd en av de vanligaste metoderna för att visualisera amyloider in vitro. Den patogenes som orsakas av amyloider kan analyseras med immunofluorescens och immunohistokemi.

Syftet med studien var att undersöka förekomst av amyloider i samband med celldöd i näthinna från gris, undersöka den patogenes som amyloider orsakar med immunofluorescens och immunohistokemi, samt undersöka om det finns korrelation mellan amyloider och celldöd. Resultatet visade att amyloider var förekommande i näthinnan och hade orsakat celldöd och ansamling av aggresomer. Amyloider och den patologi som orsakats kunde visualiseras i det yttre lagret av näthinnan.

Abstract [en]

Deposition of amyloid beta (Aβ) in the extracellular environment are associated to some severe diseases, like Alzheimer’s disease and age-related macular degeneration (AMD).

Amyloids are characterized by insolubility, toxicity towards neuron and are there-for damaging to tissues. AMD is the primary cause of blindness and irreversible loss of central vision through manifestation of Aβ in the macula. In AMD, Aβ drives an inflammatory action that degenerates the retinal pigment epithelium and cause atrophy of photoreceptors.

Today ~150 million people live with AMD where many find difficulties performing everyday tasks due to loss of sharp vision.

Congo red is a gold standard for visualizing amyloids in vitro and the pathogenesis caused by amyloids can be analyzed by immunofluorescence and immunohistochemistry.

The purpose of this study was to show the presence of amyloids relating to cell death in pig retina, show the pathogenesis caused by amyloids by using immunofluorescence and immunohistochemistry, and investigate whether there is correlation between amyloids and cell death.

The result showed that amyloids were present in the retina and caused cell death and gathering of aggresomes.

Amyloids and the caused pathology could be visualized in the outer layer of the retina.

Place, publisher, year, edition, pages
2017. , p. 13
Keywords [en]
Amyloid, Congo red, TUNEL, ubiquitin, ProteoState, Age-related macular degeneration
Keywords [sv]
Amyloid, Kongo röd, TUNEL, ubiquitin, ProteoStat, Ålders-relaterad makular degeneration
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:hkr:diva-17218OAI: oai:DiVA.org:hkr-17218DiVA, id: diva2:1139160
Educational program
Bachelor of Science in Biomedical Laboratory Science
Supervisors
Examiners
Available from: 2017-09-21 Created: 2017-09-06 Last updated: 2017-09-21Bibliographically approved

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CiteExportLink to record
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