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Jämförelse av två enzyme-linked immunosorbent assays: mätning av diabetesspecifika autoantikroppar i en adult population
Kristianstad University, Faculty of Natural Science, Avdelningen för miljö- och biovetenskap.
2018 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesisAlternative title
Comparison of two enzyme-linked immunosorbent assays : measurement of diabetes-specific autoantibodies in an adult population (English)
Abstract [sv]

Typ- 1 diabetes (T1D) är en autoimmun sjukdom med insulinbrist orsakad av nedbrytning av insulinproducerande betaceller i pankreas. Fyra olika antikroppar har identifierats som är riktade mot betacellsspecifika antigen; insulinautoantikroppar (IAA), glutamic acid decarboxylase antibodies (GADA), islet antigen2-antikroppar (IA-2A) och antikroppar riktade mot zinktransportören 8 (ZnT8A). I denna studie gjordes en jämförelse av metoderna 2screen islet cell autoantibody ELISA-kit (RSR, Cardiff, UK) och 3screen islet cell autoantibody ELISA- kit (RSR, Cardiff, UK), vars brunnar är coatade med GAD65/IA-2 antigen respektive GAD65/IA-2/ZnT8 antigen, för att undersöka ifall dessa båda kit ger jämförbar sensitivitet och specificitet i en adult population av nydebuterade patienter med T1D och friska vuxna blodgivare. RSR 2screen erhöll 1 % högre specificitet (98 %) jämfört med RSR 3screen (97 %) vid samma sensitivitet (92 %) och rekommenderas i första hand för screening av autoantikroppar i en population av vuxna patienter med ökad risk för T1D och friska vuxna blodgivare.

Abstract [en]

Type- 1 diabetes (T1D) is an autoimmune disease with insulin deficiency caused by degradation of insulin- producing betacells in pancreas. Four different autoantibodies that target beta- cell specific antigenes have been identified: insulinautoantibodies (IAA), glutamic acid decarboxylase antibodies (GADA), islet antigen2-antibodies (IA-2A) and antibodies against zinktransporter 8 (ZnT8A). In this study, a comparison between 2screen islet cell autoantibody ELISA-kit (RSR, Cardiff, UK) coated with GAD65/IA-2 and 3screen islet cell autoantibody ELISA- kit (RSR, Cardiff, UK) coated with GAD65/IA-2/ZnT8, was performed to investigate whether results from these two kits provide comparable sensitivity and specificity in an adult population of new onset patients with T1D and healthy adults. RSR 2screen obtained 1 % higher specificity (98 %) in comparison to RSR 3screen (97 %) on the same sensitivity (92 %) and is recommended primarily for screening of autoantibodies in a population of adult patients at increased risk for T1D and healthy adults blood donors.

Place, publisher, year, edition, pages
2018. , p. 36
Keywords [en]
ELISA, typ 1 diabetes, autoantibodies, ROC-curve, sensitivity, specificity
Keywords [sv]
ELISA, typ 1 diabetes, autoantikroppar, ROC-kurva, sensitivitet, specificitet
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:hkr:diva-18698OAI: oai:DiVA.org:hkr-18698DiVA, id: diva2:1248504
External cooperation
Wallenbergs laboratoriet
Educational program
Bachelor of Science in Biomedical Laboratory Science
Presentation
Högskolan Kristianstad, Kristianstad (Swedish)
Supervisors
Examiners
Available from: 2018-09-24 Created: 2018-09-15 Last updated: 2018-09-24Bibliographically approved

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CiteExportLink to record
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Citation style
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