hkr.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Sequence analysis of the DNA-repair gene rad51 in the tardigrades Milnesium cf. tardigradum, Hypsibius dujardini and Macrobiotus cf. harmsworthi
Pontificia Universidad Javeriana, Instituto de Genética Humana, Bogotá.
Högskolan Kristianstad, Sektionen för lärande och miljö, Avdelningen för Naturvetenskap. Högskolan Kristianstad, Forskningsmiljön Man and Biosphere Health (MABH).ORCID-id: 0000-0002-1732-0372
Department of Genetics, Microbiology and Toxicology, Stockholm University.
Department of Genetics, Microbiology and Toxicology, Stockholm University.
Visa övriga samt affilieringar
2013 (Engelska)Ingår i: Journal of limnology, ISSN 1129-5767, E-ISSN 1723-8633, Vol. 72, nr s1, s. 80-91Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Tardigrades are known for being resistant to extreme conditions, including tolerance to ionising and UV radiation in both the hydratedand the dehydrated state. It is known that these factors may cause damage to DNA. It has recently been shown that single and double DNAstrand breaks occur when tardigrades are maintained for a long time in the anhydrobiotic state. This may suggest that perhaps tardigrades rely on efficient DNA repair mechanisms. Among all proteins that comprise the DNA repair system, recombinases such as RecA or Rad51 have a very important function: DNA exchange activity. This enzyme is used in the homologous recombination and allows repair of thedamaged strand using homologous non-damaged strands as a template. In this study, Rad51 induction was evaluated by western blot in Milnesium cf. tardigradum, after exposure to gamma radiation. The Rad51 protein was highly induced by radiation, when compared to the control. The rad51 genes were searched in three tardigrades: Milnesium cf. tardigradum, Hypsibius dujardini and Macrobiotus cf. harmsworthi. The gene sequences were obtained by preparing and sequencing transcriptome libraries for H. dujardini and M. cf. harmsworthi and designing rad51 degenerate primers specific for M. cf. tardigradum. Comparison of Rad51 putative proteins from tardigrades with other organisms showed that they are highly similar to the corresponding sequence from the nematode Trichinella spiralis. A structure-based sequence alignment from tardigrades and other organisms revealed that putative Rad51 predicted proteins from tardigrades contain the expected motifs for these important recombinases. In a cladogram tree based on this alignment, tardigrades tend to cluster together suggesting that they have selective differences in these genes that make them diverge between species. Predicted Rad51 structures from tardigrades were also compared with crystalline structure of Rad51 in Saccharomyces cerevisiae. These results reveal that S. cerevisiae Rad51 structure is very similar to that of the three analysed tardigrades. On the other hand the predicted structure of Rad51 from M. cf. harmsworthi and H. dujardini are closer related to each other, than each of them to that of M. cf. tardigradum.

Ort, förlag, år, upplaga, sidor
2013. Vol. 72, nr s1, s. 80-91
Nyckelord [en]
Rad51, phylogeny, Tardigrada, recombinase, DNA repair
Nyckelord [sv]
tardigrader, björndjur, strålningstolerans, DNA-skador, Rad51
Nationell ämneskategori
Biokemi och molekylärbiologi
Identifikatorer
URN: urn:nbn:se:hkr:diva-10794DOI: 10.4081/jlimnol.2013.s1.e10ISI: 000327485200010OAI: oai:DiVA.org:hkr-10794DiVA, id: diva2:638096
Tillgänglig från: 2013-07-26 Skapad: 2013-07-26 Senast uppdaterad: 2017-12-06Bibliografiskt granskad

Open Access i DiVA

fulltext(1775 kB)429 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 1775 kBChecksumma SHA-512
d1304dd229b1153905c47d3e2094412997e339384c8cc82e084d42472762ef76bce1986cc5d4e8265fe743b5053d2a1a53d62ce68af438910002f0ec79d5c66b
Typ fulltextMimetyp application/pdf

Övriga länkar

Förlagets fulltext

Personposter BETA

Jönsson, Ingemar

Sök vidare i DiVA

Av författaren/redaktören
Jönsson, Ingemar
Av organisationen
Avdelningen för NaturvetenskapForskningsmiljön Man and Biosphere Health (MABH)
I samma tidskrift
Journal of limnology
Biokemi och molekylärbiologi

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 429 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

doi
urn-nbn

Altmetricpoäng

doi
urn-nbn
Totalt: 823 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf