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Identification of Vibrio anguillarum in fish by using partial 16S rRNA sequences and a specific 16S rRNA oligonucleotide probe
Department of Microbiology, Universitv of Umeå. (Man & Biosphere Health (MABH))ORCID-id: 0000-0002-8059-0156
Department of Microbiology, Universitv of Umeå.
Department of Microbiology, Universitv of Umeå.
Department of Microbiology, Universitv of Umeå.
1989 (Engelska)Ingår i: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 55, nr 8, s. 1907-1910Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

16S rRNA from seven different Vibrio anguillarum strains was partially sequenced and compared. From this sequence information we could design a 25-base-long oligonucleotide and use it as a specific probe for identification of V. anguillarum. This was determined by RNA-DNA colony hybridization and slot-blot hybridization. Strong, specific hybridization to the probe was observed for all V. anguillarum strains tested. Furthermore, no cross-hybridization could be seen against five other bacterial species. The detection limit was 5 x 10(3) bacteria per ml. It was even possible to detect V. anguillarum, by slot-blot hybridization, directly in a homogenized kidney from a fish that had died of vibriosis. The partial sequence information revealed small but significant differences between strains of the same species. These sequence differences are sufficiently significant to allow serotyping on the RNA level. Comparing strains of different serotypes revealed a 10-base and an 11-base difference in V. anguillarum serotypes O8 and O9, respectively, in a 122-base partial sequence.

Ort, förlag, år, upplaga, sidor
1989. Vol. 55, nr 8, s. 1907-1910
Nationell ämneskategori
Mikrobiologi
Identifikatorer
URN: urn:nbn:se:hkr:diva-10325ISI: A1989AH86600009PubMedID: 2782871OAI: oai:DiVA.org:hkr-10325DiVA, id: diva2:611853
Tillgänglig från: 2013-03-19 Skapad: 2013-03-19 Senast uppdaterad: 2017-12-06Bibliografiskt granskad
Ingår i avhandling
1. Use of 16S ribosomal RNA probes for the detection of marine bacteria
Öppna denna publikation i ny flik eller fönster >>Use of 16S ribosomal RNA probes for the detection of marine bacteria
1994 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Ort, förlag, år, upplaga, sidor
Umåe: Univ., 1994. s. 35
Nationell ämneskategori
Mikrobiologi
Identifikatorer
urn:nbn:se:hkr:diva-10356 (URN)91-7174-888-1 (ISBN)
Tillgänglig från: 2013-04-03 Skapad: 2013-04-03 Senast uppdaterad: 2014-06-24Bibliografiskt granskad

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