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Collin, Betty
Publications (10 of 14) Show all publications
Collin, B. & Hernroth, B. (2020). Experimental evaluation of survival of Vibrio parahaemolyticus in fertilized cold-water sediment. Journal of Applied Microbiology
Open this publication in new window or tab >>Experimental evaluation of survival of Vibrio parahaemolyticus in fertilized cold-water sediment
2020 (English)In: Journal of Applied Microbiology, ISSN 1364-5072, E-ISSN 1365-2672Article in journal (Refereed) Epub ahead of print
Abstract [en]

AIMS: This experimental study focuses on survival and consistence of Vibrio parahaemolyticus in cold water sediments and how increasing temperature and nutritional availability can affect growth.

METHODS AND RESULTS: A pathogenic strain of V. parahaemolyticus was inoculated in seawater microcosms containing bottom sediment. Gradually, during 14 days, the temperature was upregulated from 8 to 21°C. Culturable V. parahaemolyticus were only found in the sediment but declined over time and did not recover even after another two days at 37°C. Numbers of culturable bacteria matched the amount found by q-PCR indicating that they did not enter a dormant state, contrary to those in the water layer. After adding decaying phytoplankton as fertilizer to the microcosms of 8 and 21°C for 7 and 14 days, the culturability of the bacteria increased significantly in the sediments at both temperatures and durations of exposure.

CONCLUSION: The study showed that V. parahaemolyticus can stay viable in cold water sediment and growth was stimulated by fertilizers rather than by temperature.

SIGNIFICANCE AND IMPACT OF THE STUDY: V. parahaemolyticus is a common cause of seafood-borne gastroenteritis and is today recognized in connection to increasing ocean temperature. The results indicate that this pathogen should be considered a risk in well-fertilized environments, such as aquacultures, even during cold periods.

Keywords
Vibrio parahaemolyticus, tdh+, climate change, microcosm experiment, seafood safety, sediment
National Category
Microbiology
Identifiers
urn:nbn:se:hkr:diva-20425 (URN)10.1111/jam.14618 (DOI)32086873 (PubMedID)
Available from: 2020-02-27 Created: 2020-02-27 Last updated: 2020-02-27Bibliographically approved
Collin, B. & Hernroth, B. (2020). Experimental evaluation of survival of Vibrio parahaemolyticus in fertilized cold‐water sediment. Journal of Applied Microbiology
Open this publication in new window or tab >>Experimental evaluation of survival of Vibrio parahaemolyticus in fertilized cold‐water sediment
2020 (English)In: Journal of Applied Microbiology, ISSN 1364-5072, E-ISSN 1365-2672, ISSN 1364-5072Article in journal (Refereed) Published
Abstract [en]

Aims: This experimental study focuses on survival and consistence of Vibrio parahaemolyticus in cold‐water sediments and how increasing temperature and nutritional availability can affect growth. Methods and Results: A pathogenic strain of V. parahaemolyticus was inoculated in seawater microcosms containing bottom sediment. Gradually, during 14 days, the temperature was upregulated from 8 to 21°C. Culturable V. parahaemolyticus was only found in the sediment but declined over time and did not recover even after another 2 days at 37°C. Numbers of culturable bacteria matched the amount found by q‐PCR indicating that they did not enter a dormant state, contrary to those in the water layer. After adding decaying phytoplankton as fertilizer to the microcosms of 8 and 21°C for 7 and 14 days, the culturability of the bacteria increased significantly in the sediments at both temperatures and durations of exposure. Conclusion: The study showed that V. parahaemolyticus can stay viable in cold‐water sediment and growth was stimulated by fertilizers rather than by temperature. Significance and Impact of the Study: Vibrio parahaemolyticus is a common cause of seafood‐borne gastroenteritis and is today recognized in connection to increasing ocean temperature. The results indicate that this pathogen should be considered a risk in well‐fertilized environments, such as aquacultures, even during cold periods.

Keywords
climate change, microcosm experiment, seafood safety, sediment, tdh+, Vibrio parahaemolyticus
National Category
Biological Sciences
Identifiers
urn:nbn:se:hkr:diva-20494 (URN)10.1111/jam.14618 (DOI)
Available from: 2020-03-23 Created: 2020-03-23 Last updated: 2020-03-23Bibliographically approved
Hernroth, B. & Collin, B. (2018). Kan klimatförändringen öka smitta av infektionssjukdomar via havsvatten?. In: Ann-Sofi Rehnstam Holm (Ed.), Man and Biosphere Health: en komplett akademisk miljö (pp. 22-33). Kristianstad: Högskolan Kristianstad
Open this publication in new window or tab >>Kan klimatförändringen öka smitta av infektionssjukdomar via havsvatten?
2018 (Swedish)In: Man and Biosphere Health: en komplett akademisk miljö / [ed] Ann-Sofi Rehnstam Holm, Kristianstad: Högskolan Kristianstad , 2018, p. 22-33Chapter in book (Other academic)
Abstract [sv]

De pågående klimatförändringarna gör haven allt varmare ochsurare. Dessutom förväntas perioder av intensiva regn att öka, v et bidrar till högre koncentrationer av näringsämnen i havsvattnet. Även sjukdomsframkallande bakterier och virus följer med vattnet från land ut i havet. Näringsämnena kommer att göda algblomningarna, vilket resulterar i fler syrefria bottnar. I bottensedimenten finns det naturligt rikligt med metallen mangan. Den frisätts till vattnet vid syrebrist och kan då tas upp av bottenlevande djur. I spåren av detta har vi genom experimentella långtidsstudier sett negativa effekter på immunförsvaret hos både kräfta, mussla och sjöstjärna. Förmågan att eliminera inkräktande bakterier och virus försämrades och djuren blev mer infektionsbenägna. Den tydligaste effekten såg vi då vi tillsatte mangan. Vi undersöker även hur viabilitet och virulens hos sjukdomsalstrande bakterier i havet påverkas och har bl.a. sett att havslevande bakterier av släktet Vibrio inte far illa av de förändrade faktorerna i havet, vilket indikerar att de kan få ett övertag på demarina organismerna, vars immunförsvar försvagats.

Place, publisher, year, edition, pages
Kristianstad: Högskolan Kristianstad, 2018
Series
Kristianstad University Press ; 11:2018
National Category
Biological Sciences Other Social Sciences
Identifiers
urn:nbn:se:hkr:diva-19177 (URN)978-91-87973-35-2 (ISBN)
Available from: 2019-03-18 Created: 2019-03-18 Last updated: 2019-03-18Bibliographically approved
Thorell, K., Collin, B., Hernroth, B. & Sjöling, Å. (2016). Complete genome sequences of two marine Vibrio cholerae strains isolated from the south coast of Sweden. Genome Announcements, 4(5), Article ID e01118-16.
Open this publication in new window or tab >>Complete genome sequences of two marine Vibrio cholerae strains isolated from the south coast of Sweden
2016 (English)In: Genome Announcements, ISSN 2169-8287, E-ISSN 2169-8287, Vol. 4, no 5, article id e01118-16Article in journal (Refereed) Published
Abstract [en]

Vibrio cholerae serogroups O1 and O139 are commonly associated with diarrhea, while non-O1-O139 strains may cause wound infections. Here, we present the genome sequences of two V. cholerae strains isolated from blue mussels (Mytilus edulis) collected in coastal waters of southern Sweden.

National Category
Microbiology in the medical area
Identifiers
urn:nbn:se:hkr:diva-16264 (URN)10.1128/genomeA.01118-16 (DOI)27789632 (PubMedID)
Funder
Swedish Research Council, 348-2014-2639
Available from: 2016-11-25 Created: 2016-11-25 Last updated: 2018-01-13Bibliographically approved
Collin, B., Rehnstam-Holm, A.-S., Ehn Börjesson, S.-M., Mussagy, A. & Hernroth, B. (2013). Characteristics of potentially pathogenic vibrios from subtropical Mozambique compared with isolates from tropical India and boreal Sweden. FEMS Microbiology Ecology, 83(2), 255-264
Open this publication in new window or tab >>Characteristics of potentially pathogenic vibrios from subtropical Mozambique compared with isolates from tropical India and boreal Sweden
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2013 (English)In: FEMS Microbiology Ecology, ISSN 0168-6496, E-ISSN 1574-6941, Vol. 83, no 2, p. 255-264Article in journal (Refereed) Published
Abstract [en]

Reported outbreaks of Vibrio parahaemolyticus have increased worldwide, particularly in regions of high seafood consumption. In Mozambique, seafood constitutes an important food resource and diarrheal diseases are common among its inhabitants. Edible clams were collected in Maputo Bay during both the dry and rainy seasons, with the results showing the number of viable counts of vibrios in clams to peak during the latter. Vibrio parahaemolyticus was the predominant species identified among the isolated strains. Although only one of 109 total strains carried the tdh virulence gene, 69% of isolates showed evidence of hemolytic capacity when subjected to a functional test. Similar virulence patterns and biochemical properties were found in strains isolated from Indian and Swedish marine waters. Antibiotic resistance was, however, more pronounced in strains isolated from these latter two environments.

Keywords
Vibrio spp, Vibrio parahaemolyticus, qPCR, probe hybridization, TCBS-agar, persistence in clams, PhenePlate system, antibiotic resistance, hemolysis
National Category
Microbiology
Identifiers
urn:nbn:se:hkr:diva-9610 (URN)10.1111/j.1574-6941.2012.01471.x (DOI)000313252600001 ()
Available from: 2012-08-22 Created: 2012-08-22 Last updated: 2020-02-28Bibliographically approved
Collin, B. (2012). Characterization and persistence of potential human pathogenic vibrios in aquatic environments. (Doctoral dissertation). Kristianstad: Kristianstad University
Open this publication in new window or tab >>Characterization and persistence of potential human pathogenic vibrios in aquatic environments
2012 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Vibrio spp., natural inhabitants of aquatic environments, are one of the most common causes of bacterial gastroenteritis in the world, being spread to humans via the ingestion of seafood, contaminated drinking water or exposure to seawater. The majority of Vibrio spp. are avirulent, but certain strains may sporadically be human pathogenic. Vibrio cholerae may cause cholera and fatal wound infections, Vibrio parahaemolyticus may cause gastroenteritis and Vibrio vulnificus may cause wound infections and sepsis. To expand current knowledge of the occurrence, ecological niche and persistence of potential human pathogenic Vibrio spp. in aquatic environments, occurrence and laboratory studies were performed. The seasonal variation of Vibrio spp. in clams and mussels from Mozambique and Sweden were studied, with isolated strains characterized and compared with those isolated from water samples collected in India. Results showed that the numbers of Vibrio spp. in Mozambican clams peaked during the warmer rainy season and that the dominating species was V. parahaemolyticus. Biochemical fingerprinting and virulence screened by PCR revealed a high similarity among strains from the different aquatic environments. However, isolate functional hemolytic analyses and antibiotic resistance patterns differed between strains; Swedish and Indian strains were less sensitive to the tested antibiotics and had a lower hemolytic capacity than those from Mozambique. Molecular analysis of bacterial DNA from Swedish mussels showed the presence of the three Vibrio spp. most commonly linked with human illness, as well as their associated virulence genes. The strains isolated from marine and clinical environments were equally and highly harmful to the tested eukaryotic cells. The persistence of clinical V. cholerae in aquatic environments was investigated in vivo. Strains were exposed to mussels, with bacterial uptake and elimination then examined. The mussels were able to avoid the most potent strain by complete closure of shells. The less potent strain was accumulated in mussel tissue in low levels and one marine control strain to a higher degree. Mussels eliminated the pathogenic strain less efficiently than they did the marine strain. One clinical and one marine strain were then exposed to 4°C for 21 days, with the temperature then increased to 20°C. The clinical strain was more prone to lose culturability than the marine strain at 4°C, the former performed significantly better in regaining culturability after the temperature up-shift. Subsequently, the persistence of the clinical strain in natural bottom sediment, incubating as above, was studied and results showed a similar decrease in culturable numbers in the sediment as in the water. As the clinical V. cholerae strains did not carry any of the standard set of virulence genes, the ability to change from non-culturable to culturable may be of great importance to strain pathogenicity. The results also show that natural bottom sediment may be a potential reservoir of human pathogenic Vibrio spp.

Place, publisher, year, edition, pages
Kristianstad: Kristianstad University, 2012
Keywords
Vibrio cholerae, Vibrio parahaemolyticus, Mozambique, Sweden, molluscs, occurrence, persistence, sediment, TCBS, PCR, PhP, antibiotic resistance
National Category
Microbiology
Identifiers
urn:nbn:se:hkr:diva-9789 (URN)978-­‐91-­‐628-­‐8482-­‐6 (ISBN)
Available from: 2012-10-17 Created: 2012-10-17Bibliographically approved
Collin, B., Rehnstam-Holm, A.-S., Lindmark, B., Pal, A., Wai, S. N. & Hernroth, B. (2012). The origin of Vibrio cholerae influences uptake and persistence in the blue mussel Mytilus edulis. Journal of Shellfish Research, 31(1), 87-92
Open this publication in new window or tab >>The origin of Vibrio cholerae influences uptake and persistence in the blue mussel Mytilus edulis
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2012 (English)In: Journal of Shellfish Research, ISSN 0730-8000, E-ISSN 1943-6319, Vol. 31, no 1, p. 87-92Article in journal (Refereed) Published
Abstract [en]

Vibrio cholerae may cause diarrheal diseases and wound infections, both of which have the potential to be fatal. Transmission to humans is often linked to consumption of contaminated shellfish/drinking water or dermal exposure to water (e.g. when swimming). In this study, we investigated whether different isolates of Vibrio cholerae differ in terms of accumulation, persistence, and viability when encountering blue mussels (Mytilus edulis). Mussel uptake and elimination of three different V. cholerae strains were compared: one fatal clinical non-O1/O139 isolate, one highly potent El Tor biotype, and one marine strain isolated from blue mussels. The results showed that the uptake of the marine strain was significantly higher than the clinical strain, but the elimination process of the marine strain was also more efficient. The El Tor strain was not at all ingested by the mussels. In addition, the survival of bacteria when incubated together with M. edulis hemocytes was tested in vitro. The viability of clinical strains was unaffected by the presence of hemocytes, and the marine strains were even more resistant and able to multiply. We conclude that the highly virulent El Tor biotype was not taken up by the mussels and could thereby escape the mussels' elimination process. The potentially fatal non-O1/O139 V. cholerae strain may accumulate in low numbers, but could be very persistent in mussels.

Keywords
mussel, Mytilus edulis, Vibrio cholerae, pathogenic, depuration, hemocytes
National Category
Biological Sciences
Identifiers
urn:nbn:se:hkr:diva-9251 (URN)10.2983/035.031.0111 (DOI)000302846800011 ()
Available from: 2012-05-02 Created: 2012-05-02 Last updated: 2017-12-07Bibliographically approved
Collin, B. & Rehnstam-Holm, A.-S. (2011). Occurrence and potential pathogenesis of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus on the South Coast of Sweden. FEMS Microbiology Ecology, 78(2), 306-313
Open this publication in new window or tab >>Occurrence and potential pathogenesis of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus on the South Coast of Sweden
2011 (English)In: FEMS Microbiology Ecology, ISSN 0168-6496, E-ISSN 1574-6941, Vol. 78, no 2, p. 306-313Article in journal (Refereed) Published
Abstract [en]

During the summer of 2006, several wound infections - of which three were fatal caused by Vibrio cholerae were reported from patients who had been exposed to water from the Baltic Sea. Before these reports, we initiated a sampling project investigating the occurrence of potential human pathogenic V. cholerae, Vibrio vulnificus and Vibrio parahaemolyticus in The Sound between Sweden and Denmark. The Blue mussel (Mytilus edulis) was used as an indicator to follow the occurrence of vibrios over time. Molecular analyses showed high frequencies of the most potent human pathogenic Vibrio spp.; 53% of mussel samples were positive for V. cholerae (although none were positive for the cholera toxin gene), 63% for V. vulnificus and 79% for V. parahaemolyticus (of which 47% were tdh(+) and/or trh(+)). Viable vibrios were also isolated from the mussel meat and screened for virulence by PCR. The mortality of eukaryotic cells when exposed to bacteria was tested in vivo, with results showing that the Vibrio strains, independent of species and origin, were harmful to the cells. Despite severe infections and several deaths, no report on potential human pathogenic vibrios in this area had been published before this study.

Keywords
Vibrio cholerae, Vibrio vulnificus, Vibrio parahaemolyticus, ecology, microbiology, wound infections, thermostable direct hemolysin, iii secretion system, wound-infection, mytilus-edulis, multiplex pcr, non-o1, temperature, bacteria, salinity, epidemiology
National Category
Microbiology
Identifiers
urn:nbn:se:hkr:diva-8790 (URN)10.1111/j.1574-6941.2011.01157.x (DOI)000296849000012 ()21692819 (PubMedID)
Available from: 2011-12-07 Created: 2011-12-06 Last updated: 2017-12-08Bibliographically approved
Rehnstam-Holm, A.-S. & Collin, B. (2011). Vibriobakterier i skånska vatten. Smittskydd Skåne (2), 3
Open this publication in new window or tab >>Vibriobakterier i skånska vatten
2011 (Swedish)In: Smittskydd Skåne, no 2, p. 3-Article in journal (Other academic) Published
National Category
Biological Sciences
Identifiers
urn:nbn:se:hkr:diva-8139 (URN)
Available from: 2011-06-16 Created: 2011-06-16 Last updated: 2014-06-24Bibliographically approved
Asplund, M. E., Rehnstam-Holm, A.-S., Atnur, V., Raghunath, P., Saravanan, V., Härnstrom, K., . . . Godhe, A. (2011). Water column dynamics of Vibrio in relation to phytoplankton community composition and environmental conditions in a tropical coastal area. Environmental Microbiology, 13(10), 2738-2751
Open this publication in new window or tab >>Water column dynamics of Vibrio in relation to phytoplankton community composition and environmental conditions in a tropical coastal area
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2011 (English)In: Environmental Microbiology, ISSN 1462-2912, E-ISSN 1462-2920, Vol. 13, no 10, p. 2738-2751Article in journal (Refereed) Published
Abstract [en]

Vibrio abundance generally displays seasonal patterns. In temperate coastal areas, temperature and salinity influence Vibrio growth, whereas in tropical areas this pattern is not obvious. The present study assessed the dynamics of Vibrio in the Arabian Sea, 1-2 km off Mangalore on the south-west coast of India, during temporally separated periods. The two sampling periods were signified by oligotrophic conditions, and stable temperatures and salinity. Vibrio abundance was estimated by culture-independent techniques in relation to phytoplankton community composition and environmental variables. The results showed that the Vibrio density during December 2007 was 10- to 100-fold higher compared with the February-March 2008 period. High Vibrio abundance in December coincided with a diatom-dominated phytoplankton assemblage. A partial least squares (PLS) regression model indicated that diatom biomass was the primary predictor variable. Low nutrient levels suggested high water column turnover rate, which bacteria compensated for by using organic molecules leaking from phytoplankton. The abundance of potential Vibrio predators was low during both sampling periods; therefore it is suggested that resource supply from primary producers is more important than top-down control by predators.

Keywords
real-time pcr, marine bacterioplankton, southwest coast, parahaemolyticus bacteria, culturable bacteria, chesapeake bay, arabian, sea, india, vulnificus, cholerae
National Category
Biological Sciences
Identifiers
urn:nbn:se:hkr:diva-8801 (URN)10.1111/j.1462-2920.2011.02545.x (DOI)000295971300011 ()21895909 (PubMedID)
Available from: 2011-12-06 Created: 2011-12-06 Last updated: 2017-12-08Bibliographically approved
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